Comparisons of clinical features of HLA‐DRB1*07 positive and negative vitiligo patients in Chinese Han population
D. HuYunqing RenKunju ZhuYuanyuan LvHui ChengZhongtang ZhangYong LiShengfu HeJiapeng TangJianzhu LiuYan LinYuwei SunX. ZuoG. ChenLimei SunSen YangX.‐J. Zhang
22
Citation
25
Reference
10
Related Paper
Citation Trend
Abstract:
Human leucocyte antigen (HLA)-II alleles have been found to be associated with vitiligo in different populations, and several studies also suggested that HLA class II alleles/haplotypes were associated with a different type vitiligo. Of HLA class II alleles, DRB1*07 has consistently shown a positive association with vitiligo in Chinese Han population.To further explore the relationship between DRB1*07 and vitiligo and to evaluate the DRB1*07 effect on the clinical features of vitiligo in Chinese Han population.This study investigated DRB1*07 allele distribution in 1178 unrelated Chinese vitiligo patients and 1743 healthy controls using polymerase chain reaction/sequence specific primer method and observed clinical differences between DRB1*07 positive and DRB1*07 negative patients.The analysis of the 1178 cases and 1743 controls revealed a highly association between DRB1*07 allele and vitiligo [odds ratio (OR) = 1.97, P = 2.13 × 10(-17) ]. DRB1*07 positive patients had early disease onset (OR = 1.49, P = 0.001), higher frequency of family history (OR = 1.44, P = 0.006) compared with DRB1*07 negative patients.The DRB1*07 showed significant association with vitiligo in the study population. This study confirmed that DRB1*07 positive patients had some obvious clinical differences from DRB1*07 negative patients in the Chinese Han population.Keywords:
Vitiligo
HLA-DRB1
Vitiligo
Medical History
Cite
Citations (69)
Human leucocyte antigen (HLA)-II alleles have been found to be associated with vitiligo in different populations, and several studies also suggested that HLA class II alleles/haplotypes were associated with a different type vitiligo. Of HLA class II alleles, DRB1*07 has consistently shown a positive association with vitiligo in Chinese Han population.To further explore the relationship between DRB1*07 and vitiligo and to evaluate the DRB1*07 effect on the clinical features of vitiligo in Chinese Han population.This study investigated DRB1*07 allele distribution in 1178 unrelated Chinese vitiligo patients and 1743 healthy controls using polymerase chain reaction/sequence specific primer method and observed clinical differences between DRB1*07 positive and DRB1*07 negative patients.The analysis of the 1178 cases and 1743 controls revealed a highly association between DRB1*07 allele and vitiligo [odds ratio (OR) = 1.97, P = 2.13 × 10(-17) ]. DRB1*07 positive patients had early disease onset (OR = 1.49, P = 0.001), higher frequency of family history (OR = 1.44, P = 0.006) compared with DRB1*07 negative patients.The DRB1*07 showed significant association with vitiligo in the study population. This study confirmed that DRB1*07 positive patients had some obvious clinical differences from DRB1*07 negative patients in the Chinese Han population.
Vitiligo
HLA-DRB1
Cite
Citations (22)
Objective To investigate the association of HLA DR with vitiligo in Han ethnical Chinese in Northern China. Methods By employing PCR/SSO procedure 91 vitiligo patients in Northern China were studied for HLA DRB1 alleles and compared to the 262 normal controls. Results There was a significant increase in HLA DRB1＊ 070x and HLA DRB1＊ 1201,2 but a significant decrease in HLA DRB1＊ 0301,2(17,18) alleles in vitiligo patients. HLA DRB1＊ 070x and HLA DRB1＊ 1201,2 were significantly increased in the scattered type of vitiligo while HLA DRB1＊ 070x was significantly increased in the acral type. HLA DRB1＊ 070x allele significantly increased in the patients with positive family history of vitiligo. HLA DRB1＊ 070x was significantly associated with adulthood onset cases while HLA DRB1＊ 070x and HLA DRB1＊ 1201,2 were increased in patients with childhood onset. Conclusions These results may provide clues to reveal the susceptibility gene of vitiligo in China and as well as the immunogenetic mechanisms of the disease.
