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    Effect of nitrogen application on nitrogen uptaking and utilization in ten different rice varieties
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    Abstract:
    Using ten rice varieties of different nitrogen dry matter production efficiency as materials,the effects of four nitrogen treatments on nitrogen dry matter production efficiency(NDMPE),dry matter accumulation(DMA) and nitrogen accumulation(NA) were investigated in pot experiments.The results showed that the nitrogen application increased DMA and NA,but different genotype rice had different increment.With the nitrogen application,the NA and nitrogen content(NC) and DMA increased significantly,but the DMPE obviously declined.The NA was significantly correlated with NC and DMA,the NDMPE was significantly negatively correlated with NA and NC.Significant difference of soil nitrogen apparent balance appeared among different rice varieties,and the difference become more obvious with the increase of nitrogen application.
    In this micro-plot experiment,sugarcane varieties ROC22 was used as experimental materials,and three nitrogen levels were desiged with 0.75 g,1.5 g and 2.25 g 15N-labeled urea/pot at sowing time respectively,to study the influences of nitrogen levels on absorption and distribution N,P,K of sugarcane in early growth stage.The results showed that 15.18%~16.77% nitrogen of sugarcane accumulation was derived from nitrogen fertilizer,and 83.23%~84.82% was from soil and seed-stem.The nitrogen utilization rate of sugarcane was 1.76%~3.44%.The dry matter accumulation,nitrogen accumulation,phosphorus accumulation,potassium accumulation and the proportion of N from nitrogen fertilizer in sugarcane plant showed a increasing trend with the increase of nitrogen fertilizer,while nitrogen utilization rate of sugarcane decreased significantly.N showed a synergy relationship with P and K.The nitrogen levels had influenced on N,P and K,especially fertilizer-nitrogen distribution in sugarcane roots.
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    Three soybean varieties,which were widely planted in Heilongjiang province,were planted in pots with the same amount of N and K fertilizer,but four different levels of phosphorus.The nitrogen amounts in different organs were determined with the classical method of Kai's Fixed Nitrogen.The results suggested that there were relatively large effects of phosphorus on the nitrogen accumulation in total plants and each organ.In all three soybean varieties with different phosphorus treatments,the nitrogen amounts in total plants were gradually increased from branching stage and reached peak values at ripening stage.Suitable phosphorus level showed beneficial for the accumulation of nitrogen.The highest accumulation of nitrogen in total plants were obtained with P_5 treatment after flowering stage,indicating that suitable phosphorus application can help nitrogen reaching optimum balance,acquiring higher accumulation level.Nitrogen accumulation was higher in the variety with high protein than that in the variety with medium level of protein and high level of oil content,suggesting the nitrogen requirements was higher in high protein content variety.The higher nitrogen amounts in plants was followed by higher yield of single plant and higher content of protein,whereas fat content was lower.The nitrogen had little effect on fat formation.
    Nitrogen deficiency
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    Potassium, nitrogen and their interaction play important roles in plant growth, development and yield of rice. Appropriate potassium can stimulate nitrogen accumulation and translocation in rice. In order to identify plant nitrogen absorption, distribution and utilization characteristics of rice under potassium supply, a field experiment was conducted with four potassium rates (0, 90, 180, 270 kg ha-1 K2O) using conventional japonica cultivars (Wuyunjing 7 and Wuxiangjing 14) and hybrid japonica cultivars (86 you 8, Siyou 422) from 2004 to 2005 on Experimental Farm of Nanjing Agricultural University, Jiangsu province, China. Potassium application increased rice yield (4.56?14.77%) and nitrogen accumulation during growth stages, with the maximum from elongation stage to heading stage, but nitrogen uptake ratio decreased. Potassium application enhanced nitrogen transport amount and percentage after anthesis, as well as nitrogen accumulation in different rice organs, nitrogen distribution percentage in leaves and panicles, whereas declined nitrogen distribution percentage in culm and sheath. Nitrogen absorption from fertilizer, basic nitrogen recovery efficiency before elongation stage, nitrogen recovery efficiency during the whole growing stage, and nitrogen harvest index were raised significantly, but plant nitrogen productivity was opposite. Grain yield, nitrogen accumula- tion, nitrogen translocation amount and percentage after anthesis, and nitrogen distribution in different rice organs amounted to the maximum at K2O application rate of 180 kg ha-1, as well as nitrogen absorption from fertilizer, basic nitrogen recovery efficiency before elongation stage, nitrogen recovery efficiency during the whole growing stage. However, nitrogen absorption percentage from elongation stage to heading stage, nitrogen distribution percentage in culm and sheath, plant nitrogen productivity decreased to the minimum. The yield and nitrogen recovery efficiency of conventional japonica cultivars were higher than those of hybrid japonica cultivars.
