[Antibody response to P-fimbriae of Escherichia coli in patients with genitourinary infections].
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The serum antibody response to P-fimbriae of Escherichia coli in patients with genitourinary infections was investigated with enzyme linked immunosorbent assay (ELISA) for P-fimbriae-specific IgG antibody. 1) Female patients with acute pyelonephritis had a significantly (P less than 0.01) higher titer of serum IgG antibody by ELISA, compared with patients with acute cystitis or control subjects. The prevalence of positive serum IgG antibody response was 65% in patients with acute pyelonephritis. 2) Patients with epididymitis with high fever had a significantly (P less than 0.01) higher titer of serum IgG antibody by ELISA, compared with patients with epididymitis without fever or control subjects. The prevalence of positive serum IgG antibody response was 60% in patients with epididymitis with high fever. Therefore, 65% of female patients with acute pyelonephritis and 60% of patients with epididymitis with high fever are infected with P-fimbriated E. coli. The measurement of serum antibody response to P-fimbriae must be helpful for the diagnosis and the antibiotic therapy of epididymitis.Keywords:
Epididymitis
Antibody titer
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The serum antibody response to P-fimbriae of Escherichia coli in patients with genitourinary infections was investigated with enzyme linked immunosorbent assay (ELISA) for P-fimbriae-specific IgG antibody. 1) Female patients with acute pyelonephritis had a significantly (P less than 0.01) higher titer of serum IgG antibody by ELISA, compared with patients with acute cystitis or control subjects. The prevalence of positive serum IgG antibody response was 65% in patients with acute pyelonephritis. 2) Patients with epididymitis with high fever had a significantly (P less than 0.01) higher titer of serum IgG antibody by ELISA, compared with patients with epididymitis without fever or control subjects. The prevalence of positive serum IgG antibody response was 60% in patients with epididymitis with high fever. Therefore, 65% of female patients with acute pyelonephritis and 60% of patients with epididymitis with high fever are infected with P-fimbriated E. coli. The measurement of serum antibody response to P-fimbriae must be helpful for the diagnosis and the antibiotic therapy of epididymitis.
Epididymitis
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Objective:To study the relationship between IgG antibody titer and age and times of pregnancy in pregnant women with blood group O and RhD antigen positive.Method:Irregular antibody and IgG antibody titer were measured in 209 pregnant women with blood group O and RhD antigen positive by microcolumn gel technique.The pregnant women were after 16th weeks of pregnancy and their husbands were with blood group A or B. Result:There were significant differences between groups with different times of pregnancy in IgG antibody titer(in IgG anti-A antibody titerχ2=22.384 3,P0.05,in IgG anti-B antibody titerχ2=24.901 5,P0.05).There were significant differences between different age groups in IgG antibody titer(in IgG anti-A antibody titerχ2=8.343 1, P0.05;in IgG anti-B antibody titerχ2=14.7707,P0.05).Multiple regression analysis showed that age(in IgG anti-A antibody titer OR=2.795,in IgG anti-B antibody titer OR=3.630)and times of pregnancy(in IgG anti-A antibody titer OR=7.945,in IgG anti-B antibody titer OR=6.448)were the risk factors of IgG antibody titer.Conclusion:In order to diagnose and prevent HDN,it would be necessary to detect IgG antibody titer in aged pregnant women or pregnant more than one time with blood group O.
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Anti-thyroglobulin antibody forming cells (AFC) were generated from the cultured peripheral lymphocytes of patients with chronic thyroiditis. In order to clarify whether this reflects the antibody formation in vivo, the number of antibody forming cells generated in the culture was related to the serum antibody titer in 121 cases.There was no case which produced more than 10 AFC from 2×105B-cells in the group with the low serum antibody titer (less than 102), while 29.4% produced more than 10 AFC in the patients with antibody titer of 202 and 204 and 65.2% in the group with titer of 802-6402. The average number of AFC of the last group was 31.4 ± 17.7 (SD).AFC and serum antibody titers were estimated on various occasions with the same patients. A good relationship was observed between these two parameters.There was no significant correlation between the numbers of AFC and serum antimicrosome antibody titers.
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This study on the dynamic change of chicken antibody after inject IgY aims to provide a guidance for clinical use of yolk antibody.50 highland gray cocks whose maternal antibody attenuated to almost disappear randomly assigned into 2 groups.The experiment group were injected with 2 mL IgY including ND-Ab,AVI-Ab H5 and AVI-Ab H9 while the control group with 2 mL Sodium Chloride.The chicken serum antibody titer was measured by hemagglutination inhibition test at different time.Results: the ND-Ab titer was significantly higher than that of control group in 1-8 day(P0.01),AVI-Ab H5 titer was significantly higher than the control group in 1-11 day(P0.01),and AVI-Ab H9 titer was significantly higher than the control group in 1-14 day(P0.01).The serum antibody titers increased significantly just 1d post injection while the high antibody titers continued 5-6 days and then reduced gradually.The half life period of IgY in the serum was about 5days.It can be concluded that injecting the egg yolk antibody can significantly improve chicken serum antibody titer.
