Evaluation of Group B Streptococcus Differential Agar for detection and isolation of Streptococcus agalactiae
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Subculture (biology)
Group B
Isolation
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Using an alkaline phosphatase-based genetic screening method, we identified a number of proteins that are potentially located on the outer surface of Group B streptococcus (Streptococcus agalactiae). In an enzyme-linked immunosorbent assay, antisera raised against two of the proteins, the streptococcal yutD homologue and a subunit of an ABC transporter, recognised clinically important serotypes of Group B streptococcus. In a neonatal rat model, purified IgG from the sera conferred significant levels of protection against a lethal challenge infection. The proteins identified show potential as protein subunit candidates for vaccines against Group B streptococcal disease in neonates.
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Streptococcus Pyogenes
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Studied was the diagnostic value of the liquid enriched nutrient medium - Streptococcus broth with kanamycin, used for the isolation of Sc. agalactiae and other mastitis streptococci in individual milk samples as well as that of blood dextrose agar with polymyxin + Staphylococcus toxin seeded after Koch for the isolation of Sc. agalactiae and other hemolytic streptococci in pooled cow milk. Direct seedings in thallium sulfate-crystal violet-B toxin blood agar (TKT agar) were used as a control. It was found that the enriched Streptococcus broth with kanamycin yields growth of 0.23 per cent more Sc. agalactiae organisms and 1.82 per cent other mastitis streptococci in individual milk samples as compared to the TKT agar. The blood dextrose agar with polymyxin seeded after Koch demonstrates fivefold more Sc. agalactiae in pooled cow milk as against the direct seedings in TKT agar, and it can be used to confirm the infection on the farms.
Isolation
Polymyxin B
Nutrient agar
Kanamycin
Chocolate agar
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ABSTRACT A beta-hemolytic Lancefield antigen A-, B-, C-, D-, F-, and G-positive Enterococcus durans strain was cultivated from the rectovaginal swab of a pregnant woman who underwent antenatal screening for Streptococcus agalactiae . The isolate raised concern as to what extent similar strains are misrecognized and lead to false diagnosis of group B streptococci.
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Subculture (biology)
Group B
Isolation
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When grown overnight on Columbia agar in an atmosphere of hydrogen and carbon dioxide, haemolytic strains of Lancefield group B streptococci (Streptococcus agalactiae) produce orange or brown pigmented colonies. This production of pigmented colonies can be used for the rapid presumptive identification of these organisms as belonging to group B without the need for grouping by serological methods.
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Disc diffusion susceptibility tests were performed on standardized broth suspensions inoculated from a four-hour subculture of a positive blood culture bottle as well as from an agar plate subculture of the same positive blood culture bottle. The zone sizes and interpretative results of both methods were compared. Of 101 isolates tested, only one organism exhibited a very major difference between the two methods (one Staphylococcus epidermidis was interpreted as susceptible to methicillin by the four-hour subculture method and resistant by the agar plate subculture method). Thirteen other isolates showed minor changes from susceptible or resistant to intermediate or from intermediate to either susceptible or resistant with only one antibiotic each. The data indicates that disc diffusion susceptibilities may be performed easily and accurately from a four-hour subculture of positive blood culture bottles.
Subculture (biology)
Blood Culture
Agar diffusion test
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A β-hemolytic Enterococcus faecalis strain agglutinating Lancefield group A, B, C, D, F, and G antisera was observed from a rectovaginal swab, in the context of antenatal screening for Streptococcus agalactiae (group B Streptococcus [GBS]). This is the first multi-Lancefield antisera-agglutinating isolate of this species, and it raised particular concern, as it may mimic GBS, leading to false reporting and useless receipt of intrapartum antibiotics.
Enterococcus faecalis
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Background: Group B Streptococcus (GBS) is an important pathogen that causes serious infections in newborns. Pregnant screening and intrapartum antibiotic prophylaxis are actually the strategies to prevent GBS disease in neonates because vaccination is under investigation. Materials and Methods: Simultaneously, 156 isolates of GBS and 156 isolates other than GBS covering 17 different species, were tested to evaluate the selectivity of a new chromogenic medium to screen GBS. Results: The new new chromogenic medium showed an excellent performance, exhibiting a very high level of inclusivity (100%) and exclusivity (96.1%).
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ABSTRACT Direct culture of rectovaginal specimens on Granada agar was compared to culture on sheep blood agar plate (SBAP) and AccuProbe detection of group B streptococcus from overnight LIM broth enhancement cultures (LIM-SBAP). Both broth-enhanced methods demonstrated excellent sensitivity (97.5% for LIM-SBAP and 93.5% for AccuProbe), while Granada agar demonstrated a sensitivity of only 40.3%.
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