Biological properties of chloroform/methanol extracts of Coxiella Burnetii.
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Abstract:
Biological properties of antigens of whole cells of C. burnetii in phase I (WCI), their residue (CMR) and extract (CME) obtained by extraction of C. burnetti with chloroform/methanol mixture were subjected to a comparative study. It was shown that CME is able to react specifically with sera containing antibodies against WCI. The inoculation of animals with CME, but not with WCI and CMR does not cause changes of humoral and cell immunity to Coxiella and does not strengthen nonspecific antibacterial activity, but it can stimulate the formation of antibodies. Morphological changes of internal organs of mice were most expressed after the CMR inoculation and were practically absent after the CME inoculation.Keywords:
Coxiella burnetii
Q fever
Rickettsiaceae
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Journal Article Concomitant Human Infection Due to Rickettsia conorii and Coxiella burnetii Get access F. Janbon, F. Janbon Please address requests for reprints to Prof. F. Janbon, Clinique des Maladies Infectieuses A, Centre Médico-Chirurgical Gui de Chauliac, 34059 Mont pellier, France. Search for other works by this author on: Oxford Academic PubMed Google Scholar D. Raoult, D. Raoult Search for other works by this author on: Oxford Academic PubMed Google Scholar J. Reynes, J. Reynes Search for other works by this author on: Oxford Academic PubMed Google Scholar A. Bertrand A. Bertrand Search for other works by this author on: Oxford Academic PubMed Google Scholar The Journal of Infectious Diseases, Volume 160, Issue 2, August 1989, Pages 354–355, https://doi.org/10.1093/infdis/160.2.354 Published: 01 August 1989
Rickettsia conorii
Coxiella burnetii
Q fever
Concomitant
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Biological properties of antigens of whole cells of C. burnetii in phase I (WCI), their residue (CMR) and extract (CME) obtained by extraction of C. burnetti with chloroform/methanol mixture were subjected to a comparative study. It was shown that CME is able to react specifically with sera containing antibodies against WCI. The inoculation of animals with CME, but not with WCI and CMR does not cause changes of humoral and cell immunity to Coxiella and does not strengthen nonspecific antibacterial activity, but it can stimulate the formation of antibodies. Morphological changes of internal organs of mice were most expressed after the CMR inoculation and were practically absent after the CME inoculation.
Coxiella burnetii
Q fever
Rickettsiaceae
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Isolates of Coxiella burnetii from different geographic regions in Europe, USA, Japan and Africa were compared in their binding properties to the monoclonal antibody (MoAb) 1/4/H directed against the lipopolysaccharide (LPS) of C. burnetii strain Priscilla. Immunoblot analysis and enzyme-linked immunosorbent assay (ELISA) revealed different binding patterns of C. burnetii isolates under study. Most of the isolates tested did react with MoAb 1/4/H. Only four of 20 groups of isolates and one isolate of an otherwise positively reacting group did not react with MoAb 1/4/H. The results indicate a significant variation of LPS structure of the C. burnetii isolates studied.
Coxiella burnetii
Q fever
Rickettsiaceae
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Coxiella burnetii
Q fever
Isolation
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Hares (Lepus europaeus L.) infected subcutaneously (s.c.) or per os with Coxiella burnetii and s.c. with Rickettsia slovaca overcame an inapparent infection accompanied by irregular rickettsaemia and distribution of rickettsiae to different organs and variable antibody response. Neither riskettsia could be detected in faeces of infected animals, but C. burnetii was found in the urine of one hare, which died on day 13 post infection (p.i.). Antibodies against C. burnetii persisted for one year of observation period, those against R. slovaca up to the 181st day p.i. Based on the results obtained and data on other lagomorph species, the hare could play an important role as host of C. burnetii and R. slovaca in nature.
