In Vitro Free Radical Scavenging Activity of Gallic Acid Isolated From Caesalpinia Decapetala Wood
34
Citation
14
Reference
10
Related Paper
Citation Trend
Abstract:
Antioxidant capacities and phenolic contents of Gallic Acid isolated from plant Caesalpinia decapetala were investigated. Caesalpinia decapetala is a plant used to treat burns, biliousness and stomach disorders. The antioxidant activity was estimated by the ABTS and DPPH methods. The total phenolic was measured using Folin-Ciocalteu assay. Identification of isolated Gallic Acid was also performed by reverse-phase high-performance liquid chromatography (RP-HPLC) using Cosmosil C18 column (150mm × 4.6mm, 5μm particle). The mobile phase was a mixture of ethyl acetate: ethanol: water (1:5:4, v/v/v) delivered at a flow rate of 1.0 mL min-1. The total phenolic content was found to be 4.31% (w/w). Isolation of Gallic Acid with optimum yield was performed using a mixture of solvents (Ethanol: Water 65:35). Isolated Gallic Acid showed significant in vitro free radical scavenging activity in both model; but in ABTS assay significant % inhibition of free radical was observed as compared to DPPH assay. Research concluded that Caesalpinia decapetala extract can be used as potent antioxidant which can play vital role against the diseases like neurodegenerative disorders, inflammation, viral infections and gastric ulcer.Keywords:
ABTS
Caesalpinia
In this study, it was aimed to investigate total phenolic, antioxidant and antiradical activities of water and ethanolic extracts of the Wiedemannia orientalis (W. orientalis). In order to investigate the antiradical capacities, DPPH and ABTS radical scavenging activates were employed in the evaluation process. For the evaluation of antioxidant capacities, activities of phosphomolybdenum assay, reducing power and metal chelating were investigated. In the evaluations, the amounts of total phenolics were determined to be 11.95±0.02-17.27±0.09 mg Gallic acid equivalents (GAE)/g in W. orientalis extracts, respectively. Additionally, the amount of reducing power and total antioxidant capacity of leaf ethanol extract of W. orientalis were determined to be higher compared to the other extracts of W. orientalis. While the highest activity was observed in flower ethanol extract on the DPPH radical scavenging activity, flower water extract demonstrated better results in metal chelating activity. In the ABTS radical chelating activity, no significant differences were observed. As a result, it was suggested in our study that extracts of W. orientalis could be regarded as a natural and alternative source in pharmacology and medicine and food sectors. Such results can be put to use in pharmaceutical formulations and may lead to the developments of new human drugs from this medicinal plant.
ABTS
Cite
Citations (2)
Antioxidant capacities and phenolic contents of Gallic Acid isolated from plant Caesalpinia decapetala were investigated. Caesalpinia decapetala is a plant used to treat burns, biliousness and stomach disorders. The antioxidant activity was estimated by the ABTS and DPPH methods. The total phenolic was measured using Folin-Ciocalteu assay. Identification of isolated Gallic Acid was also performed by reverse-phase high-performance liquid chromatography (RP-HPLC) using Cosmosil C18 column (150mm × 4.6mm, 5μm particle). The mobile phase was a mixture of ethyl acetate: ethanol: water (1:5:4, v/v/v) delivered at a flow rate of 1.0 mL min-1. The total phenolic content was found to be 4.31% (w/w). Isolation of Gallic Acid with optimum yield was performed using a mixture of solvents (Ethanol: Water 65:35). Isolated Gallic Acid showed significant in vitro free radical scavenging activity in both model; but in ABTS assay significant % inhibition of free radical was observed as compared to DPPH assay. Research concluded that Caesalpinia decapetala extract can be used as potent antioxidant which can play vital role against the diseases like neurodegenerative disorders, inflammation, viral infections and gastric ulcer.
