The ENCODE (ENCyclopedia Of DNA Elements) Project
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The ENCyclopedia Of DNA Elements (ENCODE) Project aims to identify all functional elements in the human genome sequence. The pilot phase of the Project is focused on a specified 30 megabases (approximately 1%) of the human genome sequence and is organized as an international consortium of computational and laboratory-based scientists working to develop and apply high-throughput approaches for detecting all sequence elements that confer biological function. The results of this pilot phase will guide future efforts to analyze the entire human genome.Keywords:
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The Encyclopedia of DNA Elements (ENCODE) project is an international collaboration of research groups funded by the National Human Genome Research Institute (NHGRI), with the goal of delineating all functional elements encoded in the human genome. This project began in 2003 with a targeted analysis of a selected 1% of the human genome in order to testify the methods. The second phase of funding was then provided to scale the project to the entire human genome. This project has identified a large number of new DNA regulatory elements, based on novel relationships among DNA methylation, histone modification, nucleosome remodeling, and RNA-mediated targeting regulate many biological processes. The results of the second project comprised 1640 data sets, from 147 different cell types and the findings were released in a coordinated set of 30 publications across several journals. The ENCODE publications report that 80.4% of the human genome displays some functionality. The project gives us new insights into the organization and regulation of the human genome and epigenome, which significantly enhances our understanding of human health and diseases.
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ENCODE; Human genome; DNA methylation; Histone modification; Next generation sequencing
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Abstract The availability of the human genome sequence has had an enormous impact on biomedical research. New discoveries emanating directly from the elucidation of the human genome sequence have included the unexpectedly low total number of genes, the existence of numerous transcribed but noncoding sequences and the multiplicity of low‐copy repeats and segmental duplications. The Human Genome Project has also spawned new research projects such as Encyclopedia of DNA Elements (ENCODE) and Haplotype Map (HapMap) which together are helping to reveal the remarkable complexity of the human genome. Finally, comparison of the human genome sequence with the genome sequences of other higher organisms has opened up numerous research avenues in evolutionary biology.
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Abstract Identifying active cis -regulatory regions in the human genome is critical for understanding gene regulation and assessing the impact of genetic variation on phenotype. Based on rich data resources such as the Encyclopedia of DNA Elements (ENCODE) and the Functional Annotation of the Mammalian Genome (FANTOM) projects, we introduce DECRES, the first supervised deep learning approach for the identification of enhancer and promoter regions in the human genome. Due to their ability to discover patterns in large and complex data, the introduction of deep learning methods enables a significant advance in our knowledge of the genomic locations of cis -regulatory regions. Using models for well-characterized cell lines, we identify key experimental features that contribute to the predictive performance. Applying DECRES, we delineate locations of 300,000 candidate enhancers genome wide (6.8% of the genome, of which 40,000 are supported by bidirectional transcription data) and 26,000 candidate promoters (0.6% of the genome).
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The ENCyclopedia Of DNA Elements (ENCODE) Project aims to identify all functional elements in the human genome sequence. The pilot phase of the Project is focused on a specified 30 megabases (E1%) of the human genome sequence and is organized as an international consortium of computational and laboratory-based scientists working to develop and apply high-throughput approaches for detecting all sequence elements that confer biological function. The results of this pilot phase will guide future efforts to analyze the entire human genome. With the complete human genome sequence now in hand (1–3), we face the enormous challenge of interpreting it and learning how to use that information to understand the biology of human health and disease. The ENCyclopedia Of DNA Elements (ENCODE) Project is predicated on the belief that a comprehensive catalog of the structural and functional components encoded in the human genome sequence will be critical for understanding human biology well enough to address those fundamental aims of biomedical research. Such a complete catalog, or Bparts list,[ would include protein-coding genes, non–protein-coding genes, transcriptional regulatory elements, and sequences that mediate chromosome structure and dynamics; undoubtedly, additional, yet-to-bedefined types of functional sequences will also need to be included. To illustrate the magnitude of the challenge involved, it only needs to be pointed out that an inventory of the best-defined functional components in the human genome— the protein-coding sequences—is still incomplete for a number of reasons, including the fragmented nature of human genes. Even with essentially all of the human genome sequence in hand, the number of protein-coding genes can still only be estimated (currently 20,000 to 25,000) (3). Non–protein-coding genes are much less well defined. Some, such as the ribosomal RNA and tRNA genes, were identified several decades ago, but more recent
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In September,2012,more than 30 papers published on Nature,Genome Research,and Genome Biology,the ENCODE(Encyclopedia of DNA Elements) project achieved its first success.After the HGP(Human Genome Project) came to an end with a map of human genome sequence in 2001,we didn't know clearly how/where genes regulated by other factors.So,in September 2003,National Human Genome Research Institute launched a public research consortium named ENCODE,the Encyclopedia of DNA Elements,to identify all functional elements in the human genome sequence.Researchers pinpointed hundreds of thousands of landing spots for proteins that influence gene activity,many thousands of stretches of DNA that code for different types of RNA,and lots of places where chemical modifications serve to silence stretches of our chromosomes,concluding that 80% of the genome was biochemically active.Here,we review correlative information of about ENCODE and give readers instructions to recognize and apply achievements of ENCODE for human health service.
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The ENCyclopedia Of DNA Elements (ENCODE) Project aims to identify all functional elements in the human genome sequence. The pilot phase of the Project is focused on a specified 30 megabases (approximately 1%) of the human genome sequence and is organized as an international consortium of computational and laboratory-based scientists working to develop and apply high-throughput approaches for detecting all sequence elements that confer biological function. The results of this pilot phase will guide future efforts to analyze the entire human genome.
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Abstract The Human Genome Project (HGP) was initiated in 1990 and completed in 2003. It aimed to sequence the whole human genome. Although it represented an advance in understanding the human genome and its complexity, many questions remained unanswered. Other projects were launched in order to unravel the mysteries of our genome, including the ENCyclopedia of DNA Elements (ENCODE). This review aims to analyze the evolution of scientific knowledge related to both the HGP and ENCODE projects. Data were retrieved from scientific articles published in 1990–2014, a period comprising the development and the 10 years following the HGP completion. The fact that only 20,000 genes are protein and RNA‐coding is one of the most striking HGP results. A new concept about the organization of genome arose. The ENCODE project was initiated in 2003 and targeted to map the functional elements of the human genome. This project revealed that the human genome is pervasively transcribed. Therefore, it was determined that a large part of the non‐protein coding regions are functional. Finally, a more sophisticated view of chromatin structure emerged. The mechanistic functioning of the genome has been redrafted, revealing a much more complex picture. Besides, a gene‐centric conception of the organism has to be reviewed. A number of criticisms have emerged against the ENCODE project approaches, raising the question of whether non‐conserved but biochemically active regions are truly functional. Thus, HGP and ENCODE projects accomplished a great map of the human genome, but the data generated still requires further in depth analysis. © 2016 by The International Union of Biochemistry and Molecular Biology, 44:215–223, 2016.
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