Enhanced Immunohistochemical Detection of Ki-67 and p53 Proteins in Fresh and Archival Astrocytoma Samples with Autoclave Pretreatment
0
Citation
15
Reference
10
Related Paper
Abstract:
The Ki-67 labeling index and p53 overexpression determined with immunohistochemistry are both useful indicators of the cellular proliferative potential of brain tumors. We compared the immunohistochemical expression of Ki-67 and p53 proteins in samples of 20 astrocytomas obtained from 19 patients which had been pretreated with three different methods of antigen activation: 95°C heating, 121°C autoclaving, and exposure to target unmasking fluid. The Ki-67 labeling index of specimens pretreated with autoclaving and 95°C heating was positively correlated with the degree of malignancy of astro-cytomas (simple regression analysis, p<0.002 for autoclaving and p<0.03 for 95°C heating) . p53 overexpression was identified in 7 astrocytomas (2 cases of low-grade astrocytomas, 2 cases of anaplastic astrocytomas, and 3 cases of glioblastoma multif ormes) ; however, p53 expression was not correlated with the degree of malignancy of astrocytomas.Keywords:
Anaplastic astrocytoma
Ki-67
Antigen retrieval
Anaplastic astrocytoma
Tumor progression
Cite
Citations (110)
Objective To make use of atomic force microscope to detect the difference and change of II/ WHO,III/WHO in the surface structures of cell membrance,then to provide help and guide for the clinical differential diagnosis between Astrocytoma and Anaplastic astrocytoma.Methods Between Astrocytoma and Anaplastic astrocytoma,we choose 30 examples from each.10 cells each example was chosen randomly and scanned with AFM using tapping mode in the range of 1 μm,the surface of cell membranes being measured in terms of rough,peak value,as well as difference of surface area.We observed the Frame changes in the two groups to find out the morphologic features.Results We found out the surface of cell membrane in the two groups had significant differences on rough,peak value,and difference of surface area.The Astrocytoma cells were significantly lower than Anaplastic astrocytoma cells(P0.05).Conclusions In our experiment,we can observe the Astrocytoma and Anaplastic astrocytoma cells with AFM,then make a differential diagnosis with the data of the surface of cell membranes.
Anaplastic astrocytoma
Cite
Citations (0)
Objective To investigate the expression and significance of DR4 and DR5 in human anaplastic astrocytoma.Methods The expression of DR4 and DR5 was determined by immunohistochemistry and in situ hybridization in 24 samples of anaplastic astrocytoma and 16 samples of normal brain tissue.Results DR was expressed highly in all anaplastic astrocytoma samples,while its expression in part of normal brain tissue was low.DR protein expression in anaplastic astrocytoma tissue was significantly higher than that in normal brain tissue(P(0.01)).There was no significant difference between the expression of DR mRNA in normal brain tissue and that in anaplastic astrocytoma tissue(P0.05).Conclusions High DR expression is prevalent in anaplastic astrocytoma tissue and this may contribute to the apoptosis-inducing therapy of anaplastic astrocytoma.The difference in expression of DR protein in normal brain tissue and in anaplastic astrocytoma may be one of the mechamisms of selective induction of apoptosis by TRAIL.
Anaplastic astrocytoma
Brain tissue
Human brain
Cite
Citations (0)
BACKGROUND The prognosis of pediatric patients with nonpilocytic astrocytoma, and in particular those with anaplastic astrocytoma, is somewhat unpredictable. This study used MIB-1 monoclonal antibody, a proliferative marker that can be used in formalin fixed paraffin embedded tissues, to study nonpilocytic pediatric astrocytoma. METHODS Astrocytoma, anaplastic astrocytoma, and glioblastoma specimens excised from a total of 101 pediatric patients during the period from January 1975 to September 1996 were retrieved from the authors' surgical pathology file. Histologic grading of the specimens was performed based on a modified Ringertz system. The proliferative potential of the tumors was estimated by using the MIB-1 labeling index (LI), which was evaluated with morphologic grades of tumors and survival of the patients. RESULTS Of the 101 patients, 34 had astrocytoma, 33 had anaplastic astrocytoma, and 34 had glioblastoma. Their mean survival times were 165.2 ± 14.9 months (mean ± standard error; SE), 46.1 ± 9.9 months, and 21.8 ± 5.6 months, respectively. The mean MIB-1 LI of different tumor grades were as follows: astrocytoma, 3.9 ± 4.3 (mean ± standard deviation; range, 0.0-21.6); anaplastic astrocytoma, 24.3 ± 15.6 (range, 1.7-62.8); and glioblastoma, 35.9 ± 16.4 (range, 7.36-63.3). The mean survival of the entire group of patients with LIs ≤ 11 was 173.2 ± 12.2 months (mean ± SE), and the mean survival of those with LIs > 11 was 20.3 ± 4.1 months. The survival of anaplastic astrocytoma patients with LIs ≤ 11 was similar to that of astrocytoma patients, whereas the survival of anaplastic astrocytoma patients with LI > 11 was similar to that of patients with glioblastoma. CONCLUSIONS The results of the current study show that histopathologic grading can predict the outcome for patients with astrocytomas and glioblastomas, whereas MIB-1 LI can separate better and worse prognostic groups in patients with anaplastic astrocytoma. Cancer 1998;82:2459-2466. © 1998 American Cancer Society.
