Low-copy-number plasmid-cloning vectors amplifiable by derepression of an inserted foreign promoter

Journal Article An F factor based cloning system for large DNA fragments Get access Fumie Hosoda, Fumie Hosoda 1Department of Immunology and Virology, Saitama Cancer Center Research InstituteIna-machi, Kitadachi-gun, Saitama 3622Biology Division, National Cancer Center Research InstituteTsukiji 5–1–1, chuo-ku, Tokyo 104 Search for other works by this author on: Oxford Academic PubMed Google Scholar Susumu Nishimura, Susumu Nishimura 2Biology Division, National Cancer Center Research InstituteTsukiji 5–1–1, chuo-ku, Tokyo 104 Search for other works by this author on: Oxford Academic PubMed Google Scholar Hisao Uchida, Hisao Uchida 3Department of Bioscience, Teikyo UniversityNagaoka-cho, Utsunomiya 320, Japan Search for other works by this author on: Oxford Academic PubMed Google Scholar Misao Ohki Misao Ohki 1Department of Immunology and Virology, Saitama Cancer Center Research InstituteIna-machi, Kitadachi-gun, Saitama 3622Biology Division, National Cancer Center Research InstituteTsukiji 5–1–1, chuo-ku, Tokyo 104 Search for other works by this author on: Oxford Academic PubMed Google Scholar Nucleic Acids Research, Volume 18, Issue 13, 11 July 1990, Pages 3863–3869, https://doi.org/10.1093/nar/18.13.3863 Published: 11 July 1990 Article history Received: 28 March 1990 Revision received: 30 May 1990 Accepted: 30 May 1990 Published: 11 July 1990

Center (category theory)

10.1093/nar/18.13.3863

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Citations (39)

Cloning and Transformation with Plasmid Vectors

Cold Spring Harbor Protocols (2021)

Michael R. Green Joseph Sambrook

Plasmids occupy a place of honor in molecular cloning: They were used in the first recombinant DNA experiments and, 40 or more years later, they remain as the carriage horses of molecular cloning. After almost half a century of sequential improvement in design, today's plasmid vectors are available in huge variety, are often optimized for specific purposes, and bear only passing resemblance to their forebears. Here, various features of plasmid vectors and methods for transforming E. coli cells are introduced.

Cloning (programming)

Cloning vector

Carriage

Multiple cloning site

In vitro recombination

T-DNA Binary system

10.1101/pdb.top101170

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Citations (30)

Low-copy-number plasmid-cloning vectors amplifiable by derepression of an inserted foreign promoter

Gene (1984)

Jens Erik Løve Larsen Kenn Gerdes Janice Light Søren Molin

Derepression

Cloning vector

T-DNA Binary system

Plasmid preparation

Low copy number

In vitro recombination

Cloning (programming)

Multiple cloning site

10.1016/0378-1119(84)90086-6

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Citations (200)

Functional properties of the pOV13 plasmid as a vector for DNA cloning in a broad spectrum of gram negative bacteria

Molecular Genetics Microbiology and Virology (Russian) (1990)

Zakharenko Vi Gorelov Vn Seliutina Df Kulakov IuK Nenashev Av

The birepliconed plasmid pOV13 possesses all the properties of a vector for DNA cloning in a broad host range of bacterial cells. pOV13 is transfered by transformation and stably inherited by Escherichia coli, Brucella, Pseudomonas cells determining the resistance to streptomycin, tetrocycline and kanamycin in these bacteria. The plasmid pOV13 is a multicopy plasmid optimal in replication capacity (23kb). The plasmid carries single sites for some restriction endonucleases that are used for DNA cloning, including some restriction sites in antibiotic resistance genes. The examples of DNA cloning with the selection of recombinant clones by the insertional inactivation of kanamycin or tetracycline resistance and expression of the cloned DNAs are presented.

Kanamycin

Cloning vector

Cloning (programming)

Multiple cloning site

Plasmid preparation

T-DNA Binary system

In vitro recombination

Source

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Citations (3)

Plasmid Cloning Vectors

Springer eBooks (2018)