Differences of reproduction of Calanus sinicus fed with two species of algae
0
Citation
0
Reference
20
Related Paper
Abstract:
The effects of two algae (Phaeodactylum tricornutum and Isochrysis galbana) on reproduction of copepod (Calanus sinicus) were investigated in laboratory. The results showed that the average egg production of Calanus sinicus fed with diatom (Phaeodactylum tricornutum) was 0.24±0.07/d, with the survival rate of female being relatively low. In contrast, relatively high egg production (7.844±1.12/d ) and survival rate appeared in Calanus sinicus fed with flagellate (Isochrysis galbana). In addition, the egg hatching rates of Calanus sinicus fed with Phaeodactylum tricornutum were lower than those of Calanus sinicus fed with Isochrysis galbana either in algal suspension or in filtered seawater. It was evident that diatom Phaeodactylum tricornutum could produce some inhibitory effect on both fecundity and hatching of Calanus sinicus, compared to Isochrysis galbana. Through fatty acid composition analysis, it was found that Phaeodactylum tricornutum was inferior in contents of polyunsaturated fatty acids (PUFAs) and some specific fatty acids(ω3,ω6) which are necessary to copepod reproduction.Keywords:
Phaeodactylum tricornutum
Isochrysis galbana
Calanus finmarchicus
Thalassiosira weissflogii
Calanus
Cite
Effect of Prorocentrum donghaiense on feeding and digestive enzyme activity of marine planktonic copepod Calanus sinicus, a key species of Yellow Sea and East China Sea, was studied using single and mixed culture methods under controlled laboratory conditions. The results show that: (1) C. sinicus takes some P. donghaiense with the maximum ingestion rate (IR) of 930 cells/(ind·h), and the IR is closely related with the microalga density. Differently, the clearance rate (CR) decreases monotonically with microalga density increasing during the whole experiment. (2) The IRs of C. sinicus on Isochrysis galbana and P. donghaiense in mixed cultures are lower than those of two microalga species in single culture. (3) The digestive enzyme activity of C. sinicus changes with microalga species and density. The activity of laminarinase is higher than those of maltase and cellobiase, and cellobiase has little lower activity than those of other two. The result also shows that the microalga species affects the digestive enzyme activity. C. sinicus fed with P. donghaiense showed relatively higher digestive enzyme activity than those fed with I. galbana (P0.05).
Isochrysis galbana
Digestive enzyme
Dinoflagellate
Calanus
Cite
Citations (1)
Isochrysis galbana
Phaeodactylum tricornutum
Cite
Citations (5)
The trumpet shell Charonia sauliae is an endangered and valuable species with potential for aquaculture. For artificial propagation of C. sauliae, the effects of three different food microalgae on the development, growth, and survival rate of the larvae and spat were investigated. For the larval feeding experiments, we utilized six microalgae species as food sources, namely Pavlova lutheri, Tetraselmis suecica, Nannochloris oculata, Isochrysis galbana, Chaetoceros calcitrans, and Phaeodactylum tricornutum; for the larval and spat growth and survival experiments, we utilized T. suecica, C. calcitrans, and P. tricornutum. The results showed that the temporal digestion index (TDI) for the veliger larvae was significantly different for C. sauliae fed the different microalgae species (P < 0.05), that the T. suecica, C. calcitrans, and P. tricornutum cultivars were better suited for larval consumption (P < 0.05), and that the growth and survival of the larvae and spat were significantly influenced by food type, specifically P. tricornutum (P < 0.05). Further research is needed to evaluate the effects of other microalgae species, different algal concentrations, and biochemical composition on the growth and survival of C. sauliae.
Phaeodactylum tricornutum
Tetraselmis suecica
Isochrysis galbana
Tetraselmis
Chaetoceros
Broodstock
Cite
Citations (1)
Acartia tonsa
Thalassiosira weissflogii
Isochrysis galbana
Dinoflagellate
Acartia
Haptophyte
Cite
Citations (87)
Tetraselmis suecica
Isochrysis galbana
Nannochloropsis
Phaeodactylum tricornutum
Cite
Citations (16)
Brachionus
Phaeodactylum tricornutum
Isochrysis galbana
Cite
Citations (75)
Tisbe furcata, Nitokra lacustris, Calanus finmarchicus and Calanus glacialis were reared for several months in the laboratory prior to feeding selected diets. Copepods were fed Isochrysis galbana, Tetraselmis sp., Dunaliella tertiolecta, Thalassiosira pseudonana, Rhodomonas sp., Rhodomonas lens and Oxyrrhis marina individually or in combination. Oxyrrhis marina itself was fed up to four of the algae species. The lipid class and fatty acid composition of the copepods was determined and compared with each other, the diets and with wild C. finmarchicus, C. glacialis and Pseudocalanus sp. Cultured O. marina, T. furcata and N. lacustris had greater proportions of essential 22:6ω3 or 20:5ω3 acids than in their diets suggesting nutritional enrichment through synthesis or preferential retention. Lower proportions of 18:3ω3 than in their diets fed individually or in combination suggests desaturation and elongation of 18:3ω3. Stable isotope analysis of the heterotroph samples and their dietary sources revealed similar values for 18:3ω3 in each dietary pair and different ones for 22:6ω3 and 20:5ω3 supporting derivation from precursors rather than trophic magnification. Principal components analysis of copepods and their diets showed cultured Calanus spp. to have the most similar fatty acid composition to their diet indicating little modification or sequestration of polyunsaturated fatty acids.
