Transgenic Nicotiana tabacum plants expressing a fungal copper transporter gene show enhanced acquisition of copper
12
Citation
34
Reference
10
Related Paper
Citation Trend
Keywords:
Plant biochemistry
The commercial application of genetically modified plants has been seriously impeded by public concern surrounding the potential risks posed by such plants to the ecosystem and human health. Previously, we have developed a ‘pollen- and seed-specific Gene Deletor’ system that automatically excised all transgenes from the pollen and seeds of greenhouse-grown transgenic Nicotiana tabacum. In this study, we conducted seven field experiments over three consecutive years to evaluate the stability of transgene excision under field conditions. Our results showed that transgenes were stably excised from transgenic Nicotiana tabacum under field conditions with 100% efficiency. The stability of transgene excision was confirmed based on PCR, as well as the GUS staining patterns of various organs (roots, leaves, petiole, stem, flower, fruit, and seeds) from transgenic N. tabacum. In six transgenic lines (D4, D10, D31, D56, and D43), the transgenes were stably deleted in the T0 and T1 generations. Thus, the ‘Gene Deletor’ system is an efficient and reliable method to reduce pollen- and seed-mediated unintentional gene flow. This system might help to alleviate the food safety concerns associated with transgenic crops.
Cite
Citations (0)
Southern blot
Northern blot
Dot blot
Cite
Citations (35)
Southern blot
Northern blot
Cite
Citations (2)
Cauliflower mosaic virus
Cite
Citations (73)
Abstract Asymmetric epoxidation of digeranyl, which is a squalene analog, with cultured cells of Nicotiana tabacum was investigated. Feeding of [8‐ 3 H]‐digeranyl into the cultured cells of N. tabacum resulted in the formation of (3 S )‐2,3‐epoxydigeranyl and 6,7‐epoxydigeranyl. It was found that the epoxidation of digeranyl with N. tabacum was highly stereoselective. Copyright © 2003 John Wiley & Sons, Ltd.
Squalene
Cite
Citations (1)
We have synthesized optically active α-phenylpyridylmethanols by reduction or hydrolysis with cell cultures of Nicotiana tabacum or immobilized cells of N. tabacum.
Plant cell
Cite
Citations (22)
Cite
Citations (42)
Ribonuclease III
Cite
Citations (6)
Nicotiana tabacum (Solanaceae) is the only species whose leaves can be legally marketed as tobacco according to the Japanese Tobacco Business Act. Nicotine, a major alkaloid produced by N. tabacum leaves, is regulated in pharmaceuticals by the Japanese Pharmaceutical Affairs Law. However, the use of N. tabacum stems as an excipient in pharmaceuticals is permitted, because these contained only a small amount of nicotine. Recently, several reports showed that a substantial amount of nicotine was detected in an OTC pharmaceutical product, in which N. tabacum stems were used as an excipient. Therefore, products containing N. tabacum stems could be contaminated with the leaf material. In the present study, we established a method to detect contamination of N. tabacum stem materials with its leaves, using microscopy to obtain standard reference microphotographs for identification. Cultivated N. tabacum stems and leaves, commercial cigarette leaves, and N. tabacum tissue imported as excipient material were used for preparing the microphotographs. The characteristic N. tabacum leaf structures found in the powdered fragments included: epidermal cells with sinuous anticlinal cell walls, hairs, mesophyll parenchyma with crystalized calcium oxalate (calciphytoliths), and branching vascular bundles derived from reticulate net-veins. A comparison of the microscopic characteristics of an OTC powder with those from the standard reference microphotographs was an effective method for N. tabacum stem and leaf identification. Thus, we evaluated the powdered pharmaceutical product containing N. tabacum stem tissue and Hydrangea serrata (Hydrangeaceae) leaf tissue as excipients, and confirmed the presence of N. tabacum leaf material.
Excipient
Cite
Citations (2)