On-Line Screening and Identification of Radical Scavenging Compounds Extracted from Flos Lonicerae by LC-DAD–TOF-MS
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Chlorogenic Acid
Objective To explore effect of different processing methods on content of rutin in Flos sophorae.Methods HPLC method was employed to determine the content of rutin in Flos sophorae.Results Processing temperature and time increased,content of rutin decreased gradually.Conclusion The change of processing temperature and time had great effect on content of rutin in Flos sophorae.
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Objective:In order to raise extract ion ratio and reduce solvent waste, the extraction technology of Flos Lonicerae was improved. Methods:First, the Flos Lonicerae was humidified with litt le ethanol-water solution, and then the chlorogenic acid was extracted by boilli ng water with whisk. Results:The extraction ratio of chlorogenic acid was 7.8 9 %. The content of chlorogenic acid was 16.7% in extract. These results were h igher than those of traditional extraction methods, but the wastage of ethanol o nly was 6% of extracting with ethanol. Conclusion:The new technology has available value in extra ction of chlorogenic acid.
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Objective: To optimize the extraction process of chlorogenic acid in flos lonicerae.Methods: The flos lonicerae was treated by means of cellulase and/or pectolase before extracted by ethanol reflux, studying the enzyme dosage, treatment time, treatment temperature how to influence the percentage of extraction of flos lonicerae and the amount of chlorogenic acid in extrction.Results: The percentage of extraction of flos lonicerae and the amount of chlorogenic acid in extrction could be obviously increased by the cellulase treatment, 8.15g chlorogenic acid could be extracted from 100g flos lonicerae. The optimum temperature of treatment was 40℃～50℃; The percentage of extraction was higher by the combined treatment of cellulase and pectilase than by cellulase only, but the amount of chlogenic acid was not obviously different between these two methods.Conclusion: The amount of chlorogenic acid in extrction could be increased by enzymic treatment.
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The content of chlorogenic acid and rutin in corn silk was simultaneously determined by RP-HPLC to explore its measuring method.The results showed that the linear range was 0.144~ 0.864 μg(r = 0.999 4) for chlorogenic acid and 0.281~1.686 μg(r = 0.999 2)for rutin,the average recoveries(n = 5) of chlorogenic acid and rutin were 100.8% and 98.6 %,respectively.The method is rapid,accurate with good reproducibility,which can be applied in simultaneous determination of chlorogenic acid and rutin in corn silk.
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To select the best processing method for shanlucha and establish a method for content determination of chlorogenic acid and rutin in shanlucha.Using HPLC to determine the contents of chlorogenic acid and rutin in shanlucha.Chlorogenic acid and rutin can be determined by HPLC,the chlorogenic acid showed a good linear relationship in a range of 0.01032～0.6192μg,r=0.9998,the average recovery was 98.86% and RSD was 2.54%,and the rutin also showed a good linear relationship in a range of 0.02004～1.2024,r=0.9999,the average recovery was 102.31% and RSD was 2.17%.The method is simple,accurate,with strong specificity,which can be used for the quantitative determination and control of shanlucha.
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To investigate the stability of chlorogenic acid in extract of flos lonicerae in different conditions.The stability of chlorogenic acid in extract of flos lonicerae in phosptat buffe with different pH values, methanol, ethanol and different base solutions(Ca(OH)2 and NaOH) was investigated by the classical isothermal method.The experiments showed the chlorogenic acid in extract of flos lonicerae was more stable in acidic water than in basic water. It was stable in these organic solutions and base solution[Ca(OH)2].In different conditions, the stability of chlorogenic acid in extract of flos lonicerae was different. It provided a reference to the extraction and analysis of chlorogenic acid and production of chlorogenic acid preparation.
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[Objective] To compare the content of chlorogenic acid and rutin in the different parts of Galium verum L.by using the HPLC-DAD method.[Method] A HPLC-DAD method was developed for the content determination of rutin and chlorogenic acid in root,stem,leafage and flower from Galium verum L..[Result] A good linearity was obtained,the linear range were: rutin 0.024 3-1.950 0 μg,chlorogenic acid 0.132 5-10.600 0 μg;the average recovery of rutin was 101.02% and the average recovery of chlorogenic acid was 101.65%.The content of rutin and chlorogenic acid was the highest in flowers and the lowest in roots.[Conclusion] The HPLC-DAD method separation is good,stable and can be used for the quality control of Galium verum L.,the contents of rutin and chlorogenic acid are different in the different parts of Galium verum L..
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[Objective] The aim of the study was to determine chlorogenic acid,rutin and luteolin contents in Flos Lonicerae simultaneously.[Method] With Zorbax SB-C18 column(250 mm×4.6 mm,5 μm),methanol-0.02% Phosphoric acid(50∶50)as mobile phase,flow velocity at 1.0 ml/min,detection wave length at 350 nm and column temperature at 30 ℃,the chlorogenic acid and the flavonoid compounds contents in Flos Lonicerae were determined by RP-HPLC.[Result] The chlorogenic acid,rutin and luteolin contents have obvious differences in different kinds of Flos Lonicerae.[Conclusion] The method is simple,feasible,and can be used to control the quality of Flos Lonicerae.
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Objective:To develop an R P - HPLC method for determination of chlorogenic acid and rutin simuhane- ously in Flos Farfarae.Methods:Chromatorax C_(18) column(4.6 mm×250 mm,5μm) was used,the mobile phase was acetonitrile-1% acetic acid(20:80) at a flow rate of 1.0 mL·min~(-1).The UV detection wavelength was 254 nm,and the column temperature was 30℃.Results:The linear range was 0.0449-0.4490 mg·mL~(-1) (r= 0.9999,n=6) for chlorogenic acid and 0.0264-0.2640 mg·mL~(-1) (r=0.9999,n=6) for rutin.The average re- coveries(n=5) of chlorogenic acid and rutin were 99.9% and 101.2%,respectively.Conclusion:The method is found to be simple and accurate for simultaneous analysis of chlorogenic acid and rutin in Flos Farfarae.The method may be applied for quality control.
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