Correlative study between CT features and expression of N-ras gene、C-myc gene in hepatocelluar carcinoma
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Objective:To study the relationship between SCT findings and the expression of N ras gene?C myc gene in hepatocellular carcinoma(HCC).Methods:SCT scan was performed in 36 patients with pathologically proved hepatocellular carcinoma,and the expression of N ras?C myc gene in tumor specimens was estimated with immunohistochemical SABC method.Results:C myc protein positive rate was higher in the group whose diameter is smaller than 5cm and equal to 5cm,and larger than 3cm than that whose diameter is smaller than 3cm and equal to 3cm,and lower in the group cirrhosis of liver(P0.05).Conclusion:This study shows the SCT findings of HCC is related with the expression of the gene,and the SCT imaging of HCC can indicate the expression of C myc gene to some extent.Cite
Objective To investigate expression of P16 and P21 proteins in hepatocellular carcinoma (HCC) and its relationship to clinicopathological parameters. Methods Expression of P16 and P21 proteins were examined in 46 HCCs and their 38 pericarcinomatous tissues with immunohistochemical strepto avidin biotin complex method. The correlation between P16, P21 protein expression and clinicopathological parameters in HCC was analyzed. Results The deletion rate of P16 protein expression was significantly higher in carcinomatous tissues (58.7%) than in their pericarcinomatous tissues(34.2%)( P 0.05);in Edmondson grade Ⅲ Ⅳ HCC(78.9%)than in Edmondson gradeⅠ andⅡ HCC (44.4%) ( P 0.05); in 3cm HCC (65.0%) than in≤3cm early HCC (16.7%)( P 0.01)in diameter. However, no significant difference was observed between the HCCs with and without metastasis in portal vein. The deletion rate of P21 protein expression was also significantly higher in carcinomatous tissues (80.4%) than in their pericarcinomatous tissues(47.4%)( P 0.01). But, no significant difference was seen between the two groups with different tumor differentiation, different tumor diameters, with and without metastasis in portal vein. Conclusion The deletion of P16 protein expression is closely related to differentiation, invasion and deterioration of HCC.
HCCS
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Objective: To investigate the expression and clinical significance of gene of phosphate and tension homology deleted on chromsome ten( PTEN) and p53 in hepatocellular carcinoma( HCC) sample. Methods: The protein levels of PTEN and p53 were examined by EliVision immunohistochemistry in 70 samples of HCC tissue and 40 samples of adjacent hepatic tissue,while the correlation between the expression and clinicopathological features was analyzed. Results: The positive expression rate of PTEN in HCC tissue was 57. 1%,significantly lower than that in adjacent hepatic tissue,which the positive expression rate was 95. 0%( P 0. 01). Patients with advanced stage of tumor or lymph node metastasis had a lower tendency to express PTEN in HCC tissue( P 0. 01). The positive expression rate of p53 was 60. 0% in HCC tissue,significantly higher than that in adjacent hepatic tissue,which the positive expression rate was 15. 0%( P 0. 01). There were no statistical significance for the p53 expression in HCC tissue in clinicopathological parameters such as tumor size,pathologic grade,TNM stage and lymphnode metastasis( P 0. 05). The expression of PTEN was negatively related with that of p53 in HCC tissue( P 0. 05). Conclusions: The expression of PTEN may be associated with the progression of HCC. It may serve as an index for prognosis of HCC.