ABTS
Caesalpinia
Cite
Citations (34)
Objective:To study the antioxidant activity of different extracts of Uraria lagopodioides.Method:The total flavonoids content of ethyl acetate extract,acetone extract and 95% ethanol extract from U.lagopodioides by UV methods.Their antioxidant activities were investigated in present study with different doses(0.2-1.2 g·L-1),employing various methods established in vitro systems,such as the scavenging activities on ABTS+ radical and DPPH radical,and reducing power.Result: The total flavonoids content of EAE,AE and EE was 74.0,65.7,84.2 g·kg-1,respectively.These plants possessed good scavenging activities on ABTS·+radical,DPPH· radical and reducing power activities,and the most outstanding one was found to be the ethanol extract,the IC50 value of EE in these three essays was 0.52,1.36,4.88 g·L-1,respectively.Conclusion: This study showed that all the extracts could be employed as natural antioxidants and health care products.
ABTS
Cite
Citations (0)
Objective To analyze the antioxidant activity of the extracts from Scutellaria barbata D.Don(S.barbata) with ethanol and different polar solvent. Methods The flavones were segregated from S.barbata with ethanol,and then extracted with ligroin,ethyl acetate,n-butyl alcohol,respectively.Absorption spectrum of the extract was scanned from 200 to 750 nm.ABTS+,hydroxy radical and DPPH were measured to analyze the antioxidant activity. Results The extract from S.barbata had activities to scavenge ABTS+,hydroxy radical and DPPH significantly.The extracts from different polar solvent had different antioxidant activities. Conclusion The extract from S.barbata has antioxidant activity.The extracts with different polar solvent respectively can affect the antioxidant activity significantly.
ABTS
Flavones
Cite
Citations (1)
In the present study, the phenolic compounds were prepared using ultrasonic-aid extraction from sugar beet molasses (SBM). Gallic acid (GA), cyanidin-3-O-glucoside chloride (CGC) and epicatechin (EP) were produced after column chromatography from the extraction, and further detected using NMR, QTOF-MS and ESI-MS/MS. The three compounds exhibited strong antioxidant activities including DPPH radical scavenging activities, ABTS radical scavenging activities and ORAC values. GA showed the strongest antioxidant activity. Antitumor activities significantly increased in a dose-dependent manner. In particular, the CGC had growth inhibitory activities of 94.86, 87.27 and 67.13 % against the human colon (CACO-2), hepatocellular (HepG2) and breast (MCF-7) carcinoma cell lines, respectively, at the highest concentration of 400 μg/mL of the extracts. These results suggest that the three compounds are key chemical compositions valuable for preparing functional foods in the food industry. The results suggested that SBM is a natural source of antioxidant and antitumor agents for preparing functional foods.
ABTS
Functional food
Cite
Citations (55)
This work developed a paper-based device for simultaneous determination of multiple antioxidant activity assays including the cupric reducing antioxidant capacity (CUPRAC) assay, the 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) assay, and 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulphonate) radical cation (ABTS) assay. The device composed of a central sample zone connected to four detection zones to accommodate three antioxidant assays and a sample blank measurement. Antioxidant activity analysis was achieved by dropping the samples onto the sample zone to flow to the detection zones containing the stored reagents for each antioxidant assay making the change in color that was measured using image J software. The analysis of gallic acid antioxidant standard with CUPRAC, ABTS, and DPPH assay gave the calibration curve in the linear ranges of 1-6 mM, 20-150 µM, and 3-13 mM, respectively, the relative standard deviation from the repetitive analysis of gallic acid at the concentrations in the linear range are 0.70-1.61%, 0.91-4.04% and 1.39-4.91% (n=5), respectively, and a limit of detection of 1 mM 1.10 µM and 1.30 mM, respectively. These preliminary results indicated that the developed paper-based device provided for the analysis of multiple antioxidant assays at the same time with low analysis time and cost, low reagent consumption and is promising to use for antioxidant activity in real samples. Keywords: multi-assay analysis, antioxidant activity, antioxidant, paper-based devices
ABTS
Quantitative Analysis
Cite
Citations (0)
ABTS
Cite
Citations (0)
Fruit of Ficus pumila L.was extracted with various concentration(20%～95%) ethanol solution using ultrasonic at room temperature.The antioxidant activities of different extracts were evaluated by reducing power assay,DPPH assay,TEAC assay and FRAP assay,the total phenolics contents of the extracts were measured by Folin-Ciocalteu method.The results demonstrated that the antioxidant activities of different concentration ethanol extracts showed varying degrees of efficacy in four assays(reducing power,scavenging capacity on DPPH· and ABTS·+,the total antioxidant capacity).The concentration of ethanol solution significantly affected the antioxidant activities of extracts.Antioxidant activities of different ethanol extracts were descending in the following order: 80%,95%,60%,40%,20% ethanol,in which,80% ethanol extracts had the highest antioxidant activities,the absorbance value at 700 nm was 1.04,half scavenging efficiency concentration for DPPH· was 2.20 mg·mL-1,the value of TEAC and FRAP were 2.36,157.1,respectively.The results were in accordance with the highest total phenolics content(2.50%) of 80% ethanol extract.
