Cyclosporine A Effect the CD28 Expression on CD8+T Cells of Patients with Aplastic Anemia through down Regulate FLIP.
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Aplastic anemia
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Sir: A number of investigators have recently reported that cell membranes, or solubilized proteins derived from them, are capable of inducing cytotoxic responses in secondary cultures (1–6). They have implied thereby that they are gaining insight into the specificity of the structures recognized by cytotoxic T cells. An alternative interpretation seems equally plausible: that the membrane preparations act not directly on cytotoxic memory cells, but rather on helper T cells, which, by secreting soluble mediators, “trigger” cytotoxic cell differentiation. This interpretation is supported by several recent reports. Thus, Ryser et al. (7), Wagner and Rollinghoff (8), and this laboratory (Okada et al. , in press) have shown that cell mediators secreted by primed Lyt I+ T cells can cause the direct differentiation of cytotoxic T cells in secondary (memory) cultures. Moreover, the specificity of the cytotoxic cells induced by these mediators is that of the antigen initially used for priming.
Priming (agriculture)
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To investigate the relationship between expression of Fas-FasL and apoptosis of peripheral blood CD8+CD28-and CD8+CD28+T lymphocytes in patients with systemic lupus erythematosus(SLE),the rates of Fas-FasL expression and apoptosis of CD8+CD28-and CD8+CD28+ T cells were measured by three antibodies labeling flow cytometry(FCM) analysis in 22 active cases,18 inactive cases of SLE and 20 controls.The results showed that:(1)Compared to controls and inactive SLE,apoptosis rates of CD8+ T cells was significantly higher in active SLE group(P0.05),and the expression of Fas in CD8+CD28-T cells and FasL in CD8+CD28+T cells were higher in active SLE group(P0.05).The expression rate of Fas of CD8+CD28+ T cells in SLE patients was featured by the fact that active SLE group inactive SLE groupcontrol group,and there was a significant difference in statistics in these 3 groups(P0.05).(2) The activity of SLE was positively correlated with the apoptosis rate of CD8+T cells,but there was no correlation with expression of Fas、FasL in CD8+CD28-and CD8+CD28+ T cells.(3) The expression of Fas、FasL were correlated with apoptosis,and expression of Fas was significant correlated with expression of FasL in CD8+CD28+ T cells.The expression of Fas was not significantly correlated with expression of FasL in CD8+CD28-Tcells.It is concluded that the apoptotic rate of the peripheral blood CD8+T cells is abnormally increased and the activity of SLE was positively correlated with the apoptotic rate of CD8+T cells.The difference of expression in Fas and FasL between active and inactive cases of SLE may induce the disability of immune tolerance.
Fas ligand
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CD28 interaction with B7 molecules, expressed on the membranes of antigen‐presenting cells, costimulates cytokine production, T‐cell proliferation and generation of cytotoxic lymphocytes. The expression of CD28 markers on CD4 + and CD8 + lymphocytes was studied in a group of subjects at various stages of HIV infection. A reduction in the percentage of CD28‐bearing CD4 + and CD8 + cell subsets was observed during the asymptomatic stage of the disease. This reduction was more pronounced in AIDS than in non‐AIDS patients. At the same time, an increase in the absolute CD8 + CD28 − cell number (greater in stage A than in stage B and C subjects) was observed in HIV‐infected patients. The finding of an altered pattern of CD28 expression on T cells might per se explain certain early defects in the cytokine pattern and in the immune response peculiar to HIV‐infected patients.
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Objective To investigate the changes of T-lymphocyte subsets,tumor necrosis factor(TNF-α) and HLA-DR before and after the treatment of aplastic anemia. Methods 38 cases with aplastic anemia from 2008 to 2010 in our hospital were selected as the study group,while 30 healthy subjects were chosen as the control group. T-lymphocyte subsets and HLA-DR,TNF-α in the two groups were detected by Flow Cytometry(FCM) and ELISA,respectively. Results Before the treatment,the serum CD4+ and CD4+/CD8+ level in the study group were significantly lower than that in the control group(P0.01) ,and the CD8+,HLA-DR and TNF-α level were significantly higher(P0.01) . After the treatment,the CD4+ and CD4+/CD8+ level in the study group increased and the CD8+,HLA-DR and TNF-α level decreased,with statistically significant difference compared with that before the treatment(P0.05) . Conclusion The maladjustment of immune system is closely related to the incidence of aplastic anemia,exibiting disbalance of T-lymphocyte subsets and abnormal activation of T cells.
Aplastic anemia
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Ku70
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Vorinostat
Histone deacetylase inhibitor
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SUMMARY To evaluate whether vertical HIV infection interferes with the expression of CD28 on T lymphocytes, 25 HIV-infected children and 29 seroreverted children born to HIV+ mothers were studied. The percentage of CD28− cells among CD8+ T lymphocytes was higher in HIV-infected children than in controls (P < 0.001). In fact, in HIV-infected children, this percentage was elevated from the first year of life, while in healthy seroreverted children, the proportion of CD28− cells among CD8+ cells rose progressively with age (r = 0.49; P = 0.008). In HIV+ children, the CD8+ CD28−, but not CD8+ CD28+ cell proportion was significantly correlated with immunological markers of disease progression, such as CD4+ cell loss (r = −0.65; P < 0.001) and the level of in vitro spontaneous lymphocyte apoptosis (r = 0.53; P = 0.03).
CD4-CD8 Ratio
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Flip
Lumen (anatomy)
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Flip angle
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