Observation of the mica surface by atomic force microscopy
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Finite element numerical simulations have been carried out to demonstrate that by the dispersing of a small amount of exfoliated muscovite mica platelets in a solid polymer one can considerably reduce and even match the thermal expansion coefficients of metal and polymer components of hybrid polymer–metal structures. In practice, such mismatch reduction may lead to the extension of the service life of hybrid polymer–metal structures. Computer models comprised of a random dispersion of fully aligned round muscovite mica platelets have been studied. It was found that the decrease of the thermal expansion for such nanocomposites is controlled by the product of the aspect ratio and the volume fraction of the mineral platelets, and that the dependence can be accurately described by a stretched exponential master curve, thus considerably facilitating the task of designing mica–polymer nanocomposites with tailored thermal expansion.
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Abstract In this study, the topography of human topoisomerase I (TOPO I) on mica surfaces in air and in liquid has been studied by atomic force microscopy (AFM). The average height of TOPO I on mica surface in air measured by AFM was 2.59±0.32 nm. After adsorption of the 0.3 U/µl TOPO I on mica surfaces for 2 h, and then imaged in liquid by AFM, well‐separated single TOPO I was observed. The average height of TOPO I on mica surfaces in liquid measured by AFM was 2.93±0.42 nm. After adsorption of the 4 U/µl TOPO I on mica surfaces for 1.5 h, TOPO I monolayer can be formed. The produced TOPO I monolayer on mica was flat and exhibited good stability. SCANNING 31: 160–166, 2009. © 2009 Wiley Periodicals, Inc.
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Trinitrotoluene
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Abstract The imaging process with atomic force microscopy (AFM) in air is influenced by many factors, such as tip size and geometry, scan speed, ambient humidity, etc. In an ambient environment the imaging can be affected greatly by the humidity of the atmosphere because of the formation of the meniscus along the tip‐sample interface. The capillary force between the tip and the sample is generally large enough to make it difficult to obtain a high‐resolution image, therefore it is necessary to eliminate this capillary in order to obtain improved images. To this end, we modified tips with CH 3 ‐terminated organosilane to make them hydrophobic. With such tips we obtained (in AFM/LFM mode) improved images of soft materials such as the water layer and the self‐assembled monolayer (SAM). For octadecantrichlorosilane (OTS) SAM chemisorbed on mica, high‐resolution lattice‐resolved images also were collected. The results demonstrated that these hydrophobic tips were suitable for obtaining improved images. Copyright © 2001 John Wiley & Sons, Ltd.
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Self-assembled monolayer
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The structure of the Paired Helical filaments (PHF), a polymer of the microtubule associated protein tau, has been studied by Atomic Force Microscopy (AFM) and by cryoelectron microscopy. Mica and graphite were used as substrates in the AFM analysis with no differences in the results. A banding pattern of 8–12 nm width within the helical structure is found when detailed analysis of the data is performed. High AFM resolution images obtained by using an ultra sharp tip confirm the previous results and suggest that the structures observed are compatible with a helical ribbon made up of two parallel strands. These results were confirmed by cryoelectron microscopy experiments.
Characterization
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