[Studies on stability of chlorogenic acid in extract of Flos lonicerae].
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Abstract:
To investigate the stability of chlorogenic acid in extract of flos lonicerae in different conditions.The stability of chlorogenic acid in extract of flos lonicerae in phosptat buffe with different pH values, methanol, ethanol and different base solutions(Ca(OH)2 and NaOH) was investigated by the classical isothermal method.The experiments showed the chlorogenic acid in extract of flos lonicerae was more stable in acidic water than in basic water. It was stable in these organic solutions and base solution[Ca(OH)2].In different conditions, the stability of chlorogenic acid in extract of flos lonicerae was different. It provided a reference to the extraction and analysis of chlorogenic acid and production of chlorogenic acid preparation.Keywords:
Chlorogenic Acid
Flos
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The high performance liquid chromatographic method was developed for the determination of chlorogenic acid in Flos Lonicerae.The chromatographic separation was carried out in a Nucleosil C18 column(4.6×250 mm,5 μm) with a mobile phase consisting of methanol-0.4 %H3PO4(25∶75,V/V) mixture.The detection wavelength of photodiode array detector was set at 327 nm and the column temperature was set at 25 ℃.The calibration curve was linear in the range of 0.64～6.40 μg for chlorogenic acid(r=0.999 6).The average recovery of chlorogenic acid was 99.3 % with a relative standard deviation(RSD)of 0.8 %.The method was simple,reliable and suitable for quality evaluation and control of Flos Lonicerae.
Flos
Chlorogenic Acid
Relative standard deviation
Chromatographic Separation
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A fast method based on dynamic microwave-assisted extraction(DMAE) coupled on-line with high-performance liquid chromatography(HPLC) by means of a flow injection interface has been developed for the determination of chlorogenic acid in Flos Lonicerae japonicae. The extraction was performed in a recirculating system.When a number of extraction cycles were completed,the fractional extract(20 μL) was driven to the analytical column by mobile phase and was measured by a UV detector.The extraction conditions were examined and optimized.The detection and quantification limits of chlorogenic acid obtained at optimized conditions are 0.25 and 0.83 μg/mL,respectively.The intra-day and inter-day precisions(RSD) obtained are 3.4% and 4.1%,respectively.The mean recovery of chlorogenic acid is 98.1%.Compared with ultrasonic extraction used in the Chinese pharmacopoeia,the proposed method reduces the amount of solvent, provides more rapid measurement and high extraction yield.
Flos
Chlorogenic Acid
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Objective:The best operation condition of extraction was found through analyzing the influences of both process of microwave pretreatment and hot wave washing. Methods: A new extraction method of chlorogenic acid was that the dry FLos Lonicerae was humidified with 75% ethanol-water solution at first, then the wet Flos Lonicerae was rapidly vaporized by microwave heating, at last chlorogenic acid was extracted after hot water washed twice, 10 minutes each time. Results: Compared with traditional extraction, the extraction time was about less than 6 times and then extraction ratio was increased by 1%. Conclusion: The method has high rate of extraction and short time of extraction.
Flos
Chlorogenic Acid
Water extraction
Hot water extraction
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To make a rapid and accurate method for determination of chlorogenic acid in the extraction of Flos Lonicerae by reversed-phase HPLC.Experiments were carried out on a Kromasil C18 collumn(250mm×(4.6)mm,5μm);mobile phase:a solution of 0.3% phosphoric acid-acetonitrile(90∶10,V/V);detection wavelength:327nm;injection volume:20μL.Chlorogenic acid in the extraction of Flos Lonicerae was well separated.Chlorogenic acid had a good linearity in the range of 0.0256～0.0896 mg·mL and the average recovery rate was 97.58%.The new method was suitable for determination of chlorogenic acid in the extraction of Flos Lonicerae by reversed-phase HPLC.