    Anthesis
    Panicle
    Elongation
    Nitrogen deficiency
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    Field experiments were conducted in a randomized complete block design with three nitrogen fertilize rate(NH,High nitrogen:210 kgN.ha-1;NM,Middle nitrogen: 150 kg N.ha-1;NL,Low nitrogen: 90 kg N.ha-1) and two indica high quality rice genotypes(Baguixiang and Guihuazhan) to investigate dry matter accumulation and translocation and grain growth after flowering in 2007 and 2009.Results showed that:(i)Dry matter translocation efficiencies ranged from 53.60 % to 62.23 % and nitrogen translocation efficiencies were from 12.33 % to 37.95 %,and dry matter rate contribution of culm and sheath and leaf blade to grain were from 12.33 % to 37.95 %,and nitrogen rate contribution of culm and sheath and leaf blade to grain were 47.93 % to 117.2 % under different nitrogen levels.(ii) Nitrogen application levels made influence on carbon and nitrogen remobilization and grain growth after flowering of high quality indica of Baguixiang and Guihuazhan.Carbon nitrogen remobilization in culm,sheath,leaf blade were higher under high nitrogen fertilizer application than middle and low N level,while it was not easy to remobilize carbon and nitrogen in culm and sheath to grain,and reduced translocation efficiencies of starch of culm and sheath.Carbon and nitrogen accumulation,weight of panicle,protein content and nitrogen harvest index of grain increased with more nitrogen fertilization.Although low-nitrogen can promote translocation efficiencies of the assimilation of carbon and nitrogen in culm and sheath,but the grain harvest index was significantly lower.(iii) Under different nitrogen levels,grain growth was closely correlated with carbon and nitrogen remobilization.Initial grain filling potential was correlated with dry matter remobilization rate and efficiency of culm and sheath.Maximum grain filling rate was correlated with leaf dry matter remobilization rate and efficiency after flowering.This correlation displayed was positive correlation between the total nitrogen transportation rate of leaf blade and the grain protein yield with more nitrogen application.Grain protein and starch content and accumulation of high-quality rice were more correlated with the translocation rate of total nitrogen in leaves with increasing nitrogen application.The stem and leaf nitrogen accumulation reduced along with the increase in grain nitrogen accumulation and grain protein content increased in synchronization.The C/N ratio of carbon and nitrogen assimilation of culm and sheath after anthesis was positively correlated with protein content and yield and negative correlated with amylose content and ratio of starch and protein.It can be concluded that differences among each N level in nitrogen translocation efficiencies and nitrogen rate contribution depended on the nitrogen utilization and partition agronomic characteristics.
