A chemostat for growing higher plant cells in single cell suspension cultures

Several techniques employing mechanical, chemical, or enzymatic methods have been suggested for the production of essentially single-cell plant suspension cultures. If single-cell cultures can be obtained, the effects of the media composition on growth can be unambiguously determined. Additionally, such cultures would be amenable to optical and electronic methods for rapidly determining cell mass, number, and volume and could easily be used in experiments on continuous cultivation. Most methods to produce single-cell cultures have been applied to only one or two species. In this paper, these techniques are compared when extended to cultures of Paul's Scarlet Rose (Rosa sp.) cells and soybean cells (Glycine max L.). It is concluded that no technique will generally give sustained disaggregation without affecting the apparent biochemical state of the culture.

Suspension culture

Suspension

Plant cell

Generality

10.1139/b78-304

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Citations (24)

Continuous culture of plant cells using the chemostat principle

Advances in biochemical engineering, biotechnology (1980)

Graham Wilson

Chemostat

Limiting

Plant cell

10.1007/3540098070_6

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Citations (31)

A chemostat for growing higher plant cells in single cell suspension cultures

Experimental Cell Research (1971)

Wolfgang Kurz

Chemostat

Suspension culture

Plant cell

Suspension

10.1016/0014-4827(71)90104-2

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Citations (39)

Phosphodiesterases of tobacco cell cultures and the intact plant

Phytochemistry (1977)

Hideaki Shinshi Kunio Katō

Suspension culture

Plant cell

10.1016/0031-9422(71)85065-3

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Citations (5)

AN-P40 Intracellular ribonucleotide pools as a tool for monitoring the physiological state of plant cell suspension culture of Vitis vinifera in response to temperature change(Section I Animal and Plant Cell Cultures)

Journal of Bioscience and Bioengineering (2009)

Hao Jiang Xiuxiu Ge Wei Zhang Christopher M. M. Franco

Suspension culture

Plant cell

Suspension

Source

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Citations (0)

Secondary Products from Plant Cell Cultures

Biotechnology Set (2001)

Jochen Berlin

The prelims comprise: Introduction Some General Conclusions and Suggestions Based on the Present Biotechnological Impact of Plant Cell Cultures as Producers Secondary Product Formation in Suspension Cultures Secondary Product Formation in Hairy Root Cultures Plant Tissue Cultures as a Source of New Chemicals? Biotransformations with Cultured Plant Cells Metabolic Engineering of Secondary Pathways in Cultured Cells Conclusions and Outlook

Plant cell

Suspension culture

Secondary metabolism

Hairy Root Culture

Metabolic Engineering

Plant tissue culture

10.1002/9783527620999.ch13g

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Citations (26)

Initiation, growth and cryopreservation of plant cell suspension cultures

Nature Protocols (2011)

Natali Rianika Mustafa Ward de Winter F. van Iren Robert Verpoorte

Suspension culture

Callus

Plant cell

Suspension

10.1038/nprot.2010.144

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Citations (201)

[The possible use of PKZh-type devices (liquid purity control devices) in the analysis of cell suspensions].

PubMed (1988)

Ivanov Nr Osin Ab Sokolov Ve Tupikin Dv Shub Gm

PKZh means "device for liquid purity control". A possibility is considered to use the native PKZh type device for carrying out quantitative analyses of cellular suspension components, for routine bacterial suspension, agglutinated bacterial suspension and erythrocyte suspension. The flowing photometric principle of particle recording, used in the device, allows to analyse biological suspensions with small amounts of components. The device provides a differential count of some cells and their conglomerates in six dimensional ranges, within the frames of 1-25 micron or higher. The time consumption for one sample analysis is 10-15 seconds.

Suspension

Suspension culture

Particle (ecology)

Source

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Citations (0)

Secondary Products from Plant Cell Cultures

Bio/Technology (1997)

Jochen Berlin

Plant cell

Suspension culture

Secondary metabolism