Improvement of Shake Flask Fermentation Conditions for the Pseudomonas Strain N1
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To improve the yield of the Pseudomonas strain N1 in the fermentation culture medium screened from the biofilm of BAF reactor, the optimal shake flask fermentation conditions for Pseudomonas N1 were determined by single factor test and orthogonal test. The composition of optimal fermentation culture medium was as follows: nitrate of potash of 4 g/L, sodium succinate of 26.1 g/L, KH 2 PO 4 of 4.0 g/L, MgSO 4 ·7H 2 O of 1 g/L, KCl of 1 g/L. The cell concen- tration could reach 9.08 g/L under the conditions. Fermentation was performed for 36h in a 250ml shake flask containing 70mL medium at 1% inoculums volume and initial pH value 7.0. The cell concentration could reach 12.86 g/L which was 2.5 fold higher than that under the original conditions. Simultaneous nitrification and denitrification could be achieved to alleviate the pollution of nitrogen in the wastewater by adding the microbial inoculums into the common aerobic nitrifica- tion sludge.Keywords:
Strain (injury)
Potassium nitrate
The effects of micro-nanometer aeration on the nitrogen removal of a Thalia dealbata floating-bed was investigated by comparing the nitrogen removal of a group with three treatments of micro-nanometer aeration,a group with air blow aeration and a group without aeration(the control group).The results showed that compared with the group without aeration,the removal rates of TN and NH4+-N were increased by 13.35% and 21.72%,respectively,in the micro-nanometer aeration plant floating-bed,and increased by 5.64% and 10.61%,respectively,with the air blow aeration method.However,the high concentrations of DO formed by the micro-nanometer aeration had a negative effect on the removal of NO3——N.The removal rates of NO3——N in this group were lower than those of the air blow aeration group and the control group.By comparing the results from the micro-nanometer aeration group with those from the control group,micro-nanometer aeration was found to significantly improve the growth of plants.By the end of the experiment,the biomass in this group had increased by 65.38%,while the biomass increase in the air blow aeration group and the control were 21.05% and 63.93%,respectively.The total nitrogen uptake by the plants was also different among the treatments.The highest uptake of TN was obtained in the micro-nanometer aeration group(90.60 mg),compared to 54.84 and 63.42 mg in the air blow aeration and the control group,respectively.In conclusion,micro-nanometer aeration was the most effective way of increasing DO,changing the microenvironment of the rhizosphere,and enhancing the growth of ammonifying and nitrifying bacteria,but it retarded the growth of the denitrifying bacteria.
Nitrogen gas
Nanometre
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The genome analysis of the plant growth-promoting rhizospheric Pseudomonas sp. strain OA3, isolated from maize in North West Province, South Africa, is reported in this study. Pseudomonas sp. strain OA3 exhibits plant growth-promoting ability by enhancing maize and soybean growth.
Strain (injury)
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A bacterial strain, Pseudomonas bohemica strain ins3 was newly isolated as a resistant strain against high concentrations of hinokitiol. This strain was revealed not only to show resistance but also completely remove this compound from its culture broth. In addition, its mechanism was revealed to be independent of conventional aromatic dioxygenases, ie catechol-1,2- or 2,3-dioxygenases.
Strain (injury)
Catechol
Bacterial strain
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Many Staphylococcus and Pseudomonas species, such as Staphylococcus aureus and Pseudomonas aeruginosa , are opportunistic human pathogens. However, Pseudomonas species are also known to produce bioactive compounds. Here, we report on the genome sequences of a Pseudomonas isolate and a Staphylococcus species of unknown origin that it inhibits.