Coxiella burnetii
Rickettsiaceae
Q fever
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Acute Q fever is an emergent and severe disease in French Guiana. We obtained 5 Coxiella burnetii isolates from samples of patients from Cayenne and found an epidemic clone circulating in Cayenne. This clone has caused pneumonia and endocarditis and seems to be more virulent than previously described strains.
Coxiella burnetii
Q fever
clone (Java method)
Rickettsiaceae
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Three hundred and twenty-five sera from blood donors in the south of France were examined by means of the indirect fluorescent antibody test. 18% of the sera had antibodies to Rickettsia conorii, with a significantly higher prevalence (26%) in urban and suburban areas than in semi-rural areas (13 to 16%). This supports the view that in the south of France the highest prevalence of Mediterranean Spotted Fever is suburban. 5% of the sera had antibodies to Coxiella burnetti in this area, in which Q fever is endemic.
Rickettsia conorii
Coxiella burnetii
Q fever
Boutonneuse fever
Rickettsiaceae
Rickettsia typhi
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A competitive enzyme immunoassay (CEIA) was established and compared with other serological techniques for detecting Coxiella burnetii antibody in camels, goats, and sheep. This technique was evaluated because a conjugated anti-camel immunoglobulin was not available to serve as a direct signal for the demonstration of antigen-antibody reaction. A C. burnetii antibody-positive human serum and a peroxidase-conjugated anti-human immunoglobulin G were used as an indicator system competing against antibody in animal serum or as an indicator of the absence of antibody. Sera were considered antibody positive when the A414 of the test sera plus the competing positive antibody was less than or equal to 50% of the A414 of the negative-control serum plus the competing antibody. Antibody to C. burnetii was repeatedly demonstrated in 66% of camel serum samples (n = 200) by the CEIA. Among 48 camel serum samples, 71% were positive for antibody by CEIA versus 65% by EIA using peroxidase-labeled protein A. The CEIA detected C. burnetii antibody in 63% of sheep serum samples (n = 40) and in 50% of goat serum samples (n = 96), while the indirect fluorescent-antibody technique detected antibody in 38% of sheep and 34% of goat serum samples and the EIA detected antibody in 50% of sheep and 35% of goat serum samples. These data indicate that the CEIA is a reliable and sensitive technique for demonstrating C. burnetii antibody in camels, sheep, and goats.
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Rickettsiaceae
Q fever
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An enzyme-linked immunosorbent assay (ELISA) was developed to detect immunoglobulin G to Coxiella burnetii phase II. Serum samples from 213 patients who had had Q fever 1 year previously and from 301 blood donors from six localities in Switzerland were tested by ELISA and by indirect fluorescent-antibody (IFA) and complement fixation (CF) tests. The ELISA and the IFA and CF tests detected antibody to C. burnetii in 202 (94.8%), 193 (90.6%), and 166 (77.8%) of the 213 Q fever patients, respectively. With the serum samples from blood donors, the ELISA yielded a higher percentage of positive sera than did the IFA and CF tests. The high specificity of the three tests was confirmed by analyzing paired serum samples from 36 patients suffering from acute pneumonia of viral or bacterial origin. In these cases, the serological results were negative by the three tests, except for three Q-fever cases included as positive control.
Coxiella burnetii
Complement fixation test
Q fever
Immunoglobulin M
Rickettsiaceae
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Q fever is known to be a worldwide disease, with Sweden supposed to be one of a few exceptions. The purpose of this pilot study was to elucidate whether or not a potential risk group for obtaining Q fever in Sweden was seropositive to the causative agent Coxiella burnetii. Blood samples were collected from sheep farmers on the island of Gotland, and from members of their families. Serum samples were examined by ELISA for the presence of antibodies against C. burnetii, phases I and II. Positive reactions were confirmed with Western blot analysis. It was found that 30% of the study group were seropositive to C. burnetii, thus indicating that Q fever is endemic in this area of Sweden.
Q fever
Coxiella burnetii
Rickettsiaceae
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