Isochrysis galbana
Calanus finmarchicus
Calanus
Tetraselmis suecica
Cite
Citations (65)
Six species of algae, Chlorella marina Butcher, Dunaliella tertiolecta Butcher, Isochrysis galbana Parke, Monochrysis lutheri Droop, Phaeodactylum tricornutum Bohlin and Hemiselmis virescens Droop were fed to oyster larvae in bacteria-free culture. In addition, cultures of Chlorella stigmatophora Butcher, Isochrysis galbana and Phaeodactylum tricornutum containing bacteria were also used. Cultures of galbana containing bacteria were used as controls in all series of experiments. In each series various densities of food, expressed both as cell numbers and as packed cell volume, were fed to the larvae of Ostrea edulis L. for 22–46 h and the change in the mean size of the larval population was used as the index of the value of the food. The experiments with Chlorella confirmed previous work that organisms of this genus have little value as food for larvae. With Dunaliella and Phaeodactylum growth was better at low food densities with some (but not all) batches of larvae compared with Isochrysis. The comparison of cultures of Isochrysis and Phaeodactylum with and without bacteria showed no consistent improved growth with either food. Monochrysis had about the same food value as Isochrysis. With Hemiselmis some growth was obtained but not as much as with Isochrysis.
Phaeodactylum tricornutum
Isochrysis galbana
Ostrea edulis
Dunaliella
Dunaliella salina
Cite
Citations (72)
We compared the development and fatty acid content of the harpacticoid copepods Tachidius discipes and Tisbe sp. fed with different microalgal species (Dunaliella tertiolecta, Rhodomonas sp., Phaeodactylum tricornutum, Isochrysis galbana and a concentrate of Pavlova sp.), which differed in cell size and fatty acid composition. Tisbe could develop in 11 days with every alga to the same average stage, whereas Tachidius developed poorly when fed with Isochrysis and Dunaliella. Feeding with Phaeodactylum resulted in a fast development of both copepods at low algal concentrations. However, reproduction was higher with Rhodomonas as food than with the other algae. Fatty acid compositions of copepods were influenced by their food source, but both were able to convert docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) from precursors. Tachidius fed with Rhodomonas or Phaeodactylum was closest to the DHA/EPA/arachidonic acid (ARA) ratio of 10 : 5 : 1 considered optimal for some marine fish larvae. Tachidius showed similar development and reproduction capacity as Tisbe, but requested higher absolute fatty acid contents in the diet. Tisbe was superior in the utilization of bacteria as additional food source and the bioconversion of precursor fatty acids. Phaeodactylum and Rhodomonas are recommendable food sources for both copepod species, but Phaeodactylum is more easily cultured.
Phaeodactylum tricornutum
Isochrysis galbana
Nannochloropsis
Cite
Citations (42)
2 algae - Chaetoceros muelleri (CM) and Scrippsiella trochoidea (ST) were investigated for the influence on the reproduction of Calanus sinicus. HS-SPME/GC-MS was developed for the analysis of aldehydes produced by phytoplankton. The results showed that, compared with ST, CM induced inhibitory effects on female survival, egg production and egg hatching for Calanus sinicus. By the analysis of aldehydes, 5 kinds of aldehydes were detected in CM, whereas only 2 kinds of aldehydes ST contained. Furthermore, the total content of aldehydes in Chaetoceros muelleri (397.86 10 -3 μg /g) was significantly higher than that in Scrippsiella trochoidea (82.84 10 -3 μg /g). Therefore, the evidence indicated that aldehydes produced by Chaetoceros muelleri caused potential negative effect on the reproduction of Calanus sinicus.
Chaetoceros
Cite
Citations (0)