Anaplastic astrocytoma
Grading (engineering)
Cite
Citations (27)
Three-tiered system dividing supratentorial astrocytic neoplasms into the astrocytoma, anaplastic (malignant) astrocytoma and the glioblastoma multiforme has been widely used. However, the pathology of anaplastic astrocytoma is defined in different ways according to different classifications. A total of 42 biopsy specimens from 35 cases diagnosed as anaplastic astrocytoma were reviewed pathologically and their features were correlated with a follow-up clinical study to discuss the prognostic usefulness of the subdivision of anaplastic astrocytoma. In WHO classification, anaplastic astrocytoma is defined as "astrocytoma containing areas of anaplasia". Follow-up study of 7 cases with the histology as such revealed that 5 cases had survived more than one year and seven months. The other 28 cases showed a varied histology and were subclassified into an astrocytoma in which moderately anaplastic cells are found throughout the tumor, an astrocytoma formed by anaplastic fusiform cells, an astrocytoma composed of predominantly rounded anaplastic cells, and a pleomorphic astrocytoma with or without intracytoplasmic hyaline inclusions. A follow-up study of cases with these types of astrocytoma disclosed death in 15 cases within one year and 7 months following the first surgery and that three cases displayed typical histological features of glioblastoma at autopsy. It is considered that there would be a considerable overlap between the group of anaplastic astrocytoma and that of glioblastoma, if we use the term "anaplastic astrocytoma" in a broader category.
Anaplasia
Anaplastic astrocytoma
Pleomorphic xanthoastrocytoma
Histology
Cite
Citations (0)
Cite
Citations (1)
Abnormalities of the p53 gene are the most common molecular change in human cancer. In the central nervous system, mutant p53 gene is frequently identified in the tumors with astrocytic differentiation. To investigate the relation between histologic subtypes and p53 protein overexpression, we examined 81 cases of astrocytic neoplasms (24 benign astrocytoma, 28 anaplastic astrocytoma and 29 glioblastoma multiforme) using the standard immunohistochemical method. All were formalin-fixed and paraffin-embedded tissue. The p53 immunoreactivity was found in 4/24 benign astrocytoma, 18/28 anaplastic astrocytoma, 22/29 glioblastoma multiforme. The degree of immunoreactivity closely correlated with histologic subtypes (p < 0.001). Overall p53 protein expression was most frequently detected in glioblastoma multiforme, but strong immunoreactivity (3+) was more frequently found in the anaplastic astrocytoma than in glioblastoma multiforme. Although the frequency of p53 protein expression is low, 4 benign astrocytoma showed distinct nuclear staining. In conclusion the malignant progression of astrocytic neoplasms may be associated with increasing expression of p53 protein.
Anaplastic astrocytoma
P53 protein
Cite
Citations (5)
Anaplastic astrocytoma
Phosphoprotein
Cite
Citations (19)
Background: Astrocytic tumors are the most common primary tumors of the central nervous system. Several grading systems are used to grade astrocytomas. The most widely used system is the World Health Organization (WHO) classification (1979, 1993, 2000, and 2007) that grades astrocytomas (I-IV) based on cytological atypia, mitotic activity, vascular proliferation, and necrosis: pilocytic astrocytoma (grade I), diffuse astrocytoma (grade II), anaplastic astrocytoma (grade III), and glioblastoma (grade IV).Objectives: The aim of this study is to evaluate p53 over expression , Ki-67 expression in astrocytomas and Correlate these two markers with histologic grade of astrocytomas.Methods: Formalin fixed, paraffin-embedded blocks from 40 patients with brain astrocytoma included in this retrospective study. LSAB (Labeled Strept-Avidin, Biotin) method was employed for immunohistochemical detection of Ki – 67 and P53.Results: P53 was detected in (25%) of the cases and was significantly positively correlated with grade IV. Ki-67 labeling index was (>5%) in (50%) of the cases. Both biomarkers were positively correlated with each other, and the grade of astrocytoma; however, Ki67 is a better marker for differentiating (diagnostic marker) between the grades of astrocytoma than p53.Conclusion: P53 overexpression and ki-67 expression plays an important role in pathogenesis of astrocytoma evolution, as they positively associated with higher tumor grade.
Anaplastic astrocytoma
Ki-67
Pilocytic astrocytoma
Grading (engineering)
Cite
Citations (0)