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The present work proposed to correlate serum antibody avidity and salivary antibody titers as parameters for time of infection and antigen exposure in a co-hort study evaluating leprosy patients in different periods of treatment. Colorimetric enzyme-immunoassays for salivary antibodies, serum antibody IgG titers and avidities were performed in the samples. Anti-PGL-1 IgA and IgM salivary antibodies were significantly higher in multibacillar (MB-L) patients compared to normal controls (p<0.05), but not when compared to borderline tuberculoid (BT) or to paucibacillar (PB-L) patients (p>0.05). A good correlation was found between salivary anti-PGL-1 IgA and IgM levels in MB-L patients (r=0.41, p<0.01). Two out of 33 tested saliva samples from patients who had completed the drug regimen treatment presented positive salivary antibodies. Among non-treated patients, samples with low, medium or high serum IgG antibody avidity were found in similar frequencies. In patients under treatment, most of the serum samples showed low or medium IgG antibody avidity. The treated MB-L patients showed medium or high antibody avidity, except for two, who showed very low antibody avidity results. We suggest that salivary anti-PGL antibodies and serum IgG avidity could be useful for the indication of recent exposure or re-exposure to bacteria after chemotherapy.
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Through determining the serum antibody and yolk antibody titers regularly in laying hens vaccinated with swine Ecoli (K88) or trivalent (K88, K99, 987P) inactivated vaccines, it was found that the growth decline trends of yolk antibody and serum antibodies were generally similar, but the yolk antibody titers inclined to delay at the beginning of ascending or declining period, and that the average of yolk antibody titers decreased by 218 as compared with serum antibody titers ( P001)
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Objective: To determine the anti-A/B antibody titers of group O red blood cell units(RBC),and to evaluate the safety and feasibility of urgent transfusion of uncrossmatched group O RBC.Methods: Whole blood was collected from 100 volunteer blood donors and fractionated into RBC and plasma units.The anti-A/B antibody titers in whole blood and RBC of 1-,7-and 14-day storage duration were determined and compared.Results: The IgM anti-A/B antibody titers in most of the whole blood samples were between 4 and 128,and the IgG antibody titers between 16 and 256.Correspondingly,the antibody titers were decreased significantly in most RBC(P0.01),in which IgM antibody titers were between 4 and 32,and IgG antibody titers between 8 and 128.However,no significant changes of antibody titers were observed among the 1-,7-and 14-day RBC.Conclusion: The anti-A/B antibody titer in group O RBC was relatively low,which led to the inference that its urgent use without crossmatching assay might be safe.
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Serum samples were collected from 55 pairs of calves that had not passed the first summer and their dams reared in Kagoshima from 1983 through 1985. They were investigated for the HI antibody to Japanese encephalitis virus (JEV) in calves and for the correlation of antibody levels between calves and their dams. The maternally derived antibody was detected in 35 of 55 calves (63.6%), showing the mean antibody titer of 12.4. A significant negative correlation (p<0.01) was noted between the age of calve and titer of passively acquired antibodies. The regression equation suggested that the maternal antibody disappeared from the calf serum at about 3 months of age. On the other hand, 37 of 55 dams (67.3%) were positive for HI antibody to JEV, and the mean titer of the antibody was 12.7. There was a significant correlation (p<0.01) in serum antibody titers between dams and their calves.
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Positive correlation
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Objective: To investigate the regular patterns of antibody production and prepare the antiserum on collagen protein typeⅡ(CⅡ).Methods: The New Zealand white rabbits were immunized with purified chicken and pig CⅡ and then the rabbit sera were collected every 10 days.The improved indirect ELISA method was adopted to detect the titers of anti-CⅡantibodies. Results: When the rabbits were immunized with purified chicken CⅡ,the antibody titers in sera were 1∶3 200 at 10 th day and 1∶204 800 at 20th day.After booster the antibody titers increased to 1∶409 600 at 30 th day and 1∶819 200 at 40 th day.When the rabbits were immunized with purified pig CⅡ,the antibodies could not be detected at 10 th day.But at 20 th day the antibody titer was 1∶3 200.After booster the antibody titers were then increased to 1∶51 200 at 30 th day and 1∶102 400 at 40 th day. Conclusion: The anti-CⅡ antibodies can emerge in serum after latency and the antiserum with high titer can be obtained when the rabbits were immunized with purified chicken and pig CⅡ.The antibody production was consistent with relevant regularity of primary and secondary immune responses.The latent phases and titers of anti-CⅡ antibodies in sera were concerned with collagen proteins from different genus.
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