A glycolytic enzyme, enolase, is recruited as a cofactor of tRNA targeting toward mitochondria in Saccharomyces cerevisiae
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Abstract:
In many organisms, mitochondria import nuclear DNA-encoded small RNAs. In yeast Saccharomyces cerevisiae , one out of two cytoplasmic isoacceptor tRNAs Lys is partially addressed into the organelle. Mitochondrial targeting of this tRNA was shown to depend on interaction with the precursor of mitochondrial lysyl–tRNA synthetase, preMsk1p. However, preMsk1p alone was unable to direct tRNA targeting, suggesting the existence of additional protein factor(s). Here, we identify the glycolytic enzyme, enolase, as such a factor. We demonstrate that recombinant enolase and preMSK1p are sufficient to direct tRNA import in vitro and that depletion of enolase inhibits tRNA import in vivo. Enzymatic and tRNA targeting functions of enolase appear to be independent. Three newly characterized properties of the enolase can be related to its novel function: (1) specific affinity to the imported tRNA, (2) the ability to facilitate formation of the complex between preMsk1p and the imported tRNA, and (3) partial targeting toward the mitochondrial outer membrane. We propose a model suggesting that the cell exploits mitochondrial targeting of the enolase in order to address the tRNA toward peri-mitochondrially synthesized preMsk1p. Our results indicate an alternative molecular chaperone function of glycolytic enzyme enolase in tRNA mitochondrial targeting.Keywords:
Enolase
Enolase
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Cells of the yeast Saccharomyces cerevisiae are transformable by DNA under non‐artificial conditions
Transformants of bakers' yeast (Saccharomyces cerevisiae) can be generated when non-growing cells metabolize sugars (without additional nutrients) in the presence of plasmid DNA. These results suggest that there is a mechanism by which DNA can naturally be taken up by the yeast cell. Natural transformation does not take place in common complete or minimal yeast culture media such as YPD and YNB. The starvation conditions used in our experiments thus seem to be an important prerequisite for such transformation events. Copyright © 2000 John Wiley & Sons, Ltd.
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The diagnostic informative value of measurements of neuron-specific enolase (NSE) in health, pulmonary cancer (including small-cell one), 8 different diseases, and pregnant patients with gestation periods from 5 to 33 weeks was assessed. Comparison of the results in each of these 10 groups with the results in health controls did not reveal any appreciable differences for any of the groups or between the tested groups. The authors consider that NSE measurements cannot be recommended for use at other than cancer institutions.
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This protocol outlines a method for extracting total lipids from Baker's yeast, Saccharomyces cerevisiae. It has been adapted from Roy et al., J. Lipid Res. 2018. doi: 10.1194/jlr.M088559.
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Neuron specific enolase (NSE) is a glycolytic enzyme, which is localized specifically to the neuronal cyloplasm and has been proposed as a biochemical marker of neuronal cell damage. The release levels of NSE into seram and cerebrospinal fluid (CSF) with traumatic brain injury (TBI) were monitored as a relevant biochemical marker for assessing the lesion degree and the prognosis of TBI. In this article, the author summarizes published primary research on NSE and TBI from the present literature, arguing that NSE is a high specific and sensitive marker for judging the outcome and the severity of brain insult, which probably contributes to the diagnosis of TBI.
Enolase
Brain damage
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One of the key feature of prions is the ability to be stable in two alternative conformations. Besides the intensively studied mammalian prions, there are also prion proteins present in the yeast Saccharomyces cerevisiae. Research in this field has lead to opposite hypotheses that explain the sense of presence of [PSI+] prion in yeast cells. Some authors postulate e of role of the prions in the evolution of S. cerevisiae, whereas other investigators point out the negative influence of these particles upon the yeast physiology. In recent years, yeast prions are used for anti-prion drug screening, because of common features with mammalian prions. This work presents the most intensively studied fields of the research carried out on [PSI+] prion in yeast.
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Fungal prion
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Ethyl methanesulfonate
Methyl methanesulfonate
Strain (injury)
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Objective To investigate the change of neuron specific enolase(NSE) in epileptic children after seizure.Methods The levels of serum NSE in 27 patients within 72 hours after seizure were measured by enzyme-linked immunosandwich assay.Results The levels of serum NSE in the epileptic patients were much higher than that of the control group(P0.01).Conclusion NSE in serum could reach higher levels after seizure ,suggesting that neuronal damage may result from seizure.
Enolase
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Neuronal damage
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