SPECTROPHOTOMETRIC DETERMINATION OF LEVETIRACETAM USING 2, 4-DNP IN PHARMACEUTICAL DOSAGE FORM
12
Citation
0
Reference
20
Related Paper
Citation Trend
Abstract:
Article history A simple, extraction free spectrophotometric method for the quantitative estimation of levetiracetam (LEV) in bulk drugs and pharmaceutical formulations (tablets) has been developed. The method is based on the oxidation of 2, 4- dinitrophenylhydrazine (2, 4-DNP) and coupling of the oxidized product with drugs to give intensely colored chromogen. Levetiracetam at its λmax 455 nm shows linearity in the concentration range of 30-130 µg mL -1 . The relative standard deviation for this method is 0.422%. Linear relationship with good correlation coefficients (0.995-0.996) was found between absorbance and the corresponding concentrations of the drug. The reliability and performance of the proposed method was validated statistically the percentage recovery ranged from 99.88 and 100.2. The results of analysis for this method have been validated statistically and by recovery studies.Keywords:
Levetiracetam
Absorbance
Relative standard deviation
Pharmaceutical formulation
Cite
A simple, accurate, sensitive and reproducible visible spectrophotometric method has been developed for the determination of Gliclazide in bulk and also in its pharmaceutical dosage form. The proposed method was based on ion-complex of the drug with Bromo Cresol Green showing absorbance maxima at 411 nm respectively. Beer's law was obeyed in the range of 50-300 μg/mL , with molar absorptivity 1.044x 103 L.mol-1cm-1, relative standard deviation of the method was less than 1% and accuracy (average recovery %) was 94. All the variables were studied to optimize the reaction conditions. No interference was observed in the presence of common pharmaceutical excipients. The validity of the methods was tested by analyzing the drug in its pharmaceutical preparations. Good recoveries were also obtained. The developed method employed was successful for the determination of Gliclazide in various pharmaceutical preparation.
Gliclazide
Molar absorptivity
Absorbance
Spectrophotometry
Relative standard deviation
Pharmaceutical formulation
Cite
Citations (0)
A simple, accurate, specific and precise UV Spectrophotometric method has been developed for estimation of valsartan in pure and pharmaceutical formulation. The λ max of valsartan in methanol and water was found to be 248.21 nm. Methanol and water is used as diluent in equal proportion. The drug exhibited the linearity in the concentration range of 2-20μg/ml with correlation coefficient of 0.999. The % recovery of the drug for the proposed was found to be 99.2%. The limit of detection and limit of quantification was found to be 0.15% and 0.45% respectively. No interference was observed in the presence of common pharmaceutical excipient. The method was validated as per ICH guidelines. The developed method was successfully employed for the estimation of valsartan in pharmaceutical dosage form.
Diluent
Excipient
Pharmaceutical formulation
Cite
Citations (9)
Two simple and sensitive spectrophotometric methods (A and B) in visible region have been developed for the estimation of Pramipexole dihydrochloride in pure and pharmaceutical formulations. Method A and B is based on the condensation of heterocyclic amino group of Pramipexole with Para-dimethyl aminocinnamaldehyde (PDACA) and vanillin in presence of acidic media i.e. conc. Hydrochloride to form a colored chromogen with a characteristic absorption maximum at 525nm and 425nm respectively. Beer's law is obeyed in the concentration range of 50350μg/mL and 10–50μg/mL for Method A and B respectively. The results obtained with the proposed methods are in good agreement with labeled amounts when the marketed pharmaceutical formulations are analyzed. The results of analysis have been validated statistically and by recovery studies.
Pramipexole
Pharmaceutical formulation
Cite
Citations (2)
The aim of the research study was the development and validation of a simple, rapid, accurate and precise reversed-phase high performance liquid chromatography (RP-HPLC) stability-indicating method for the determination of aceclofenac in bulk and pharmaceutical dosage forms.The RP-HPLC studies was performed on the instrument Jasco HPLC system with Jasco UV 2010 photo diode array detector, ODS C18 RP-column (Intersile 250 mm × 4.6 mm; i.d. 10 μm), Rheodyne injection syringe with 20µL loop volume and windows based chrompass software was used for separation.The isocratic elution was performed using the mobile phase ratio of methanol: water (65:35 v/v) and UV detection wavelength at 263 nm.The overall run time of the analysis was 20 minutes and the flow rate was 1.0 mL/min.The RP-HPLC method developed for analysis of aceclofenac was validated as per the ICH guidelines with respect to specificity, selectivity, linearity, accuracy, precision and robustness.The linearity for developed method was observed in the concentration range of 5-50 μg/mL with the correlation coefficient (r 2 ) of 0.9992.The percentage accuracy of aceclofenac ranged from 99.40 to 100.79%.The relative standard deviation for inter-day precision was lower than 2.0%.The assay of aceclofenac was determined in tablet dosage form was found to be within limits.Aceclofenac was subjected to stress conditions such as neutral, acidic, alkaline, oxidation, and photolysis degradations as per ICH guidelines.The results of degradation studies revealed that the drug degraded a maximum (32.68%) in acidic conditions followed by alkaline conditions (15.05%).The drug was found to be resistant towards neutral, acidic and photolytic degradation conditions.