Chlorogenic Acid
Flos
Phosphoric acid
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In this paper,three sample preparation for determination of chlorogenic acid in flos lonicerae was studied.Test results showed that the time spent on high-speed homogenization extraction was obviously less than the ultrasonic extraction and the hot reflux extraction.The chlorogenic acid was extracted from samples by high-speed homogenization with 80% methanol solution.The extract was separated on a Waters Xterra RP18 column(3.9mm×150 mm,5 μm) using 1% acetic acid as the mobile phase with gradient elution,the flow rate was 0.8 mL/min.The detection wavelength was 345 nm.Under this condition,the linear of chlorogenic acid was in the range of 5.0—200 μg/mL.The average recoveries of the samples were 118%,and the relative standard deviation was 4.5%(n=6).This method for the extraction and determination of chlorogenic acid in flos lonicerae has good results.
Flos
Chlorogenic Acid
Homogenization
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Chlorogenic acid was extracted from flos lonicerae by microwave-assisted extraction(MAE).Ultraviolet(UV) ray was used for the quantitative and qualitative determination of chlorogenic acid from flos lonicerae.The effects of solvent types and concentration,MAE time,liquid/solid(ratio,) pH of solvent and extraction degrees on extraction of chlorogenie acid were discussed.Orthogonal experiment was(employed) to investigate the optimum technological condition.The optimum extraction conditions were as follows: ratio of enthanol solution(φ=0.4) to solid,15;MAE time,1 min;extraction twice.Compared with other techniques,MAE is fast,simple,high efficient,energy-saving and environmental friendly.
Flos
Chlorogenic Acid
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[Objective] To establish a HPLC method for quantitative determination of luteolin-7-O-glu-coside and chlorogenic acid in Flos Lonicerae Japonicae using gradient elution method.[Methods] Determining column: Xterra@ RP 185;Detector: Waters 2 998 diode array detector(DAD);Workstation: Waters Empower;The mobile phase: 0.5% acetic acid-acetonitrile;Linear gradient elution: 0~15min(90 ∶ 10-80 ∶ 20),15~30 min(80 ∶ 20-75 ∶ 25),30~40 min(75 ∶ 25-65 ∶ 35),40~45 min(65 ∶ 35-90∶ 10);The flow rate was 1 mL/min,and the detection wavelength was set at 350 nm.[Results] In this chromatographic conditions,the two components were completely separated.[Conclusion] The method is simple,accurate,fast and easy,which can detect two components of Flos Lonicerae Japonicae simultaneously.
Flos
Chlorogenic Acid
Gradient elution
Chromatography detector
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We summed up the common methods of the extraction process of chlorogenic acid in Flos Lonicerare to determine the optimal method of extracting chlorogenic acid with the results:The extraction process of acid ethanol is optimum.The optimum extraction conditions are 80% ethanol,pH=5,refluxing 0.5h.The content of chlorogenic acid is fairly high.It is close to the optimum extraction condition,that are 75% ethanol,pH=4,refluxing 2.0h,and it adapt to industry production.
Flos
Chlorogenic Acid
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The optimum conditions for the extraction of chlorogenic acid from Flos Lonicerae with ethanol water as the solvent were studied.The effects of 4 factors(extraction temperature,extraction time,the ratio of material to solvent,ethanol concentration) on the extraction rate of chlorogenic acid were investigated.Through orthogonal experiments,the optimum conditions were determined as:extraction temperature 65℃,extraction time 20 min,the ratio of material to solvent 1∶15,and ethanol concentration 80%.Under such conditions,the extraction rate of chlorogenic acid reached 7.953%.It can be used as a valuable reference for the industrial production of chlorogenic acid.
Chlorogenic Acid
Flos
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We summed up the common methods of the extraction process of chlorogenic acid in Flos Lonicerare to determine the optimal method of extracting chlorogenic acid with the results:The extraction process of ethanol B is optimum.The optimum extraction conditions are 6 fold of 40% ethanol,pH=6,dip in 80℃ water,refluxing 2.5h.The content of chlorogenic acid is fairly high.
Flos
Chlorogenic Acid
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