    Panicle
    Anthesis
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    In two growing seasons of wheat (2015-2017), we conducted a field trial with Taishan 28 in Tai'an Academy of Agricultural Science Feicheng experimental base, Tai'an City, Shandong Province. There were four irrigation levels of 150 (A1), 300 (A2), 450 (A3), and 600 (A4) m3·hm-2, and four nitrogen application levels of 90 (B1), 135 (B2), 180 (B3), and 225 (B4) kg·hm-2. We examined the effects of the combination effects of irrigation and nitrogen on dry matter accumulation and transport, nitrogen accumulation and transport, water consumption and utilization, photosynthetic characteristics, wheat grain yield and yield components of wheat. The results showed that dry matter accumulation, nitrogen accumulation, vegetative organs production, storage and the transportation volume to grains of the dry matter and nitrogen, and dry matter and nitrogen accumulation of grain in the mature stage of wheat all reached the maximum in A3B3 treatment, which were significantly different from other treatments. Under all the nitrogen treatments, soil water consumption in the 60-200 cm soil layer was A3>A4>A2>A1. Water use efficiency and nitrogen use efficiency in A3B3 treatment were higher than that under A3B4, A4B3 and A4B4. The net photosynthetic rate, stomatal conductance and transpiration rate of flag leaves from 7 to 28 days after flowe-ring were all significantly higher in A3B3 treatment, which was conducive to the photosynthetic synthesis of carbohydrates in wheat. The interaction effect of water and nitrogen addition significantly affected grain yield and yield components. Wheat yield was the highest in A3B3 treatment which reached at 9400 kg·hm-2. In conclusion, the treatment with irrigation of 450 m3·hm-2 and nitrogen of 180 kg·hm-2 could significantly improve dry matter and nitrogen accumulation, and promote transportation volume of the dry matter and nitrogen to grain. Compared with the high water and nitrogen treatment, it could effectively increase water use efficiency and nitrogen use efficiency, enhance photosynthetic capacity of flag leaf, produce more carbohydrate, and increase grain yield.
    Stomatal Conductance
    In order to understand the response of some physiological characteristics and yield to nitrogen levels in soybean varieties with different nitrogen photosynthetic efficiency,the study on the root activity,nitrate reductase activity,nitrogen content of leaf,chlorophyll content,net photosynthetic rate,dry weight of single plant and yield of two high-level and two low-level nitrogen photosynthetic efficiency varieties under five nitrogen levels from R2 to R6 stage was conducted.The results showed that the change in physiological characteristics and yield of four soybean varieties showed a single peak curve which were increased at the beginning and then decreased with the nitrogen levels.The comparisons of different nitrogen photosynthetic efficiency varieties released in similar years showed that the root activity,nitrate reductase activity,net photosynthetic rate,dry weight of soybean varieties with high level nitrogen photosynthetic efficiency was higher than low level varieties at low nitrogen(40-80 kg·ha-1)treatment,but it was lower than the low level varieties at high nitrogen(120-160 kg·ha-1)treatment,the differences reached extremely significant level(P0.01)at R6 stage,and they were significant(P0.05)or non-significant at R2 stage and R4 stage.Total nitrogen content and chlorophyll content in leaves of varieties with high level of nitrogen photosynthetic efficiency were significantly lower than the low level in five nitrogen levels.So the medium and low amount of nitrogen fertilizer should be applied for varieties with high nitrogen use efficiency,similarly,increasing the corresponding amount of fertilizer to apply for varieties with low nitrogen use efficiency was feasible.
    Dry weight
    Nitrogen deficiency
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    Nitrogen application rates of 0,75,150,225,300 and 375 kg·hm-2 on dry matter accumulation and distribution as well as the N,P,K uptake and utilization of hybrid cotton were studied under high yield conditions.The results showed that the nitrogen utilization and dry matter content were significantly positive correlated with N,P,K accumulation.The nitrogen can improve the dry matter and N,P,K accumulation.But the effect that applying nitrogen was no longer significant when the nitrogen application rate increased to 300 kg·hm-2.The analysis of nitrogen content on dry matter accumulation of different organs indicated that nitrogen content was positive correlated with leaves and negative correlated with stems in the middle stage of cotton growth periods and positive correlated with stems in the latter growth stage.The nitrogen content was positively correlated with cotton bud and flower in the middle growth stage while negative in the latter growth stage.When the nitrogen application rate increased to 300 kg·hm2,nitrogen decreased the proportion of N,P,K and dry matter distributed to reproductive organs and increased the proportion of leaves and stems.The vegetative growth of the hybrid cotton was excessively vigorous.In this experiment,applying the nitrogen of 300 kg·hm-2 had the highest seed cotton yield which was 1.66% higher than the nitrogen of 225 kg·hm-2,but there was no significant difference.When nitrogen rate increased to 375 kg·hm-2,the seed cotton yield decreased by 2.23% and 3.92% compared with N 300 kg·hm-2 and 225 kg·hm-2,respectively.The nitrogen utilization decreased distinctly while the phosphorus and potassium utilization increased with the more nitrogen fertilizer added.
