A new, simple and sensitive analytical method for determination of methyldopa in pharmaceutical formulations using the 2,2-diphenyil-picrylhydrazyl
3
Citation
0
Reference
20
Related Paper
Citation Trend
Abstract:
SUMMARY. A new, simple, precise, sensitive and low-cost spectrophotometric method for methyldopa (MTD) determination in pharmaceutical preparations is described. This method is based on the reduction reaction of the free radical, 2,2-diphenyil-picrylhydrazyl (DPPH•), by the MTD. Absorbance of the resulting product is measured at 515 nm. Beer’s Law is obeyed in a concentration range of 3.36 × 10–6 a 1.34 × 10–5 mol/L methyldopa with a good correlation coefficient (r = 0.9971). No interference was observed from common excipients in formulations. The results show a simple, accurate and readily applied method to the determination of methyldopa in pharmaceutical products. The analytical results obtained for these products by the proposed method are in agreement with those of the Brazilian Pharmacopoeia procedure at 95 % confidence level.Keywords:
Pharmaceutical formulation
Absorbance
Methyldopa
Cite
A simple, accurate, sensitive and reproducible visible spectrophotometric method has been developed for the determination of Gliclazide in bulk and also in its pharmaceutical dosage form. The proposed method was based on ion-complex of the drug with Bromo Cresol Green showing absorbance maxima at 411 nm respectively. Beer's law was obeyed in the range of 50-300 μg/mL , with molar absorptivity 1.044x 103 L.mol-1cm-1, relative standard deviation of the method was less than 1% and accuracy (average recovery %) was 94. All the variables were studied to optimize the reaction conditions. No interference was observed in the presence of common pharmaceutical excipients. The validity of the methods was tested by analyzing the drug in its pharmaceutical preparations. Good recoveries were also obtained. The developed method employed was successful for the determination of Gliclazide in various pharmaceutical preparation.
Gliclazide
Molar absorptivity
Absorbance
Spectrophotometry
Relative standard deviation
Pharmaceutical formulation
Cite
Citations (0)
A simple, sensitive, rapid and accurate spectrophotometric method was developed in ultraviolet region for the estimation of Fluconazole in pure drug and pharmaceutical formulation. The drug has an absorption maximum at 260 nm and obeys Beer's Lambert law in the concentration range of 2- 10 μg/ml with correlation coefficient of 0.9999 in solvent. Excellent recovery proved that the method was sufficiently accurate. There is no interference from any common pharmaceutical additives and diluents. Results of the analysis were validated by recovery studies according to ICH Q2B guidelines . . Keywords — Fluconazole; methanol Spectrophotometry; Validation; Pharmaceutical formulations
Beer–Lambert law
Pharmaceutical formulation
Diluent
Spectrophotometry
Cite
Citations (5)
Molar absorptivity
Chromotropic acid
Terfenadine
Ion-association
Stoichiometry
Cite
Citations (10)
Simple, swift and sensitive method for Spectrophotometric determination of thiamine (VB1) in pharmaceutical tablets has been described. The proposed method is based on the reaction of thiamine (VB1) and 7-Chloro-4-nitrobenzoxadiazole (NBD-Cl) at alkaline medium (pH 10.5) to develop a deep brown adduct that bears maximum absorption at 434 nm. Beer’s law is obeyed in the concentration range 5-35 µg/ml of thiamine at the selected wavelength. Under optimized reaction conditions, the linear regression coefficients were a=0.033, b=-0.009, and r2=0.999 calculated for the general equation of the calibration curve (y = ax + b).
The limit of detection (LOD) and limit of quantification (LOQ) were found to be 0.667µg/ml and 2.020µg/ml respectively. The method has been successfully applied to the determination of thiamine (VB1) in pharmaceutical formulations, and can be used as an alternative of the existing sophisticated method used in drug laboratories and factories.
Pharmaceutical formulation
Cite
Citations (9)
Article history A simple, extraction free spectrophotometric method for the quantitative estimation of levetiracetam (LEV) in bulk drugs and pharmaceutical formulations (tablets) has been developed. The method is based on the oxidation of 2, 4- dinitrophenylhydrazine (2, 4-DNP) and coupling of the oxidized product with drugs to give intensely colored chromogen. Levetiracetam at its λmax 455 nm shows linearity in the concentration range of 30-130 µg mL -1 . The relative standard deviation for this method is 0.422%. Linear relationship with good correlation coefficients (0.995-0.996) was found between absorbance and the corresponding concentrations of the drug. The reliability and performance of the proposed method was validated statistically the percentage recovery ranged from 99.88 and 100.2. The results of analysis for this method have been validated statistically and by recovery studies.
