STUDIES ON THE DEPOSITION OF LAMININ AND TYPE IV COLLAGEN IN HUMAN ORAL MUCOSA TRANSPLANTED TO NUDE MICE
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Normal human oral mucosa (20 biopsies) and similar tissue transplanted to nude mice (44 transplants) were investigated for the presence of laminin and type IV collagen, using an indirect immunofluorescence staining technique. Laminin and type IV collagen were consistently found in the basement membrane area of the epithelial-stromal junction, and of the vessels of both normal oral mucosa and oral mucosal transplants. De novo synthesis of laminin and type IV collagen by transplanted epithelium was evidenced by the finding of an increased thickness of basement membrane deposits. New basement membrane-like material was also seen between the migrating human epithelial cells and the murine connective tissue. By application of species-specific monoclonal antibodies to human laminin and type IV collagen, it was demonstrated that human epithelial cells have the ability to participate in the information of their basement membranes. The use of both species-specific and species-unspecific antibodies showed that the vessel supply of the transplants was of both human and murine origin.Keywords:
Type IV collagen
Oral mucosa
Immunofluorescence
Normal human oral mucosa (20 biopsies) and similar tissue transplanted to nude mice (44 transplants) were investigated for the presence of laminin and type IV collagen, using an indirect immunofluorescence staining technique. Laminin and type IV collagen were consistently found in the basement membrane area of the epithelial-stromal junction, and of the vessels of both normal oral mucosa and oral mucosal transplants. De novo synthesis of laminin and type IV collagen by transplanted epithelium was evidenced by the finding of an increased thickness of basement membrane deposits. New basement membrane-like material was also seen between the migrating human epithelial cells and the murine connective tissue. By application of species-specific monoclonal antibodies to human laminin and type IV collagen, it was demonstrated that human epithelial cells have the ability to participate in the information of their basement membranes. The use of both species-specific and species-unspecific antibodies showed that the vessel supply of the transplants was of both human and murine origin.
Type IV collagen
Oral mucosa
Immunofluorescence
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Abstract Forty laryngeal carcinomas were studied by immunofluorescence with specific antisera against components of the basement membrane (type IV collagen and laminin) as well as antisera against connective tissue antigens (type V collagen and fibronectin). The basement membrane surrounding well‐differentiated squamous cell carcinomas showed an appearance similar to that seen beneath normal epithelium. In contrast, marked alterations of the basement membrane were constantly observed around infiltrating and poorly‐differentiated carcinomas. The staining of connective tissue components in most cases was as intense in carcinomas as in normal laryngeal mucosa. The use of antibodies to basement membrane components may help to elucidate the mechanism of invasion of connective tissues by malignant cells.
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Immunofluorescence
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Type IV collagen
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We used immunofluorescence and immunohistochemical PAP methods on 22 paraffin-embedded liver tissue specimens for observation and analysis of the distribution of extracellular matrix (ECM) elements in chronic hepatitis and cirrhosis. Our study revealed that in CLH there was only mild increases of types III, V collagen and fibronectin in spotty necrosis areas. In CPH, types III, V collagen and fibronectin revealed mild to moderate increase in portal area and lobular sinusoid. In CAH, moderate to marked increases of types III, V collagen and fibronectin and mild increase of type IV collagen in portal area, sinusoid lining, piecemeal necrosis and fibroseptum were found. Types I, IV collagen in fibroseptum were also noted. Some periportal hepatocytes showed abundant intracellular fibronectin. In cirrhosis, cases expressed similar finding to CAH except much more type IV collagen deposition. In addition, the basement membrane components including type IV collagen and laminin were found in the "capillarization" of periportal sinusoids in fibrotic liver tissue. In areas of piecemeal necrosis, the hepatocytes, single or assembled in "rosettes", were underlined by linear deposits of laminin and type IV collagen. Our study revealed that, histologically, the ECMs distribution of CAH is similar to that of cirrhosis but could be clearly distinguished from CPH and CLH. The prominent changes of ECMs, especially the basement membrane components, in case of CAH and cirrhosis are consistent with the fact that ECM may play a central role in liver function impairment and fibrogenesis.
Type IV collagen
Sinusoid
Lobules of liver
Type I collagen
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Objective To find out the distribution of extracellular matrix (ECM) in squamous cell carcinoma (SSC) and explore its biological significance. Methods Fifty cases of SSC were studied by immunohistochemistry with antibodies to laminin (LN), fibronectin (FN), PCNA, and p53. Results Three types of distribution patterns were found. i.e., discontinuous peri carcinoma nest type, fragmentary type, and vascular stroma type. The three types were correlated closely with the growth, differentiation and proliferation of tumor. Basement membrane was present in 3 cases with large invasive tumor nests, shown by continuous linear distribution of FN and LN. Conclusion The expressions of FN and LN decrease markedly, but the absence of basement membrane does not necessarily define invasive SSC, and the correlation of the presence of basement membrane and histologic pattern of invasive tumor suggests that SSC is capable of undergoing focal differentiation before invasion and metastasis have occurred. The distribution of FN and LN might represent the malignant degree of SSC.
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Matrix (chemical analysis)
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Abstract: Chronic clinical irritation of the palatal mucosa by dentures involves a series of histological changes in epithelial and connective tissues, inflammatory cells and the vasculature. No single change is pathognomic of this inflammatory process. The rupture of basement membrane associated with the development of denture stomatitis often marks an important stage. This study investigated modifications in basement membrane organisation, especially the distribution of type IV collagen and a specific laminin chain (α1), during denture stomatitis. Biopsies of palatal mucosa were obtained from 12 patients (8 with denture stomatitis and 4 with clinically healthy mucosa) who had worn removable dentures for more than 3 years. Immunohistochemical studies performed with specific antibodies to type IV collagen and a laminin (α1) revealed strong expression in the basement membrane of healthy palatal mucosa. In denture stomatitis, some discontinuities or disruptions in basement membrane were observed at the interface between connective tissue and epithelial cells. These findings suggest a relationship between the expression of laminin (α1) and type IV collagen and the development of denture stomatitis, a disorder involving modification of soft tissues in which initial inflammation of the palatal mucosa results from stress under the denture. These changes in basement membrane can be detected by histological studies.
Type IV collagen
Oral mucosa
Mucous membrane
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As there are few recent reports concerning the structure and exact composition of the extracellular matrix from human normal and varicose veins, we carried out comparative immunohistochemical analysis of vessel wall using conventional and confocal laser scanning immunofluorescence techniques. The present report is a rapid review of the structure and function of the 19 known collagen types and our first results on the distribution of collagen types VI, XII and XIV and laminin (glycoprotein from basement membrane) in vein walls. Type VI collagen is concentrated in the sub-endothelium and widely distributed in the media and adventice. For the first time, we demonstrated that both FACIT (fibril-associated) collagens XII and XIV were present in the vein wall, but at different anatomic sites.
Immunofluorescence
Type IV collagen
Matrix (chemical analysis)
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Type IV collagen
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Objective: To observe the changes of extracellular matrix in type Ⅱ cells and thickness of basement membrane beneath type Ⅱ cells in old rats. Methods: Inmmunocytochemistry and image analysis were used to measure the relative contents of laminin and fibronectin in type Ⅱ cells in young (1 2 months), adult (8 9 months) and old (25 28 months) rats. Results: There were less laminin, more fibronectin in type Ⅱ cells and thicker basement membrane beneath type Ⅱ cells in old rats. Conclusion: These results suggest the altered metabolism of extracellular matrix from type Ⅱ cells in old rats, which may result in abnormal recovery of alveoli after being injured.
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