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    Reduction in omega-3 fatty acids by UV-B irradiation in microalgae
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    Keywords:
    Isochrysis galbana
    Artemia salina
    Docosapentaenoic acid
    Phaeodactylum tricornutum
    Phaeodactylum tricornutum
    Isochrysis galbana
    Nannochloropsis
    Citations (39)
    Effects of initial cell density on interspecific growth interaction between Isochrysis galbana and Phaeodactylum tricornutum is studied through experiment by co-culture method.The results show that the different initial cell densities have apparent effects on the relation of interspecific growth competition between I.galbana and P.tricornutum.At three different inoculative proportions,P.tricornutum always dominates in the competition with I.galbana and exhibits inhibition to the growth of I.galbana.Moreover,with increased amount of inoculums,the interspecific dominance of P.tricornutum and the inhibition on the growth of I.galbana are found to be more significant.Only at the inoculation proportion for I:Q = 4:1,the growth of P.tricornutum is also inhibited by I.galbana even though Heterosigma akashiwo is still in dominance in the competition.
    Phaeodactylum tricornutum
    Isochrysis galbana
    Dominance (genetics)
    Citations (0)
    Six species of algae, Chlorella marina Butcher, Dunaliella tertiolecta Butcher, Isochrysis galbana Parke, Monochrysis lutheri Droop, Phaeodactylum tricornutum Bohlin and Hemiselmis virescens Droop were fed to oyster larvae in bacteria-free culture. In addition, cultures of Chlorella stigmatophora Butcher, Isochrysis galbana and Phaeodactylum tricornutum containing bacteria were also used. Cultures of galbana containing bacteria were used as controls in all series of experiments. In each series various densities of food, expressed both as cell numbers and as packed cell volume, were fed to the larvae of Ostrea edulis L. for 22–46 h and the change in the mean size of the larval population was used as the index of the value of the food. The experiments with Chlorella confirmed previous work that organisms of this genus have little value as food for larvae. With Dunaliella and Phaeodactylum growth was better at low food densities with some (but not all) batches of larvae compared with Isochrysis. The comparison of cultures of Isochrysis and Phaeodactylum with and without bacteria showed no consistent improved growth with either food. Monochrysis had about the same food value as Isochrysis. With Hemiselmis some growth was obtained but not as much as with Isochrysis.
    Phaeodactylum tricornutum
    Isochrysis galbana
    Ostrea edulis
    Dunaliella
    Dunaliella salina
    Citations (72)
    3011 strains from three algae species Phaeodactylum tricornutum,Platymonas subcordiformis and Isochrysis galbana were cultured under autotrophic and amphitrophic conditions and their growth and contents of docosahexaenoic acid (DHA),and eicosapentaenoic acid (EPA) were determined. The results showed that Phaeodactylum tricornutum and Isochrysis galbana grew rapidly and had high levels of EPA and DHA. On the constrary, the growth of Platymonas subcordiformis in the heterotrophic culture was comparatively lower with the composition of EPA and DHA decreasing. According to the results, it might come to the conclusions that the amounts of EPA and DHA were very high in Phaeodactylum tricornutum and Isochrysis galbana and profitable for marine culture.
    Phaeodactylum tricornutum
    Isochrysis galbana
    Autotroph
    Citations (0)
    In present study,the effects of temperature,light and acidity on the expression of Omega-3 fatty acid desaturase of 3 species of marine microalgae including Phaeodactylum tricornutum,Chlorella vulgaris and Isochrysis galbana were detected.The results revealed that the expression of Omega-3 fatty acid desaturase of 3 species was down-regulated following treatment with lower temperature at 10 or 15 ℃,while the transcripts of the gene were up-regulated after treatment at 25 or 30 ℃(Phaeodactylum tricornutum was excluded).An increased expression of Omega-3 fatty acid desaturase was also found when 3 species of marine microalgae were treated with light at intensity of 5 000 lx for 48 or 72 h.The results also demonstrated that the acidity produced effects on the expression of Omega-3 fatty acid desaturase of the microalgaes in association with the species.Results of culturing in pH 5-9 showed that the acidity had a greater effects on Phaeodactylum tricornutum,whereas a moderate effects on Isochrysis galbana and a smaller effects on Chlorella vulgaris.
    Phaeodactylum tricornutum
    Chlorella vulgaris
    Isochrysis galbana
    Fatty acid desaturase
    Citations (0)
    The effects of two algae (Phaeodactylum tricornutum and Isochrysis galbana) on reproduction of copepod (Calanus sinicus) were investigated in laboratory. The results showed that the average egg production of Calanus sinicus fed with diatom (Phaeodactylum tricornutum) was 0.24±0.07/d, with the survival rate of female being relatively low. In contrast, relatively high egg production (7.844±1.12/d ) and survival rate appeared in Calanus sinicus fed with flagellate (Isochrysis galbana). In addition, the egg hatching rates of Calanus sinicus fed with Phaeodactylum tricornutum were lower than those of Calanus sinicus fed with Isochrysis galbana either in algal suspension or in filtered seawater. It was evident that diatom Phaeodactylum tricornutum could produce some inhibitory effect on both fecundity and hatching of Calanus sinicus, compared to Isochrysis galbana. Through fatty acid composition analysis, it was found that Phaeodactylum tricornutum was inferior in contents of polyunsaturated fatty acids (PUFAs) and some specific fatty acids(ω3,ω6) which are necessary to copepod reproduction.
    Phaeodactylum tricornutum
    Isochrysis galbana
    Calanus finmarchicus
    Thalassiosira weissflogii
    Calanus
    Citations (0)