Vitiligo
HLA-DRB1
Cite
Citations (0)
Objective. The Human Leukocyte Antigen (HLA) Class II is the major histocompatibility complex surface glycoproteins of humans responsible for presenting exogenous antigenic peptides which help direct specificity of immune response. In immune-cell therapy, the HLA allelic variants are of particular importance as they determine the successful activation of target cells that results to a desired therapeutic response. However, HLA Class II exhibits high polymorphism and has variable distribution in population, constituting these so-called allelic variants. Specifically, the HLA Class II DRB1 is considered the predominant locus among Filipinos. This research aimed to identify the presence of HLA Class II DRB1 allelic variants in the stem cell samples of ten (10) Filipino cancer patients by reverse transcription polymerase chain reaction (RT-PCR) amplification.Method. This study employed a PCR-based HLA Class II typing to identify the HLA Class II DRB1 allelic variant in Filipino cancer patients. Design of forward and reverse primers for HLA Class II DRB1, optimization of PCR conditions for amplifying HLA Class II DRB1, and identification of HLA Class II DRB1 allelic variants from samples by sequencing and database comparison were conducted.Results. PCR optimization showed that optimum annealing temperature for HLA DRB1 was 58.8°C with 1 mM MgCl2. PCR amplification of HLA DRB1 from ten anonymized cancer patient samples and DNA sequencing revealed that Patients 1, 2, 5, 8, 9, and 10 harbor HLA DRB1 allelic variants, particularly, the HLA DRB1*04:06:01, HLA DRB1*12:01:01, HLA DRB1*0813, HLA DRB1*04:05:01, HLA DRB1*09:01:02, and HLA DRB1*16:02:01, allelic variants, respectively.Conclusion. Using the designed primers and optimized RT-PCR protocol, HLA information derived from six out of ten patient samples can be used for further applications in developing personalized or generic antigenic peptides such as dendritic cell cancer vaccine.
HLA-DRB1
Cite
Citations (0)
Objective To investigate HLA-A,-B and -DRB1 allele and HLA-A-B,B-DRB1,A-B-DRB1 haplotype frequencies in the northwest Chinese Han population. Methods The authors investigated the HLA-A, -B, -DRB1 allele and haplotype in a northwest Chinese Han population based on 62 families and 101 individuals by use of PCR-sequence specific oligonucleotide probes (PCR-SSOP) DNA typing methods. Results Fifteen alleles for the locus HLA-A, 28 alleles for the HLA-B locus and 13 alleles for the HLA-DRB1 were detected. The results showed that the most frequent HLA alleles found were A02 (0.3244), B13 (0.1200), and DRB1*15 (0.1400). Allele frequencies of more than 10% for HLA antigens were A02, A11, A24, B13, B15, B40, DRB1*04, DRB1*07, DRB1*09, DRB1*15. In the analysis of HLA haplotypes, 122 kinds of HLA-A-B haplotypes and 147 kinds HLA-B-DRB1 haplotypes were found. Two hundred and seventy-eight kinds of HLA-A-B-DRB1 haplotypes were found, comprising 61.78% (278/450) of total theoretical haplotypes. Eighty-three kinds of HLA-A-B-DRB1 haplotypes were shown to have at least two same haplotypes, comprising 18.44% (83/450) of total theoretical haplotypes. The most common HLA-A-B-DRB1 haplotypes were A30-B13-DRB1*07, A02-B46-DRB1*09, A01-B37-DRB1*10, A24-B15-DRB1*15, A02-B46-DRB1*08, A33-B58-DRB1*03. Conclusion The data can be used for the estimation of the probability of finding haplotypically identical, related or unrelated bone marrow donor for an individual patient, and forensic and paternity tests to estimate the frequency of a DNA profile or anthropologic research.
