Fractionation of whey proteins and caseinomacropeptide by means of enzymatic crosslinking and membrane separation techniques
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Diafiltration
Tissue transglutaminase
Ultrafiltration (renal)
Abstract Cross‐flow microfiltration (CMF) and diafiltration were used to concentrate and purify recombinant Brain‐Derived Neutrophic Factor (rBDNF) inclusion bodies from an E. coli cell suspension and a homogenized E. coli cell suspension (homogenate/lysate). Although these processes have been tested industrially in pilot scale with conventional linear membrane microfiltration modules, their performances were severely limited due to membrane fouling. The purpose of this work was to determine whether Dean vortex microfiltration with controlled centrifugal instabilities (Dean vortices produced in helical flow) could be used to improve filtration performance over that observed with conventional linear cross‐flow microfiltration (CMF). For the microfiltration experiments with the feeds containing cell and homogenate suspensions, improvements in flux of about 50 and 70%, respectively, were obtained with the helical module as compared with that obtained with the linear module. For diafiltration with the homogenate suspension as feed, solute transport (as measured by mass) was from 100 to 40% higher after 40 and 100 min, respectively, with the helical module as compared with that obtained with the linear module. In the presence of the neutral surfactant, Tween 20, solute transport for diafiltration was at least 25 times higher during the first 10 min of operation and 100% higher after 300 min with the helical module as compared with that obtained with the linear module. Clearly, improved filtration performance, a purer and more concentrated product, and substantial savings can be expected with the new Dean vortex filters.
Diafiltration
Filtration (mathematics)
Cross-flow filtration
Suspension
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Abstract An empirical model was developed to describe the flux of a complex milk protein suspension under ultrafiltration and diafiltration conditions. Flux decreased during ultrafiltration, but increased during diafiltration as the permeable solids concentration decreased. The "gel" model based on film theory was modified to describe the flux in terms of both retained and permeable solutes. The least-time processing strategy for a given end product concentration was also modelled and identified for this protein system. In general, ultrafiltration followed by diafiltration is best if protein purification is the goal.
Diafiltration
Ultrafiltration (renal)
Downstream processing
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Membranes Membrane Module and Operation Permeate Flux in Ultrafiltration Protein Transmission Through UF Membranes Selectivity of Solute Separation in UF Protein Concentration Diafiltration of Protein Solutions Protein Fractionation Using Ultrafiltration New Developments.
Diafiltration
Ultrafiltration (renal)
Protein purification
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This chapter contains sections titled: Introduction Materials, Module Configurations, and Manufacturers Microfiltration/Ultrafiltration Pretreatment Membrane Applications Membrane Fouling and Cleaning Integrated Membrane Systems (MF or UF + RO or NF) Backwash Water Reuse, Treatment, and Disposal References
Ultrafiltration (renal)
Membrane Fouling
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Microfiltration and ultrafiltration have gained rapid acceptance as processes that provide reliable and high‐level particle, turbidity, and microorganism removal.
Ultrafiltration (renal)
Turbidity
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Diafiltration
Cross-flow filtration
Particle (ecology)
Particle deposition
Sieve (category theory)
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Ultrafiltration with diafiltration is a state-of-the-art process used to separate solutions containing high and low molecular weight solutes. The focus of this paper is on the different concepts of ultrafiltration with diafiltration – batch, continuous and counter-current – and the potential realization of diafiltration on an industrial scale in pharmaceutical biotechnology. Two case studies will be discussed from both technical and economical aspects.
Diafiltration
Ultrafiltration (renal)
Industrial biotechnology
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This paper explains the use of ultrafiltration and microfiltration membranes in the treatment of drinking water. The basic types of ultrafiltration and microfiltration membranes, their characteristics, and the most important manufacturers of membranes for ultrafiltration and microfiltration are listed. Membrane filtration due to its advantages such as quality of treated water, the simplicity of the process, reduced use of chemicals, is replacing the now dominant technologies such as flocculation and ion exchange. Number of membrane treatment plants is constantly growing, so it can be said that they represent the future of membrane processes in water treatment.
Ultrafiltration (renal)
Filtration (mathematics)
Membrane Technology
Nanofiltration
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Microfiltration (MF) of whey or whey protein concentrate (WPC) generates two streams; the permeate which is further processed to whey protein isolate (WPI) and the retentate which is a natural source of milk fat globule membrane (MFGM) material. The MF retentate is an underutilized coproduct, with the yield and chemical composition of this MF retentate being influenced by the partitioning of whey components during MF and diafiltration (DF) of whey in the production of WPI, yet very little research has been published on this topic. Therefore, the current study investigated component partitioning during cold MF and DF of WPC feed in the production of WPI. Results showed significant differences (P<0.05) in the partitioning of components in the WPC feed. Specifically, 60.1, 50.2, 6.32 and 75.7% of total solids, protein, fat, and ash were partitioned into the permeate stream, respectively. Phospholipids (PLs) represented 25% of total fat in WPC feed, which was concentrated to 41.4% in DF retentate, with all PLs analysed being retained in the retentate samples. Sodium dodecyl sulfate polyacrylamide gel electrophoretic analysis indicated that MFGM-associated proteins (i.e., xanthine oxidase and butyrophilin) were all enriched in the MF retentate. The particle size in MF retentate was significantly larger (P<0.05) than the MF and DF permeate stream with values of 450, 7.87, 3.55 nm, respectively, providing further evidence of the retention of aggregated whey proteins in the MF retentate. The results of the current study provide an in-depth understanding of component partitioning during cold MF and DF of WPC feed, helping to support the development of higher value-added ingredients from the coproduct of WPI production.
Diafiltration
Ultrafiltration (renal)
Component (thermodynamics)
Whey protein isolate
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Polyquaternium-6 (PQ6 ) 作为水溶性的聚合物被用于阴离子在 ultrafiltration 前钨(VI ) 形成的 complexing。钨(VI )-PQ6 建筑群被 polysulfone 保留在 complexation-ultrafiltration 过程的空纤维 ultrafiltration 膜。象聚合物金属比率(PMR ) 那样的各种各样的操作参数的效果， pH 和氯化物离子集中在上渗入流动(J) 和钨拒绝系数(R) 被调查。包括改革聚合物的集中， decomplexation， diafiltration 和复用的四个实验的集成被执行。在集中的过程， J 慢慢地衰退， R 在 3 的 PMR 和 7 的 pH 是大约 1。在 retentate 的钨集中与体积集中因素线性地增加。钨与膜高效地被集中。集中的 retentate 为 decomplexation 进一步被使用。在 50 mgL1 的氯化物离子集中到达 decomplexation 平衡拿大约 6 min。钨(VI ) 的 decomplexation 百分比 -PQ6 建筑群活动范围 56.1% 。在 diafiltration 过程，钨(VI ) 能被使用改革 PQ6 的 50 mgL1 氯化物离子答案，和纯化有效地提取是可欣然接受地令人满意的。改革 PQ6 被用来在各种各样的 pH 价值绑钨(VI ) 。改革 PQ6 的有约束力的能力接近新鲜 PQ6 的，并且有约束力的能力的恢复百分比比 90% 高。
Diafiltration
Ultrafiltration (renal)
Polysulfone
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