MicroRNA-141 inhibits migration of gastric cancer by targeting zinc finger E-box-binding homeobox 2
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Human microRNA (miR)-141 is a member of the miR‑200 family, which has been reported to be downregulated in gastric cancer, and involved in the proliferation of gastric cancer cells. However, little is currently known regarding its role in the migration of gastric cancer. The present study investigated the function of miR‑141 in gastric cancer cell migration, and evaluated the contribution of zinc finger E‑box‑binding homeobox 1 and 2 (ZEB1/2) in miR‑141 mediated migration of gastric cancer cells. The expression levels of miR‑141 and its potential ZEB1/2 targets were examined by quantitative polymerase chain reaction (qPCR) and western blotting, respectively. The migration of SGC‑7901 and HGC‑27 gastric cancer cells, which had been transfected with an miRNA precursor, was examined by cell migration and wound healing assays. A luciferase activity assay was used to validate whether ZEB1/2 was a direct target of miR‑141. The results demonstrated that overexpression of miR‑141 markedly inhibited the migration of gastric cancer cells in vitro. Forced overexpression of miR‑141 significantly reduced the luciferase activity of the 3'‑untranslated region of ZEB2 in gastric cancer cells. Furthermore, the mRNA and protein expression levels of ZEB2 were reduced in cells overexpressing miR‑141, whereas the protein expression levels of E‑cadherin were increased. In gastric tumor samples the expression levels of ZEB2 were inversely correlated with the expression of miR‑141. These results suggest that miR‑141 may be involved in the inhibition of gastric cancer cell migration, and that ZEB2 is a target gene of miR-141.锌 finger-homeodomain 蛋白质(ZHD ) 在许多植物是在场的；然而， ZHD 基因家庭的进化历史仍然保持大部分未知。我们这里证明 ZHD 基因是植物特定的，将近所有 intronless，并且与微型锌手指(MIF ) 有关，拥有仅仅锌的基因摸。从代表性的陆生植物的 ZHD 基因的种系发生的分析建议非种子植物 ZHD 基因占据基础位置，被子植物相当或相同的事物形成七不同 clades。几 clades 从二包含基因或更多主修被子植物组，包括的 eudicots，单音的简易窄床， magnoliids，和其它基础被子植物，显示几复制发生在 flowering 的多样化前。另外，特定的系经历了更最近的复制。不同于 ZHD 基因， MIF 仅仅从种子植物被发现，可能在种子植物的分叉前由 homeodomain 的损失源于 ZHD。而且， MIF 基因也经历了相对最近的基因复制。最后，染色体复制可能在被子植物实质地作出贡献到家庭尺寸的扩大并且在这些基因引起了功能的冗余性 / 重叠的高水平。
EMX2
Homeobox A1
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Zinc finger E-box-binding homeobox 1 (1124 aa, ~124 kDa) is encoded by the human ZEB1 gene. This protein is involved in both the transcriptional repression of interleukin 2 and the positive regulation of neuronal differentiation.
Homeobox A1
HNF1B
LIM domain
Homeobox protein Nkx-2.5
EMX2
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There was a highly conservative homeobox in nearly all homeobox gene family,which used for encoding transcription regulatory factors(homeobox protein) and generated the accurate regulating action in cells proliferation and differentiation.The mutation of homeobox gene not only resulted in development malformation,but also had a close relationship with the occurrence and development of some tumours.The structural features of homeobox gene and the effect in tumours were summarized.
Homeobox A1
Homeobox protein Nkx-2.5
HNF1B
EMX2
DLX5
CDX2
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Homeobox A1
Homeobox protein Nkx-2.5
HNF1B
CDX2
EMX2
DLX5
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Zinc finger nuclease
LIM domain
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Abstract Over the past 25 years, numerous homeobox genes have been identified and their functions have been elucidated. Many studies in various plant species have revealed that Knotted‐like homeobox ( KNOX ) genes, the first identified homeobox gene in plants, play a profound role in shoot apical meristem organisation, maintenance, and leaf initiation. In addition to the KNOX gene, numerous other homeobox genes have been discovered (e.g., BEL1‐like homeodomain , Homeodomain leucine zipper , and WUSCHEL‐related homeobox genes) and play important roles in plant development. Moreover, in recent years, it has become clear that the homeobox genes also contribute to morphological diversity in plants. Key Concepts Homeobox (HB) genes encode a subset of transcription factors that contain a homeodomain In plants, several groups of HB genes (e.g., KNOTTED‐like homeobox , BEL1‐like homeodomain , Homeodomain leucine zipper , and WUSCHEL‐related homeobox genes) have been shown to have a distinct role in the development. The HB genes are involved in a wide range of developmental processes (e.g., stem cell maintenance in the SAM and floral meristems, cambium, and root meristem, leaf development, vascular development, embryo patterning, etc.). Recent studies revealed that changes in HB genes led to leaf form diversification and that HB genes are involved in alteration in response to environmental changes in a single plant.
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EMX2
Homeobox protein Nkx-2.5
HNF1B
DLX5
CDX2
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Homeobox protein Nkx-2.5
Homeobox A1
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Homeobox A1
Homeobox protein Nkx-2.5
LIM domain
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The goldfish (Carassius auratus) visual pathway displays continuous growth and plasticity throughout life. Since homeobox genes are important transcriptional regulators in development, we searched for homeobox genes in the adult goldfish retina and brain. Using the PCR, we discovered a repertoire of homeobox sequences expressed in these tissues. In addition to isolating homeodomain sequences found in the vertebrate Hox gene clusters, a sequence identical to the chicken CHox7 homeodomain was characterized. Furthermore, a sequence with significant homologies to the Xenopus XIHbox8 and leech Htr-A2 homeodomains was identified, and these sequences may define an additional class of homeodomain. Finally, a sequence belonging to the paired class (prd) of homeodomains is reported. Homeobox gene expression in the adult goldfish retina and brain may be associated with the persistent developmental features of these tissues.
Homeobox A1
EMX2
Homeobox protein Nkx-2.5
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Lernaean Hydra
Homeobox protein Nkx-2.5
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