    Nitrogen deficiency
    Citations (2)
    Under the condition of different proportion of three nitrogen amounts with three potassium amounts,the effects of different proportions of nitrogen and potassium amounts on the nitrogen absorption and utilization were discussed. The result indicated: the total accumulation and utilization of nitrogen were the highest in the N 2 K 3 treatment. At the same nitrogen level,the nitrogen accumulation increased with potassium fertilizer amount,there were significant differences among different treatments. The nitrogen accumulation showed no significant difference when the nitrogen amount was 165 kg / hm2and 210 kg / hm2at the same level of potassium fertilization. The increase of nitrogen and potassium amounts was conducive to raising nitrogen accumulation before the panicle differentiation stage of direct-seeded rice. The increase of nitrogen and potassium amounts was conducive to raising the nitrogen distribution proportions in vegetative organs,and nitrogen distribution proportion in panicle decreased with increase of amount of potassium applied. The nitrogen translocation rate and contribution rate of each organ nitrogen to grain increased with the increase of nitrogen fertilizer amount. The nitrogen transport rate of leaf and sheath declined with increased amount of potassium fertilizer at the level of nitrogen fertilizer of 120 kg / hm2and 165 kg / hm2. Potassium fertilizer had obvious influence on the nitrogen translocation rate at the level of nitrogen amount of 210 kg / hm2. The activites of NR,GOGAT,GS increased with nitrogen fertilization before heading. The activity of nitrogen metabolism enzyme of N 2 treatment was the highest usually after heading. The activites of NR,GOGAT,GS increased with potassium fertilizer. The nitrogen agronomy utilization ratio of N 2 was the highest at three nitrogen fertilizer levels. Increase of potassium fertilizer amount improved the utilization rate of nitrogen with the same amount of nitrogen fertilization,this effect was significant at low nitrogen levels. The increase of fertilization amount of nitrogen and potassium decreased the harvest index of nitrogen. 165 kg / hm2of nitrogen and 210 kg / hm2of potassium made the best coordination group for cultivation of direct-seeded rice.
    Panicle
    Nitrogen deficiency
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    Polyethylene glycol( PEG-6000) was used in this study to simulate water stress and comparatively analyze the differences in physiological index and agronomic traits of two different nitrogen use efficiency hybrid rice varieties( N-efficient species De xiang4103 and N-inefficient species Yi xiang 3724) under the conditions of different nitrogen concentrations( N0,N1,N2 respectively 0,2. 5,5 mmol / L) and different water stress( P0,P1,P2,respectively 0,5 %,10 %) conditions at the seeding stage. The results showed that the physiological index and agronomic traits of all varieties in no nitrogen levels were significantly lower than that of nitrogen levels,and with the increase degree of water stress,the two species of photosynthetic pigments,soluble sugar,proline,nitrogen accumulation,seedling height and Seedling thickness showed a decreasing trend. Under the nitrogen fertilizer condition,with the increase degree of water stress,to De Xiang 4103,the content of photosynthetic pigment,soluble sugar increased( In addition to N2 level decrease),the proline content first decreased and then increased,seedling height and Seedling thickness decreased,the accumulation of dry matter and nitrogen decreased in N1 level and decreased at first and then increased in N2 level; to Yi xiang 3724,Soluble sugar increased( In addition to N1 level decrease),the accumulation of dry matter and nitrogen decreased at first and then increased,the remaining indicators behaved the same with De Xiang 4103. After rehabilitation treatment,the indicators showed different degrees of increased or decreased,that is the different compensation effects; and from the water stress treatment to after rehabilitation treatment,the accumulation of total nitrogen and dry matter are positively related to certain accumulation in two rice varieties. It can be seen that different nitrogen species showed different reactions to different water and nitrogen efficiency,and different degrees of synergistic effect of water and nitrogen. N-efficient species De xiang 4103 had stronger ability to adapt nitrogen and water stress than N-inefficient species Yi xiang 3724.
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