Strain (injury)
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A pulse aeration bio-contact oxidation process was used to treat urban riverway water in lab-scale test.The optimal process parameters are as follows;the aeration intensity is4m~3/(m~2 ? h), the HRT is 2 h,the ratio of non-aeration to aeration is 6:4 to 8:2,and the aeration period is equal to or less than 2 min.The average removal rates of NH_4~+- N and COD——(Mn) are 78.30%to 93.51%and 21.12%to 24.29%respectively.The biomass on bio-media is 265.53 to 938.63 nmol/m~3.The effect of pulse aeration bio-contact oxidation process is better than that of continuous aeration bio-contact oxidation process.The pulse aeration bio-contact oxidation process saves 60%to 80%air volume compared with continuous aeration bio-contact oxidation process.A pulse water-lifting aeration in-situ water quality purification device was developed by combining water-lifting aeration with biological contact oxidation. The pilot study results show that the average removal rates of NH——4~+- N,TN and COD——(Mn) are 55.13%, 18%and 10%respectively when the water temperature is less than 15℃.
Oxidation process
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This study examined the effects of oxygen supplementation on fermentation performance. Two types of fermentations were performed in 6-gal. pilot-scale fermentors: 1) wort was aerated to saturation and pitched; 2) wort was aerated to saturation, pitched, and given additional air for 6 hr. The fermentations were monitored through primary and secondary stages. Several parameters were examined: yeast count, extract, alcohol concentration, degree of fermentation, and pH. The fermentations with extended aeration were somewhat more vigorous during primary fermentation and had a lower pH. By the end of secondary fermentation, the differences observed were reduced; however, the pH remained substantially lower with extended aeration. Pyruvic acid in the finished beer was considerably higher under extended aeration, whereas sulfur dioxide and acetaldehyde were lower. Flavor evaluation showed preference for the extended aeration treatment.
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牛乳様の外観を持つように製造された褐色化の著るしくないミルク・キャラメルの貯蔵中のアミノ・カルボニル反応性諸変化に及ぼすaerationの影響を,26°, 70%相対湿度の貯蔵条件で検討し,以下のことを明らかにした. (1) Aerationは脂質性ステールフレーバーの発生を促進する. (2) Aerationによって褐色化が促進され,又,赤血塩還元値もより増加する. (3) したがって,これらのaerationに起因する諸欠陥を阻害する為には, aerationの工程をその効率限度内で最少限に行う必要がある.
Browning
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Strain (injury)
Polyvinyl Alcohol
Xanthomonas
Pseudomonadales
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An intermittent aeration biological contact oxidation process was used to treat micro-polluted water in experiment.The removal effect of NH4+-N and CODMn by pulse aeration biological contact oxidation was studied under different intermittent conditions,and superiority of this technology comparing to continuous aeration bio-contact oxidation process was discussed.The results showed that when the intensity of aeration is 4 m3/(m2·h) and the ratio of without aeration time to aeration time is 6:4 to 9:1,the average removal rate of NH4+-N and CODMn achieved 69.49% to 93.51% and 21.12% to 24.29%,respectively,and the biomass of bio-media was 265.53 nmol/m3 to 938.63 nmol/m3.The effect of intermittent aeration biological contact oxidation process to treat micro-polluted water is better than that in continuous aeration biological contact oxidation process when the ratio of without aeration time to aeration time is not less than 8:2 and the dissolved oxygen is not less than 4 mg/L.The intermittent aeration biological contact oxidation process saved 60% to 80% air volume than that of continuous aeration.
Oxidation process
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This study examined the effects of oxygen supplementation on fermentation performance. Two types of fermentations were performed in 6-gal. pilot-scale fermentors: 1) wort was aerated to saturation and pitched; 2) wort was aerated to saturation, pitched, and given additional air for 6 hr. The fermentations were monitored through primary and secondary stages. Several parameters were examined: yeast count, extract, alcohol concentration, degree of fermentation, and pH. The fermentations with extended aeration were somewhat more vigorous during primary fermentation and had a lower pH. By the end of secondary fermentation, the differences observed were reduced; however, the pH remained substantially lower with extended aeration. Pyruvic acid in the finished beer was considerably higher under extended aeration, whereas sulfur dioxide and acetaldehyde were lower. Flavor evaluation showed preference for the extended aeration treatment.
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