Clinical Significance
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Objective:To study the status and significances of bcl 2,p53 and p21 gene expression in hepatocellular carcinoma(HCC).Methods:The samples resected from 55 cases of hepatocellular carcinoma and nodular liver cirrhosis were detected with the antibodies against bcl 2,p53 and p21 in the S P immunohistochemistry study.The relationships between the expression rate and pathological grades and prognosis were analyzed.Results:The positive expression rate of bcl 2 gene protein in HCC and nodular liver cirrhosis were 72.7%(40/55) and 36.4%(20/55) respectively,both present disparity( P 0.05).The positive expression rate of p53 protein in HCC was 54.5%(30/55),the negative expression of all nodular liver cirrhosis tissues was found,both present striking disparity ( P 0.01).The positive expression rate of p21 protein in the HCC and nodular liver cirrhosis were 63.6%(35/55) and 38.2% (21/55) respectively,both not present disparity ( P 0.05).The expression rate and expression intensity of bcl 2、p53 and p21 was not associated with pathological grades in the HCC ( P 0.05). In addition,together expression rate of bcl 2、p53 and p21 in the follow up patients (16 cases) was 50%(8/16).Conclusion:The results suggest that the occurrence of HCC has a close relation with the over expression and mutation of bcl 2、p53 and p21 gene proteins.There may be a joint action among these genes.Because the expression of bcl 2、p53 and p21 can reflect the differential and proliferative degrees of tumour cells,it can be used as the indicators in the diagnosis and prognosis of HCC.
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Objective To investigate the expression of survivin and its relationship with the clinicopathologic feature of hepatocellular carcinoma(HCC),and the correlation between the expression of survivin and c-myc.Methods In 59 specimens of HCC tissues and non-cancerous adjacent liver tissues,the expression of survivin and c-myc was detected by using immunhischemistry and Western-blotting.Results Of the 36(61.0%) cases of HCC expression survivin in 59 cases of HCC,29 were positive for the expression of c-myc and 7 were slightly positive or negative;of the 23 cases without expressing survivin or slightly positive,5 cases were positive for c-myc and 18 negative or slightly positive.Survivin was not detected in non-cancerous adjacent liver tissues.The expression of survivin in HCC had no association with tumor size,capsule status,the classification of cirrhosis and TNM stage(P0.05),but with portal vein tumor thrombus and local recurrence(P0.05). The expression of survivin was significantly correlated with the expression of c-myc in HCC(P0.05).Conclusion Survivin probably promotes the metastasis of HCC by enhancing the function of c-myc,and survivin could be a new diagnostic/therapeutic target in HCC.
Survivin
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Objective To investigate the expression level of AEG-1, β-catenin and C-myc in primary hepatocellular carcinoma. Methods 40 cases of primary hepatocellular carcinoma tissue were used as study samples. The clinical and pathological features of liver cancer patients were recorded. The expression level of AEG-1,β-catenin and C-myc were determined by using immunohistochemistry and Western Blot method.Results The expression rate of AEG-1,β-catenin protein and C-myc in primary hepatocellular carcinoma tissues were 72. 5%,75. 0% and 62. 5%; the AOD value score were 0. 80 ± 0. 07,0. 75 ± 0. 06 and 0. 69 ±0. 06; and the relative expression level was 1. 56 ± 0. 12,1. 23 ± 0. 11 and 1. 15 ± 0. 09,respectively. The expression level of three protein were correlated with pathologic stage differentiation and TNM stage( P 0. 05).There was no significant correlation with patient 's gender,age,tumor size,HBs Ag,and preoperative AFP concentration( P 0. 05). Conclusion AEG-1,β-catenin and C-myc might become guidance indexes for malignant hepatocellular carcinoma evaluation,and can be used to assess disease progression and prognosis.The role of AEG-1,β-catenin and C-myc protein in the original recurrent hepatocellular carcinoma onset process pathway may be independent from AFP system.
Clinical Significance
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Objective To explore the expression and significance of amplified-in-breast cancer 1(AIB1)protein in hepatocellular carcinoma(HCC).Methods Seventy-four HCC patients were obtained cancer tissue(HCC group)and adjacent normal tissues(control group)to detect the positive expression rate of AIB1 protein by immunohistochemical SP,and relative expression of AIB1 protein by Western blot.The relationship between AIB1 positive rate and pathological factors of HCC was analyzed.Results AIB1 positive products were mainly distributed in the cytoplasm of cell.The positive rate of AIB1 was significantly higher in HCC group(64.9%)than that in control group(40.5%)(P0.05),and the level of AIBI protein was higher in HCC group(105.68±4.21)than that in control group(7.57±0.29)(P0.05).AIB1 postive expression showed significant diffferences in the tumor diameter,lymph node metastasis and distant metastasis(P0.05).Conclusion The over-expression of AIB1 protein is probably associated with the development and progression of HCC.
