PROCESS OPTIMIZATION IN ULTRAFILTRATION: FLUX-TIME CONSIDERATIONS IN THE PURIFICATION OF MACROMOLECULES
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Abstract An empirical model was developed to describe the flux of a complex milk protein suspension under ultrafiltration and diafiltration conditions. Flux decreased during ultrafiltration, but increased during diafiltration as the permeable solids concentration decreased. The "gel" model based on film theory was modified to describe the flux in terms of both retained and permeable solutes. The least-time processing strategy for a given end product concentration was also modelled and identified for this protein system. In general, ultrafiltration followed by diafiltration is best if protein purification is the goal.Keywords:
Diafiltration
Ultrafiltration (renal)
Downstream processing
Ultrafiltration (renal)
Diafiltration
Downstream processing
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Ultrafiltration (renal)
Diafiltration
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Abstract An empirical model was developed to describe the flux of a complex milk protein suspension under ultrafiltration and diafiltration conditions. Flux decreased during ultrafiltration, but increased during diafiltration as the permeable solids concentration decreased. The "gel" model based on film theory was modified to describe the flux in terms of both retained and permeable solutes. The least-time processing strategy for a given end product concentration was also modelled and identified for this protein system. In general, ultrafiltration followed by diafiltration is best if protein purification is the goal.
Diafiltration
Ultrafiltration (renal)
Downstream processing
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Diafiltration
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Expanded bed adsorption
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Ultrafiltration (renal)
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Ultrafiltration (renal)
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Wet-milling
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Ultrafiltration with diafiltration is a state-of-the-art process used to separate solutions containing high and low molecular weight solutes. The focus of this paper is on the different concepts of ultrafiltration with diafiltration – batch, continuous and counter-current – and the potential realization of diafiltration on an industrial scale in pharmaceutical biotechnology. Two case studies will be discussed from both technical and economical aspects.
Diafiltration
Ultrafiltration (renal)
Industrial biotechnology
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Purification of the alkaloids by ultrafiltration using hollow fibre membrane from hydrochloric acid extract of Sophora alopecuroides L was investigated. Ultrafiltration followed by discontinuous diafiltration was carried out to examine the result of purification of alkaloids. Meanwhile, the effects of membrane pore size, operating pressure, pretreatment of the extract and pH value on ultrafiltration performances were also researched. The results showed that the ultrafiltration with discontinuous diafiltration could effectively remove proteins and other impurities. The recovery ratio of alkaloid from the permeate was high.
Diafiltration
Ultrafiltration (renal)
Hydrochloric acid
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Polyquaternium-6 (PQ6 ) 作为水溶性的聚合物被用于阴离子在 ultrafiltration 前钨(VI ) 形成的 complexing。钨(VI )-PQ6 建筑群被 polysulfone 保留在 complexation-ultrafiltration 过程的空纤维 ultrafiltration 膜。象聚合物金属比率(PMR ) 那样的各种各样的操作参数的效果， pH 和氯化物离子集中在上渗入流动(J) 和钨拒绝系数(R) 被调查。包括改革聚合物的集中， decomplexation， diafiltration 和复用的四个实验的集成被执行。在集中的过程， J 慢慢地衰退， R 在 3 的 PMR 和 7 的 pH 是大约 1。在 retentate 的钨集中与体积集中因素线性地增加。钨与膜高效地被集中。集中的 retentate 为 decomplexation 进一步被使用。在 50 mgL1 的氯化物离子集中到达 decomplexation 平衡拿大约 6 min。钨(VI ) 的 decomplexation 百分比 -PQ6 建筑群活动范围 56.1% 。在 diafiltration 过程，钨(VI ) 能被使用改革 PQ6 的 50 mgL1 氯化物离子答案，和纯化有效地提取是可欣然接受地令人满意的。改革 PQ6 被用来在各种各样的 pH 价值绑钨(VI ) 。改革 PQ6 的有约束力的能力接近新鲜 PQ6 的，并且有约束力的能力的恢复百分比比 90% 高。
Diafiltration
Ultrafiltration (renal)
Polysulfone
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