Study of oxidation by chemiluminescence. IV. Detection of low levels of lipid hydroperoxides by chemiluminescence
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Abstract Sodium hypochlorite (NaOCl) induced decomposition of organic hydroperoxides gave strong chemiluminescence. Chemiluminescence intensity reached its maximum a few seconds after the addition of sodium hypochlorite and decreased to the background level in three min. Good linear relationships were observed between total chemiluminescence counts in three min and the amounts of hydroperoxides. This chemiluminescence method can be applied to the detection of low levels of lipid hydroperoxides.Keywords:
Sodium hypochlorite
Hypochlorite
Luminol
Chemiluminescence, a low-temperarure light emission due to a chemical reaction, is demonstrated on the reaction of luminol (5-amino-2,3-dihydrophthalazine-1,4-dione), hydrogen peroxide, potassium hexacyanoferrate(III) and alkaline hydroxide. The synthesis of luminol from common chemicals and its impressive chemiluminescence are described.
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Potassium hydroxide
Peroxide
Potassium periodate
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Development of novel high-sensitivity chemiluminescence assay for luminol using thiourea derivatives
We have screened about 100 thiourea derivatives in order to develop a sensitive chemiluminescence detection for luminol derivatives. Among these derivatives, we found a new compound, 2-(3-methylthioureido) thiazole, that could be used to measure luminol in the presence of hydrogen peroxide (H2O2). The detection limits of luminol and N-(4-aminobutyl)-N-ethylisoluminol (ABEI) were 10 fmol and 100 fmol, respectively. The mechanism of proposed chemiluminescence reaction was studied by electron spin resonance (ESR) with and without superoxide dismutase (SOD) and the addition of ethanol. The results showed that 2-(3-methylthioureido) thiazole has the ability to generate hydroxyl radical from H2O2, and produces intense chemiluminescence in the presence of luminol. The proposed novel chemiluminescence reaction for luminol and luminol derivatives was applied to a high performance liquid chromatography (HPLC) assay for amino compounds. Copyright © 1999 John Wiley & Sons, Ltd.
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Luminol
Hydroxyl radical
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Abstract— The chemiluminescence reaction of luminol has been investigated using conditions of methylene blue photosensitized oxidation. The quantum yields of chemiluminescence obtained were dependent upon temperature and the concentrations of luminol and base; and under the optimum conditions of high temperature and low luminol concentration, the value of the quantum yield approached that for the chemical reaction where the oxidant is hydrogen peroxide and catalyst. An analysis of the results suggests that it is not the primary species produced on photosensitization which is responsible for the chemiluminescent reaction, but a species produced by reaction of the primary species with base.
Luminol
Methylene blue
Quantum yield
Base (topology)
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We explored the behaviour of a series of phenolic acids used as enhancers or inhibitors of luminol chemiluminescence by three different methods to determine if behaviour was associated with phenolic acid structure and redox character. All the phenolic acids inhibited chemiluminescence when hexacyanoferrate (III) was reacted with the phenolic acids before adding luminol. The redox character of these compounds was clearly related to structure. When hexacyanoferrate(III)-luminol-O2 chemiluminescence was initiated by phenolic acid-luminol mixtures some phenolic acids behaved as enhancers of chemiluminescence, and others as inhibitors. We propose a mechanism to explain these findings. We found direct relationships between the redox character of the phenolic acids and the enhancement or inhibition of the chemiluminescence of the luminol-H2O2-peroxidase system and we propose mechanism to explain these phenomena.
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Phenolic acid
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Chloramine derivatives of amino acids induce chemiluminescence of a luminol solution. The chemiluminescence is more prolonged than the emission of luminol produced by hypochlorite. Persistent chemiluminescence also appears under the action of hypochlorite on a mixture of luminol and amino acids. It is assumed that the chemiluminescence of luminol in suspensions of stimulated phagocytes may be associated with its oxidation by chloramines.
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Abstract The oxidation of luminol with t‐butyl hypochlorite and the fluorescence and chemiluminescence of some of the products are herein reported. The fluorescence was studied in the present work as a function of pH and the spectral distribution was then recorded at the pH of maximum fluorescence intensity; the chemiluminescence spectrum was obtained under the conditions of the luminol‐chemiluminescence.
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