Aceclofenac
Cite
Citations (0)
Three simple, accurate, sensitive and economical spectrophotometric methods have been developed and subsequently validated for determination of duloxetine hydrochloride in bulk and pharmaceutical formulation. Method A is an extractive spectrophotometric method based on formation of ion-pair complexes of duloxetine hydrochloride with Solochrome Black T, to form pink colored chromogen, which showed a maximum absorption at 512 nm, and obeys Beer’s law in the concentration range of 2-10 μg/mL. Method B is based on the formation of charge transfer complexation reactions of duloxetine hydrochloride with picric acid to form a yellow colored chromogen, which showed maximum absorption at 420 nm and obeys Beer’s law in the concentration range of 5-25 μg/mL. Method C is a UV spectrophotometric method using isopropyl alcohol as solvent, in which absorption maxima was at 290 nm, Beer’s law obeyed in the concentration range of 5-30 μg/mL. The validation of the above methods was done and the results demonstrated that, the procedures were accurate, precise and reproducible. So, the proposed methods can be applied for the routine analysis of duloxetine hydrochloride in bulk drug and in its tablet dosage form.
Duloxetine Hydrochloride
Hydrochloride
Cite
Citations (0)
A novel, simple and economic reverse phase high performance liquid chromatographic method was developed and validated as per the ICH guidelines for the quantitative estimation of Sitagliptin Phosphate in pharmaceutical tablet dosage form with greater precision and Accurac Orthophosphoric acid (40:55:5).The eluent was monitored at 265 nm, at a flow rate of 1 mL/min and retention time was observed at 10 min.calibration curve as linear over the concentration range of 6 determination of precision was <2%.Accuracy of method was determined through recovery studies which were found to be 99.82-101.87%.The LOD and LOQ were found to be 0.05 mg/mL and 0.16 mg/mL respectively.Validation studies demonstrated that the proposed RP reproducible.Hence the proposed method can be applied for the routine quality control analysis of Sitagliptin Phosphate in bulk and Pharmaceutical tablet dosage forms.
Sitagliptin Phosphate
Retention time
Phosphate buffered saline
Cite
Citations (1)
A simple and sensitive spectrophotometric method has been developed for the estimation of lomefloxacin hydrochloride in its marketed formulations. The method is based on the reaction between the drug and the dichlone, in the presence of crotonaldehyde in dimethylsulfoxide, which produces a blue chromogen with absorption maximum at 645 nm. The good agreement with Beer's law was found in the concentration range of 5-100 µg/ml. The optimum reaction conditions and other analytical parameters are evaluated. Statistical comparison of the results with those of reported method shows good agreement, and indicated no significant difference in precision. The proposed method was found to be simple, accurate, and reproducible for the routine analysis of the drug in pharmaceutical dosage forms.
Lomefloxacin
Cite
Citations (9)
Asimple, rapid and sensitive spectrophotometric method for the determination of Ornidazole, in pure and pharmaceutical formulations,has been developed and validated. The proposed method is based on the reduction of the nitro group to amino group of the drug followed by diazotization and coupling reaction with ±- naphtol. The maximumabsorbance for the obtained red colored chromogen was found at i¬max = 521.5 nm. The experimental conditions were optimized and BeerÂÂs law was obeyed in the concentration range of1-15 µg.ml-1.Results of the analysis were validated statistically and by recovery study.
Ornidazole
Pharmaceutical formulation
Spectrophotometry
Relative standard deviation
Cite
Citations (0)
Abstract A simple, rapid, cost effective and extraction‐free spectrophotometric method has been developed for the determination of zolmitriptan in pharmaceutical raw and dosage forms. The method is based on the charge‐transfer reaction of zolmitriptan in acetonitrile medium with 0.2% 2,3‐dichloro‐5,6‐dicyano‐1,4‐benzoquinone to form a colored product peaking at 555 nm. Beer's law is obeyed in the concentration range 10‐250 μg mL −1 with molar absorptivity of 1.7 × 10 3 L mole −1 cm −1 . The effects of variables such as reagent concentration, time of reaction, color stability and interferences have been investigated to optimize the procedure. The results have been validated analytically and statistically. The proposed method has been successfully applied for the determination of zolmitriptan in pharmaceutical formulations. Results indicate that the method is accurate, precise and reproducible (relative standard deviation < 2%).
Zolmitriptan
Molar absorptivity
Pharmaceutical formulation
Relative standard deviation
Cite
Citations (26)
Levetiracetam
Therapeutic Drug Monitoring
Potassium phosphate
Quantitative Analysis
Coefficient of variation
Cite
Citations (61)