Breast carcinoma
Clinical Significance
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S100 protein
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Objective To investigate the expression of c-myc and its relationship with the clinical feature of hepatocellular carcinoma(HCC).Methods In 59 specimens of HCC tissues and noncancerous adjacent liver tissues,the expression of c-myc was detected with immunhischemistry.Results Of the 35(59.3%)cases of HCC expression c-myc in 59 cases of HCC,the expression rate of c-myc was higher than that of the adjacent noncancerous liver and the normal liver groups(P0.05).The expression of c-myc in HCC had no association with patient′s age and sex,the tumor size and differentiated level of HCC(P0.05),but with the metastasis of HCC(P0.05).By Kaplan-Meier Log-Rank estimation,it was found that the positive expression of c-myc was associated with poorer survival compared with the negative expression(P=0.0039).Conclusion c-myc probably promotes the metastasis of HCC,and is demonstrated as the prognostic factor for patients undergoing hepatic resection.
Distant metastasis
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Objective To investigate the correlation between the clinic pathologic parameters of hepatocellular carcinoma(HCC) and the expression of beta-catenin、cyclinD1 and c-myc genes.Methods Immunohistochemical technique(EnVision~ TM plus) was applied to detect the expression of beta-catenin、cyclinD1 and c-myc proteins in 52 HCC tissues and surrounding tissues. Results The rates of positive immunostaining of beta-catenin、cyclinD1 and c-myc in the HCC tissues were 55.8% 、 48.1% and 53.8% . The rates of positive in the surrounding tissues were 36.5% , 25.0% and 32.7% . The positive rate of beta-catenin, cyclinD1 and c-myc was significantly higher in the HCC tissue than in the surrounding tissues (P 0.05 ). There was a positive correlation between the beta-catenin expression and cyclinD1 and c-myc in the HCC tissue(P= 0.0108 , r= 0.3920 ;P= 0.0295 , r= 0.3406 ). The positive rate of beta-catenin, cyclinD1 and c-myc in the HCC tissue was significantly correlated with the presence of extrahepatic metastasis,the recurrence of tumor and the differentiation of tumor(P 0.05 ). The positive rate of cyclinD1 was significantly correlated with the ortal vein tumor thrombus(P 0.05 ). The positive rate of beta-catenin was significantly correlated with the ortal vein tumor thrombus and the clinical stage (P 0.05 ). Conclusion The over-expression of beta-catenin,cyclinD1 and c-myc proteins may contribute to the proliferation of hepatocellular carcinoma, making them more aggressive and relates to the initiation and progression of HCC.
Immunostaining
BETA (programming language)
Beta-catenin
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Objective To study the expression and pathological features of total RNA and hypoxia-inducible factor-1 alpha (HIF-1α) in hepatocellular carcinoma(HCC). Methods The liver specimens from HCC patients were collected by self-control method.Total RNA was isolated and analyzed. The expression,cellular distribution,and pathological features of HIF-1α were analyzed by immohistochemistry.Results The level of liver total RNA in the tumor-adjacent tissues was obviously higher than that in the HCC ones (P0.01). The positive HIF-1α was brown and granule-like,mainly presented in cytoplasm and few in nucleus. The expression in HCC was well-distributed and weaker than that in the tumor-adjacent tissues,especially those around the central vein. The incidence was 80.0% (28/35) in HCC,and 100.0% (35/35) in the tumor-adjacent tissues,respectively (P0.001). The clinical pathological features of HIF-1α expression demonstrated that its expression was positively correlated with tumor size,and its intensity was negatively correlated with the differentiation of HCC. No correlation was found between HIF-1α and tumor numbers,HBV infection. Conclusion Abnormal nucleic acid metabolism and expression of HIF-1α were associated with the development and prognosis of HCC.
Hypoxia-Inducible Factors
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