Regeneration of intergeneric somatic hybrids by protoplast fusion betweenOnobrychis viciaefolia andMedicago sativa
6
Citation
9
Reference
10
Related Paper
Citation Trend
Keywords:
Protoplast
Somatic fusion
Cell fusion
Discoveries in the 1960s and 1970s showed that plant protoplasts could be freed from their walls by digestion with fungal enzymes (), grown in culture, and regenerated back into intact plants (). This work opened the way for protoplast fusion and somatic hybridization in plants (). In the ensuing 20 years, there have been substantial improvements in protoplast fusion and culture, and sophisticated approaches for the selection and characterization of somatic hybrids have been developed. As a result, in the 1990s, protoplast fusion has come of age, and fusion-derived somatic hybrids are being evaluated in practical plant breeding programs (, , .
Protoplast
Electrofusion
Somatic fusion
Cell fusion
Cite
Citations (4)
Protoplast
Cite
Citations (2)
Protoplast
Somatic fusion
Heterokaryon
Auxotrophy
Cell fusion
Cite
Citations (67)
Introduction Why Fuse Isolated Plant Protoplasts? Procedures for Protoplast Fusion Chemical Fusion of Protoplasts Electrical Fusion of Protoplasts Products of Protoplast Fusion and the Selection of Somatic Hybrid Tissues and Plants Somatic Hybrid Tissues and Plants Homokaryons and Heterokaryons Procedures for Selecting Hybrid Cells and Plants Characterization of Somatic Hybrid Plants Generation of Asymmetrical Hybrids and Cybrids by Protoplast Fusion Gametosomatic Hybridization Transfer of Unique Traits by Protoplast Fusion Concluding Remarks Bibliography
Protoplast
Somatic fusion
Heterokaryon
Cell fusion
Cite
Citations (5)
Protoplast
Cell fusion
Auxotrophy
Heterokaryon
Petri dish
Bimolecular fluorescence complementation
Cite
Citations (4)
Protoplast
Somatic fusion
Cell fusion
Plant cell
Cite
Citations (3)
Protoplast
Somatic fusion
Cell fusion
Cite
Citations (6)
Mesophyll protoplasts of Nicouana plwnbaginifoJia (PP) and N. sylvestris (SS) were mixed at equal density, treated with polyethylene glycol (PEG) and then cultured in medium with no selection pressure against parental cells. Cytological, morphological, and biochemical studies showed that about 78%of plants regenerated from protoplast fusion were somatic hybrids. The ploidy levels of somatic hybrids varied, and the frequencies of plants with PPSS, PPPPSS, and PPPPSSSS genome constitutions were 84.8%, 8.7%, and 6.5%, respectively. Aneuploid numbers and chromosome structural changes were common in somatic hybrids. The- successful recovery of somatic hybrids in the absence of artificial selection is attributed to a high viability of the fusion products following PEG treatment and a fast rate of growth and development of hybrid cells during in vitro culture.
Protoplast
Somatic fusion
Cell fusion
Cite
Citations (2)
Protoplast
Somatic fusion
Datura
Callus
Datura stramonium
Cell fusion
Cite
Citations (66)
Interspecific hybridization by fusion of leaf protoplasts of Nicotiana glauca (GG) and N. langsdorffii (LL) was confirmed and extended. Enzymatic digestion of leaf tissues to obtain protoplats was followed by fusion with the aid of polyethylene glycol. The hybrid calli were selected by their better growth on defined culture media. Mature hybrid plants were identified by their morphology and tumor formation. Cytological examination revealed a range in chromosome numbers from 56 to 64 rather than the amphiploid GGLL number of 42. About 75 percent of the hybrids were fertile. The potential range in combining widely disparate genotypes by somatic cell fusion was demonstrated by fusing tobacco GGLL protoplasts with human HeLa cells. The HeLa nucleus was observed inside the plant protoplasts, thus forming an interkingdom heterokaryon.
Protoplast
Heterokaryon
Somatic fusion
Cell fusion
HeLa
Cite
Citations (0)