Effect of anti-P-selectin monoclonal antibody on renal ischemia/reperfusion injury in rats.
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To explore the effect of anti-P-selectin monoclonal antibody (mAb) on renal ischemia/reperfusion (I/R) injury in rats.Renal function, renal histopathological changes, plasma P-selectin levels and renal P-selectin protein and mRNA expression were studied in a renal I/R injury rat models. Biochemical measurement, ELISA, Immunohistochemistry and Nested RT-PCR were used.Renal function insufficiency and renal histopathological damage were much less in the anti-P-selectin mAb-treated group than the saline-treated group. Plasma P-selectin levels were lower and renal P-selectin protein and mRNA expression were down-regulated in the former group.Anti-P-selectin mAb might be an efficient approach for the treatment of renal I/R injury.Keywords:
P-selectin
Renal ischemia
Renal Injury
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Ulinastatin
Renal ischemia
Blood urea nitrogen
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Postconditioning (PostC), a series of brief ischemia/reperfusion (I/R) cycles at reperfusion onset, is a recently described approach to attenuate I/R injury in the heart and brain.Here, we examined its effect on acute kidney injury (AKI) induced by ischemia/reperfusion (I/R) injury in a mouse model.C57/black mice were subjected to right nephrectomy and 26-min left renal artery occlusion, and then divided into three groups: Group I, mice were only reperfused for 48 hr; Group II, mice received PostC that was initiated by three cycles of 30-s ischemia with a 30-s interval immediately after initial ischemia before reperfusion; Group III, mice were reperfused for 10 min after initial ischemia and then received the same regime of PostC prior to reperfusion.At 48 hr after reperfusion, renal function was assessed by measurement of serum creatinine and blood urea nitrogen (BUN), and tubular damage was evaluated by histology.Our results showed that I/R injury led to increased serum creatinine and BUN levels and tubular damage.However, PostC did not improve renal function, or attenuate pathological damage to tubules.These results suggest that PostC does not provide a protective effect on renal injury due to ischemia in C57/black mice with these two protocols.
Renal ischemia
Blood urea nitrogen
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Objective:To observe the protective effect of P38MAPK inhibitor against kidney dysfunction induced by ischemia/reperfusion injury in rats.Methods: Animal model of kidney ischemia/reperfusion injury was duplicated through renal artery occlusion. P38MAPK signal transduction pathway was blocked by venous injection of P38MAPK inhibitor. Renal function and cytokines were assayed.Results:The contents of TNF-α and IL-1β in kidney and plasma increased significantly with the duration of reperfusion. So did the renal function damage.P38MAPK inhibitor notably improved the renal function with the dropping of TNF-α and IL-1β level in kidney and blood. Conclusion: P38MAPK inhibitor can attenuate renal dysfunction induced by ischemia/reperfusion through depressing the production of pro-inflammatory factors.
Renal ischemia
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To explore the effect of anti-P-selectin monoclonal antibody (mAb) on renal ischemia/reperfusion (I/R) injury in rats.Renal function, renal histopathological changes, plasma P-selectin levels and renal P-selectin protein and mRNA expression were studied in a renal I/R injury rat models. Biochemical measurement, ELISA, Immunohistochemistry and Nested RT-PCR were used.Renal function insufficiency and renal histopathological damage were much less in the anti-P-selectin mAb-treated group than the saline-treated group. Plasma P-selectin levels were lower and renal P-selectin protein and mRNA expression were down-regulated in the former group.Anti-P-selectin mAb might be an efficient approach for the treatment of renal I/R injury.
P-selectin
Renal ischemia
Renal Injury
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AIM: To investigate the effect and possible mechanism of ulinastatin on renal ischemia-reperfusion injury in rats. METHODS: Male Sprague-Dawley rats were subjected to 45-min bilateral renal ischemia, treated with intravenously 12 500 U ulinastatin at 30 min prior to ischemia and at the beginning of reperfusion, compared with a nontreated group without ulinastatin and a sham-operation group without bilateral renal ischemia. After 0 h, 2 h, 6 h, 12 h, and 24 h of reperfusion, serum creatinine and blood urea nitrogen were measured for the assessment of renal function, renal sections were used for histologic grading of renal injury, for immunohistochemical localization of Bcl-2 and heat shock protein 70. Renal ultrastructure was observed through a transmission electron microscope. RESULTS: Ulinastatin significantly reduced the increase in blood urea nitrogen and creatinine produced by renal ischemia-reperfusion, suggesting an improvement in renal function. Ulinastatin reduced the histologic evidence of renal damage associated with ischemia-reperfusion and accompanied with an up-regulation in the expression of Bcl-2 protein, but it had no significent effect on the expression of HSP 70. Ulinastatin also significantly reduced kidney ultrastructure damage caused by renal ischemia-reperfusion. CONCLUSION: The protease inhibitor, ulinastatin, reduced the renal dysfunction and injury associated with ischemia-reperfusion of the kidney. The protective effect of ulinastatin might be associated with the up-regulation of Bcl-2 expression and the effect on membrane fragility.