ABTS
Absorbance
Cite
Citations (1)
The purpose of this study was to examine biological activities, including total contents of polyphenol, antioxidant activities, inhibitory activities of tyrosinase, and protective effect against oxidative stress in the HepG2 cells of ethanol extracts from wheat sprout. The antioxidant activity of extracts was determined by ABTS and DPPH radical scavenging activities. Ethanol extracts were tested using different ethanol concentrations (0%, 30%, 50%, 80% and 95%, respectively). The highest amount of total polyphenol was extracted by 50% and 80% ethanol which was 26.3 and 26.8 mg gallic acid equivalents/g sample, respectively. High levels of ABTS and DPPH radical scavenging activity were found in 50% ethanol (26.7 and 15.0 mg TEAC/g sample, respectively) and 80% (24.3 and 16.1 mg TEAC/g sample, respectively) ethanol extracts. Also, 50% and 80% ethanol extracts indicated higher inhibitory activities of tyrosinase compared with other extracts. In the cell-based assay, pre-treatment of the HepG2 cells with wheat sprout extracts prevented the cell damage induced by TBHP (tert-butyl hydroperoxide). The results of this study indicate that wheat sprout has significantly higher diverse biological activities and apparently has significant health benefits.
ABTS
Cite
Citations (0)
Due to the adverse effects of synthetic antioxidants, there is a great desire to use
herbs because of its antioxidant properties. This study was performed to examine the antioxidant effects
of (Pimpinella affinis) as an alternative to synthetic antioxidants.
Materials & Methods: Total phenolic, contents were measured with folin ciocalteu method. The
antioxidant capacity of the extract and essential oil was assessed by DPPH (2,2-diphenyl-1-
picrylhydrazyl) radical-scavenging activity and compared to synthetic antioxidant BHT. In addition,
antioxidant capacity of extracts was also analyzed with ABTS (acid sulphonic-6-ethylbenzthiazoline-3-
azinobis, 2, 2, cation radical method. Data were analyzed using Duncan's multiple tests and the analysis
was carried out using SPSS.
Results: In testing percent of inhibition of free radicals (DPPH), the extract showed a significant
inhibitory effect compared to the essential oil (p<0.05). Both extract and essential oil also showed iron
reducing effect that was affected by the increase in the concentration. In ABTS test, most antiradical
activity related to the concentration of 4 mg/ml with 24% and 92% inhibition, respectively when
compared with BHT showed less inhibitory effect. And finally total phenolic content of essential oil and
extract was 53.14±4.25 and 37.68±1.12 mg Gallic acid/g extract, respectively.
Conclusion: The results of this study showed that extract and essential oil of Pimpinella affinis have
considerable antioxidant activity compared to synthetic antioxidant (BHT), and after conducting more
comprehensive studies can be used in food and pharmaceutical industries.
ABTS
Cite
Citations (2)