HLA-A
HLA-DRB1
Cite
Citations (0)
Objective To investigate family history, sex, age of onset, disease severity and environmental predisposing factors in vitiligo patients. Methods Eight hundred and fifteen vitiligo patients were investigated by questionnaires. Patients with family history were compared with those without such history. SPSS 10.0 software package was applied for data analysis. Results Of 815 vitiligo probands, 128 had family history and 687 did not. The heritability rate was 15.7%. Compared with general population, vitiligo probands with affected fathers or mothers had a relative risk (RR) of 132 or 72, respectively. The RRs of those with affected first-degree relatives varied from 12 to 28. There was no significant difference of mean age of onset and disease severity between patients of paternal inheritance and maternal inheritance. No significant difference was found regarding sex and mean age of onset between the groups with and without family history. However, the patients with family history were more likely to have scattered, bilateral distributed and progressive vitiligo. Of the environmental predisposing factors, the mean daily sun-exposure time was closely related to vitiligo in patients with family history. Conclusion Genetic factors may play an important role in the pathogenesis and disease severity of vitiligo.
Vitiligo
Proband
Family aggregation
Cite
Citations (0)
To investigate HLA-A, -B and -DRB1 allele and HLA-A-B, B-DRB1, A-B-DRB1 haplotype frequencies in the northwest Chinese Han population.The authors investigated the HLA-A, -B, -DRB1 allele and haplotype in a northwest Chinese Han population based on 62 families and 101 individuals by use of PCR-sequence specific oligonucleotide probes(PCR-SSOP) DNA typing methods.Fifteen alleles for the locus HLA-A, 28 alleles for the HLA-B locus and 13 alleles for the HLA-DRB1 were detected. The results showed that the most frequent HLA alleles found were A02 (0.3244), B13 (0.1200), and DRB1*15 (0.1400). Allele frequencies of more than 10% for HLA antigens were A02, A11, A24, B13, B15, B40, DRB1*04, DRB1*07, DRB1*09, DRB1*15. In the analysis of HLA haplotypes, 122 kinds of HLA-A-B haplotypes and 147 kinds HLA-B-DRB1 haplotypes were found. Two hundred and seventy-eight kinds of HLA-A-B-DRB1 haplotypes were found, comprising 61.78%(278/450) of total theoretical haplotypes. Eighty-three kinds of HLA-A-B-DRB1 haplotypes were shown to have at least two same haplotypes, comprising 18.44%(83/450) of total theoretical haplotypes. The most common HLA-A-B-DRB1 haplotypes were A30-B13-DRB1*07, A02-B46-DRB1*09, A01-B37-DRB1*10, A24-B15-DRB1*15, A02-B46-DRB1*08, A33-B58-DRB1*03.The data can be used for the estimation of the probability of finding haplotypically identical, related or unrelated bone marrow donor for an individual patient, and forensic and paternity tests to estimate the frequency of a DNA profile or anthropologic research.
HLA-A
HLA-DRB1
HLA-B Antigens
Cite
Citations (3)
The Type 1 Diabetes Genetics Consortium has collected type 1 diabetic families worldwide for genetic analysis. The major genetic determinants of type 1 diabetes are alleles at the HLA-DRB1 and DQB1 loci, with both susceptible and protective DR-DQ haplotypes present in all human populations. The aim of this study is to estimate the risk conferred by specific DR-DQ haplotypes and genotypes.
HLA-DQ
HLA-DRB1
Cite
Citations (717)
Ayurveda gives a ray of hope in Vitiligo patients.In Ayurveda , Vaman &Virechanare the two major process of Panchakarma, which are very effective in Vitiligo.It helps to decrease the patches and to recovery of the normal skin colour in Vitiligo and thus improvingthe quality of life in Vitiligo patients.
Vitiligo
Cite
Citations (0)