Ulinastatin
Renal ischemia
Blood urea nitrogen
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Objective To investigate the role of anti-L-selectin monoclonal antibodies on ischemia-reperfusion injuried skin flap. Methods The rat ischemia-reperfusion injury model was established, and anti-L-selectin monoclonal antibody was applied. The skin flap survival area was measured and histological changes were observed. Results On the 7th day post-injury, the skin flap survival percentage was 100% in the sham operation group,(18.3±19.6)% in the saline control group, and (85.63±22.05)% in the anti-L-selectin group. Histologically, severer inflammatory cells infiltration, obvious tissue swelling and partial skin necrosis could be observed in the saline control group. While the inflammatory reaction and tissue necrosis was less serious in the anti-L-selectin group, almost to the similar degree as in the sham operation group. Conclusion The anti-L-selectin monoclonal antibody alleviates the ischemia-reperfusion injury, therefore increases the survival area of ischemia-reperfusion injuried skin flap.
Skin flap
Infiltration (HVAC)
P-selectin
E-selectin
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Ischemia-reperfusion (I/R) is present at various degrees in kidney transplants. Several studies suggest that renal ischemia reperfusion (RIR) can induce acute kidney injury. Liver diseases and neurological disorders related to kidney injury is a common clinical problem. Olive leaf is a significant source of bioactive phenolic compounds. They have better antioxidant capacity, anti-inflammatory and radical scavenging. In this study 50 male rats were allocated randomly into 5 groups: control (intact animals), group-1(I/R 60min+olive leaf extract), group-2 (I/R 60min), group-3(I/R 120min+olive leaf extract)and group-4(I/R 120min).The animals received 100 mg/kg olive leaf extract in0.5 ml drinking water using gavage for 28 days. Other animals received 0.5 ml normal saline by gavages. At the end of the treatment, the level of antioxidant enzymes including TAC, MDA, SOD and GPX were determined in renal tissue. Administration of olive leaf extract can significantly increase activity of TAC, GPX and SOD in group1and 3compared with group2and4. Also, MDA level in renal tissue of treated groups was significantly lower than ischemia-reperfusion groups (p<0.05). This study showed that olive leaf extract has protective effects against renal ischemic-reperfusion injury.
Renal Injury
Adult male
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ObjectiveTo discuss the mechanism of Yanshen Granules 1 in antagonism of renal ischemia and reperfusion injury.MethodThe rats (n=72) were randomly divided into the sham-operation group,control group and treatment group (each n=24). The treatment group was given Yanshen Granules 1,and the sham-operation group and control group were given same quantity of tap water. The changes of renal function indexes (BUN an Cr),tumor necrosis factor-α(TNF-α) and intercellular adhesion molecules-1 (ICAM-1) were detected after renal ischemia for 1 hour,and after reperfusion for 24 and 48 hours.ResultIn the sham-operation group there were slight expressions of TNF-α and ICAM-1 observed in renal tubular epithelial cells,while in the treatment group and control group the expressions of TNF-α and ICAM-1 increased and had significant difference after renal ischemia and reperfusion for 24 and 48 hours (P0.01). The expressions of TNF-α and ICAM-1 decreased in the treatment group compared with those in the control group,and had no significant difference after renal ischemia for 1 hour (P0.05) but had significant difference after reperfusion for 24 and 48 hours (P0.01).ConclusionYanshen Granules 1 can protect renal function and inhibit the expressions of TNF-α and ICAM-1 in rats with renal ischemia and reperfusion injury.
Renal ischemia
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Objective To observe the effect of Yansheng No. 1 granule for renal function of rat with renal ischemia reperfusion injury. Methods 72 rats were randomly divided into three groups including sham group,control group and treatment group. Rats in treatment group accepted Yansheng No. 1 granule,2g/kg.d. Rats in sham group and control group accepted corresponding tap water. Changes of BUN and Cr in rat 1h ischemia,24h and 48h of reperfusion were recorded. And renal pathology was observed. Results BUN and Cr in control group and treatment group obviously decreased in comparison with those in sham!group (P0.01). Improvement of pathological changes and repaired extent in treatment group was superior to that in sham group and control group (P0.05). Conclusion Yansheng No. 1 granule has protection on renal function of rat with renal ischemia reperfusion injury.
Renal Injury
Granule (geology)
Renal ischemia
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