[Repairing segmental radial bone defect with poly (3-hydroxybutyrate-co-3-hydroxyvalerate)/sol-gel bioactive glass composite porous scaffold].
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To investigate the capability of the bone regeneration of poly (3-hydroxybutyrate-co-3-hydroxyvalerate/sol-gel bioactive glass (PHBV/SGBG) composite porous scaffold.PHBV/ SGBG composite porous scaffold was implanted into the segmental radial bone defect of the New Zealand white rabbits, PHBV/hydroxylapatite (PHBV/HA) as experimental control. The degradability, biocompatibility, and bone regeneration capability of the implants were evaluated through radiological, histological, computerized graphic, and biomechanical analysis.The new bone formation occurred as early as 4 weeks after implantation of PHBV/SGBG composite porous scaffold. The defect was filled with new bone 8 weeks after the implantation, and was completely repaired 12 weeks after operation. The new bone had normal bone structure and the medullar cavity regenerated. The biomechanical study showed that the anti-compression force of radial specimen in PHBV/SGBG groups was significantly higher than in PHBV/ HA groups (P < 0.05), but no significant difference existed between PHBV/SGBG group and autograft bone group (P>0.05). The PHBV/SGBG composite porous scaffold degraded no sooner than 4 weeks after the implantation and most of scaffold was absorbed after 12 weeks. The proportion of the scaffold to new bone decreased from 60% by week 4 to 8% by week 12.PHBV/SGBG composite porous scaffold is a degradable bone substitute. It can achieve early bone generation and complete repair. It can be used as an ideal scaffold for tissue-engineering bone.Keywords:
Biocompatibility
Bioactive Glass
Hydroxylapatite
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To investigate the capability of the bone regeneration of poly (3-hydroxybutyrate-co-3-hydroxyvalerate/sol-gel bioactive glass (PHBV/SGBG) composite porous scaffold.PHBV/ SGBG composite porous scaffold was implanted into the segmental radial bone defect of the New Zealand white rabbits, PHBV/hydroxylapatite (PHBV/HA) as experimental control. The degradability, biocompatibility, and bone regeneration capability of the implants were evaluated through radiological, histological, computerized graphic, and biomechanical analysis.The new bone formation occurred as early as 4 weeks after implantation of PHBV/SGBG composite porous scaffold. The defect was filled with new bone 8 weeks after the implantation, and was completely repaired 12 weeks after operation. The new bone had normal bone structure and the medullar cavity regenerated. The biomechanical study showed that the anti-compression force of radial specimen in PHBV/SGBG groups was significantly higher than in PHBV/ HA groups (P < 0.05), but no significant difference existed between PHBV/SGBG group and autograft bone group (P>0.05). The PHBV/SGBG composite porous scaffold degraded no sooner than 4 weeks after the implantation and most of scaffold was absorbed after 12 weeks. The proportion of the scaffold to new bone decreased from 60% by week 4 to 8% by week 12.PHBV/SGBG composite porous scaffold is a degradable bone substitute. It can achieve early bone generation and complete repair. It can be used as an ideal scaffold for tissue-engineering bone.
Biocompatibility
Bioactive Glass
Hydroxylapatite
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Nano hydroxyapatite(n-HA) and its composite with poly(e-caprolactone)(PCL) were fabricated by hydrothermally synthesized method,and the well interconnected macroporous nano-composite scaffolds with pore diameter from 200μm to 400μm were prepared by melt-cast/salt particle-leaching technique.The biological properties of the composite scaffolds were investigated through cell culture and animal implanted experimentation.The results show that porosity of the composite scaffolds increases with the increase of the quantity of the porogens used while the compressive strength decrease.The maximal porosity of the composite scaffold can reach 86% while its compressive strength is only 2.4MPa.The attachment ratio and proliferation of MG63 cells on the composite scaffolds increase with the increase of HA content in the composite,which are significantly higher than those of PCL alone.Histological examinations confirm that the new bony tissue could grow easily into the composite scaffold and directly integrate with the composite by bone-bonding.The results indicate that the n-HA/PCL composite scaffolds have excellent biocompatibility and bioactivity.