Levetiracetam
Absorbance
Relative standard deviation
Pharmaceutical formulation
Cite
Citations (12)
A simple, sensitive, highly accurate spectrophotometric method has been developed for the determination of carvedilol in bulk and pharmaceutical formulations. The adequate drug solubility and maximum assay sensitivity was found in methanol. The absorbance of carvedilol was measured at 284 nm in the wavelength range of 200–350 nm. Beer's law was obeyed in the concentration range of 10–35 μg/mL. The slope, intercept and correlation coefficient were also calculated. The proposed method has been successfully used for the analysis of the drug in pure and its tablet dosage forms. Results of percentage recovery shows that the method was not affected by the presence of common excipients. The method was validated by sensitivity and precision which proves suitability of proposed method for the routine estimation of carvedilol in bulk and pharmaceutical formulations.
Carvedilol
Absorbance
Pharmaceutical formulation
Beer–Lambert law
Cite
Citations (10)
A simple, rapid and sensitive spectrophotometric method was developed for the determination of perindopril in pharmaceutical formulations.The proposed method is based on the reaction of amine group of drug with 2, 4 dinitrofluorobenzene in dimthylsulfoxide (DMSO) to form yellow colored product, which absorbs maximally at 410 nm.Beer's law was obeyed in the concentration range 2.5 -25 µg/mL with molar absorptivity 6.71 ×10 3 L/mol/cm.The limits of detection and quantitation of the proposed method were 0.17 and 0.52 μg/mL, respectively.The optimum experimental condition for the proposed procedure was investigated.The results of the proposed method were compared with those of Abdellatef's spectrophotometric method, which indicated excellent agreement with acceptable true bias of all samples within ±2.0%.
Perindopril
Cite
Citations (13)
A simple and sensitive spectrophotometric method has been developed for the estimation of lomefloxacin hydrochloride in its marketed formulations. The method is based on the reaction between the drug and the dichlone, in the presence of crotonaldehyde in dimethylsulfoxide, which produces a blue chromogen with absorption maximum at 645 nm. The good agreement with Beer's law was found in the concentration range of 5-100 µg/ml. The optimum reaction conditions and other analytical parameters are evaluated. Statistical comparison of the results with those of reported method shows good agreement, and indicated no significant difference in precision. The proposed method was found to be simple, accurate, and reproducible for the routine analysis of the drug in pharmaceutical dosage forms.
Lomefloxacin
Cite
Citations (9)
Abstract A simple, rapid, cost effective and extraction‐free spectrophotometric method has been developed for the determination of zolmitriptan in pharmaceutical raw and dosage forms. The method is based on the charge‐transfer reaction of zolmitriptan in acetonitrile medium with 0.2% 2,3‐dichloro‐5,6‐dicyano‐1,4‐benzoquinone to form a colored product peaking at 555 nm. Beer's law is obeyed in the concentration range 10‐250 μg mL −1 with molar absorptivity of 1.7 × 10 3 L mole −1 cm −1 . The effects of variables such as reagent concentration, time of reaction, color stability and interferences have been investigated to optimize the procedure. The results have been validated analytically and statistically. The proposed method has been successfully applied for the determination of zolmitriptan in pharmaceutical formulations. Results indicate that the method is accurate, precise and reproducible (relative standard deviation < 2%).
Zolmitriptan
Molar absorptivity
Pharmaceutical formulation
Relative standard deviation
Cite
Citations (26)
Two simple and sensitive spectrophotometric methods (A and B) for the determination of imatinib in bulk drugs and pharmaceutical formulations are described. In method A, distilled water was used as solvent and shows absorbance maximum at 251 nm. In method B, 0.1 N HCL was used as solvent and shows absorbance maximum at 230 nm. In both the methods linearity was found to be in the range of 10 -50 μg/ml; for method A (Y=0.0184 X+0.0061; r2=0.9992) and for method B (Y=0.0164 X-0.0032; r2=0.9997), respectively. These methods were tested and validated for various parameters according to USP guidelines. The quantitation limits were found to be 0.0334 and 0.0279 μg mL−1, for both the methods. The proposed methods were successfully applied for the determination of imatinib in pharmaceutical formulations. The results demonstrated that the procedure is accurate, precise and reproducible (relative standard deviation <2%), while being simple, cheap and less time consuming and can be suitably applied for the estimation of imatinib in different dosage forms.
Absorbance
Pharmaceutical formulation
Relative standard deviation
Distilled water
Cite
Citations (4)