DNA barcoding of Congolese freshwater fish collections
Erik VerheyenF. BremanCélestin Danadu MizaniM. HanssensA. Ibala-ZambaZoltán NagyGontran SonetJeroen Van HoudtEmmanuel VrevenSoleil Lunkayilakio WamuiniJos Snoeks
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Carangidae family has got about 148 species belonging to 32 genera. In Vietnam, Carangidae is of high commercial value and playing an important role in the ecosystem. In the context Vietnam has received yellow card for seafood since Nov. 2017 by the EU, in which one of the main reasons was related to the restriction of traceability. In this study, DNA barcoding technique of mitochondrial cytochrome oxidase I (COI) gene was used to classify 56 specimens of Carangidae from three coastal areas (Northern, Central and Southern) in Vietnam to evaluate the effectiveness compared to the morphological classification method. Results showed that 21 species belonging to 16 genera were determined by the COI barcode while 18 species (16 genera) were determined when using morphological method. Seriola quinqueradiata and Trachinotus anak were newly recorded in Vietnam. From 56 sequences with 660 bp of mtDNA (COI), total 27 haplotypes were detected; haplotype diversity (h) and nucleotide diversity (π) were 0.903 ± 0.00060 and 0.14%, respectively. The DNA barcodes of COI gene of 21 species in Carangidae which were developed in this study could be used as a basis for comparison and traceability of their products. In addition, the results showed the high potentiality in using COI barcode to identify Carangidae fish in Vietnam.
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The present mini-review is related with the recent work carried out in the field of DNA barcoding of different species. The barcoding of marine and freshwater fishes and honey bee samples are mostly studied. The study of DNA barcoding is essential to provide accurate toxinomy, biodiversity of species etc. Highlights of the study is Advances in DNA barcoding of fishes, honeybee. Snake etc are described and also Advantages and disadvantages of DNA barcoding is discussed. K e y w o r d s Biodiversity, freshwater fishes, marine species, DNA barcoding, species identification
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Ghana's waters are biologically diverse with different fish species due to different ecological habitats and niches harboring several cryptic fish species. However, the fish production has reduced over the past decade; hence an urgent conservation and management strategies are required to save Ghanaian fishery such as accurate identification of species to formulate species-specific conservation and management strategies. Molecular method of fish identification, DNA barcoding, has proven its efficacy in species identification for both freshwater and marine species. In recent years, DNA barcoding has been accepted as a bio-identification system for living organisms globally. This system is fast and produces accurate species identification by using a short DNA sequence marker from a standard region of the DNA sequence of an organism's genome to identify it as belonging to a particular individual or species based on Cytochrome C Oxidase type I (COI/ Cox 1) gene instead of the whole genome. Unfortunately, unlike the developed countries, this molecular method of fish identification is new in Ghana. This review article aims to examine the issues regarding fish identification and the need for DNA barcoding as a tool for taxonomic identification, grouping, and naming of fish species in Ghana. Also, this review takes a look at the current status and future direction of DNA barcoding fisheries in Ghana. In addition, the benefits of DNA Barcoding in fishery management and conservation are discussed. Key words: DNA barcoding, Ghana, marine species, current status, management, conservation.
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