Biocompatibility
Caprolactone
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Volume fraction
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By using the active filler controlled polymer pyrolysis, new and cost-effective composite materials can be obtained. In this work, ceramic matrix composites were prepared by using this precursor route, using a polysiloxane network filled with metallic niobium and aluminum powders as active fillers. The mixtures were blended, uniaxially warm pressed, and pyrolyzed in flowing argon at 1400 °C. Porous ceramic preforms were infiltrated with a LZSA glass material, in order to improve the density of a porous composite material. The properties of the pyrolyzed composite material and the effect of the LZSA infiltration on the Al2O3-NbC-SiOC ceramic composite material were investigated. The results have showed that the infiltration processes has improved the physical and mechanical properties of the composite material.
Ceramic matrix composite
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Biomimetic scaffold for bone tissue engineering was prepared by mixing Sol-Gel bioactive glass with type I collagen through freeze-drying technique.In vitro,the biocompatibility of the scaffold was inves-tigated by observing the adhesive,proliferative and differential behaviors of the rat mesenchymal stem cells(rMSCs).In vivo,the composite scaffold seeded with osteoblasts was implanted subcutaneously into the im-munodeficient mice for 6w.It was proved that the composite scaffold was non-cytotoxic and suitable for cells’ proliferation,which was confirmed by the increase of double stranded DNA(ds DNA).The differentia-tion of rMSCs on the composite scaffold was also observed by positive expressions of alkaline phosphatase(ALP) and osteocalcin after osteoinduction for 14d.The results of general and histological observation showed that cells successfully spread on the surface and migrated into the interior of the scaffold.Moreover,bone formation analysis of cell-scaffold constructs in vivo showed that bone tissue and blood vessels were re-generated both inside and on the border of the scaffold-stack.All results demonstrate the Sol-Gel bioactive glass-type I collagen scaffold with good biocompatibility and osteogenesis is a new ideal scaffold for bone tissue repair and regeneration.
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Bioactive Glass
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Objective: To develop a novel scaffold for nerve regeneration and explore its biocompatibility through culturing Schwann cells (SCs) on it an in vivo implantation.Methods: A novel scaffold of PDLLA with arginine-glycine-aspartic acid (RGD) peptide (PRGD/PDLLA) was synthesized.The proliferation of Schwann cells (SCs) concultured with PDLLA films (control group) and PRGD/PDLLA films were evaluated by MTT assay and scanning electron microscope observation.Then, histological assessment and ELISA testing on inflammatory-related cytokines TGF-β1 were performed.Results: compared with PDLLA, PRGD/PDLLA films possesses better hydrophilicity ， biocompatibility, degradation property and less inflammatory reaction.Conclusion: The present study indicated that PRGD/PDLLA scaffolds would meet the requirements of artificial nerve scaffold and have a potential application in the fields of nerve regeneration.
Biocompatibility
MTT assay
Nerve guidance conduit
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Bioactive Glass
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Abstract Repair of critical bone defects is a challenge in the orthopedic clinic. 3D printing is an advanced personalized manufacturing technology that can accurately shape internal structures and external contours. In this study, the composite scaffolds of polylactic acid (PLA) and nano-hydroxyapatite (n-HA) were manufactured by the fused deposition modeling (FDM) technique. Equal mass PLA and n-HA were uniformly mixed to simulate the organic and inorganic phases of natural bone. The suitability of the composite scaffolds was evaluated by material characterization, mechanical property, and in vitro biocompatibility, and the osteogenesis induction in vitro was further tested. Finally, the printed scaffold was implanted into the rabbit femoral defect model to evaluate the osteogenic ability in vivo . The results showed that the composite scaffold had sufficient mechanical strength, appropriate pore size, and biocompatibility. Most importantly, the osteogenic induction performance of the composite scaffold was significantly better than that of the pure PLA scaffold. In conclusion, the PLA/n-HA scaffold is a promising composite biomaterial for bone defect repair and has excellent clinical transformation potential.
Biocompatibility
Biomaterial
Polylactic Acid
Bone tissue
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Asymmetric chitosan scaffold with a loose layer and a dense layer exhibited outstanding bone regenerative ability and appropriate degradability.
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