The effect of in vitro competition on shoot regeneration from hypocotyl explants of Linum usitatissimum
22
Citation
30
Reference
10
Related Paper
Citation Trend
Abstract:
This study was carried out to investigate the relationship between in vitro competition and stress, and their effects on tissue culture response of Linum usitatissimum L. hypocotyl explants. Competition among explants was achieved by varying the spacing among the explants cultured. Four different culture spacing distances were used: 0.5, 1.0, 1.5, and 2.0 cm. Six weeks after culture initiation, hypocotyl fresh and dry weights, shoot regeneration percentage, shoot number per hypocotyl, regenerated shoot length, total shoot number per petri dish, and total chlorophyll content were recorded. The results showed that encouraging competition among explants by decreasing spacing among them from 2.0 cm to 1.0 cm increased shoot number per hypocotyl, regenerated shoot length, and total shoot number per petri dish in both cultivars. When explants were cultured at 0.5 cm spacing, significant stress-initiated decreases were observed in all parameters examined. This study showed that the success of tissue culture studies for related genotype could be increased not only by determination of correct concentrations and combinations of auxins and cytokinins in growth medium but also by evaluating competition among explants cultured.Keywords:
Linum
Petri dish
Rapid In Vitro Micropropagation of Two Different Cultivars of Phaseolus calcaratus RBL-1 and RBH-35.
A successful protocol for rapid proliferation of adventitious shoot from hypocotyl and leaf explants of two cultivars of Phaseolus calcaratus cv., RBL-1 and RBH-35 is described. A high frequency of multiple shoot buds was obtained directly from cut ends of the explants on MS medium supplemented with different (1-10 mgl-1) concentrations of auxins viz. 2, 4-dichlorophenoxyacetic acid (2, 4-D), indole-3-butyric acid (IBA) and naphthaleneacetic acid (NAA) alone and in combination with cytokinin, 6-benzyladenine (BA). Lower concentrations of auxins (0.1 and 0.5mgl-1) alone and in combination with different concentrations of BA were in general found to be the best for multiple shoot differentiation within 3-4 weeks of culture. Rooting of in vitro regenerated shoots was induced by NAA (0.1mgl-1) alone or a combination of IBA and BA (0.1+0.1mgl-1). Regeneration was much higher from hypocotyl than from leaf.
Organogenesis
Cite
Citations (0)
Micropropagation of tetraploid watermelon is important to cope with high cost of seed. Seeds of tetraploid watermelon were grown in vitro to raise seedlings. Hypocotyl and cotyledonary explants and media supplemented with plant growth regulators (BAP and NAA) was explored for callus induction and organogenesis. Data was collected for callus, shoot and root induction. Maximum callus induction was observed at BAP 5 mgL -1 (76.66%) from cotyledon and (73.33%) from hypocotyls explant. The callus induced from different explants was sub-cultured on the shoot regeneration medium. Higher shoot induction (96.66%) was observed from cotyledon and hypocotyl explant (76.66%) on MS + 1.0 mgL -1 BAP + 0.2 mgL -1 NAA with maximum number (6.3) of shoot per explant and average shoot length 4.5 cm. Among different types (NAA and IAA) and concentrations (0, 0.1, 0.3, 0.7, 1.0 mgL -1 ) of auxins investigated for root induction, maximum frequency of rooting was observed in 0.1 mgL -1 NAA while no root formation was observed at higher levels of auxin (1.0 mgL -1 ). Similarly in case of number of roots per shoot maximum root (4.3) was obtained on MS medium supplemented with 0.1 mgL -1 NAA. Key Words: Regeneration, Explant, Tetraploid, Water Mellon
Cotyledon
Callus
Organogenesis
Cite
Citations (5)
This study was carried out to investigate the relationship between in vitro competition and stress, and their effects on tissue culture response of Linum usitatissimum L. hypocotyl explants. Competition among explants was achieved by varying the spacing among the explants cultured. Four different culture spacing distances were used: 0.5, 1.0, 1.5, and 2.0 cm. Six weeks after culture initiation, hypocotyl fresh and dry weights, shoot regeneration percentage, shoot number per hypocotyl, regenerated shoot length, total shoot number per petri dish, and total chlorophyll content were recorded. The results showed that encouraging competition among explants by decreasing spacing among them from 2.0 cm to 1.0 cm increased shoot number per hypocotyl, regenerated shoot length, and total shoot number per petri dish in both cultivars. When explants were cultured at 0.5 cm spacing, significant stress-initiated decreases were observed in all parameters examined. This study showed that the success of tissue culture studies for related genotype could be increased not only by determination of correct concentrations and combinations of auxins and cytokinins in growth medium but also by evaluating competition among explants cultured.
Linum
Petri dish
Cite
Citations (22)
The present investigation was proposed to develop a reproducible and efficient regeneration protocol for organogenes is in tomato cv. Dhanashri. Seeds of tomato cv. Dhanashri were obtained from MP KV, Rahuri. The seedlings were raised aseptically on half-strength MS basal medium. Cotyledonary leaves and hypocotyls (1-2 cm), collec ted from 15days-old seedlings were excised and used as explants. Explan ts were cultured on MS basal medium supplemented with BAP (0.00-13.32 µM) and IAA(0.00 - 3.42 µM) alone and in various combinations. After 21-30 days when shoot buds were visible, shoots were separated from adhering cand transferred for induction of rooting on MS basal medium supplemente d with IBA (0.00-14.76 µM). The highest number of (7.8 ± 0.29) shoot per cleaf explant was produced on MS + 6.65 µM BAP in combination wit h 1.14 µM IAA, which was the most optimum combination for shoot regenera tion. The maximum numbers of shoots (5.6 ± 0.13) were observed on MS + 8.88 µM BAP in conjunction with 1.71 µM IAA. This combination was the optimum combination for induction of shoots from hypocotyl explants of tomato. Auxin containing medium (MS + 4.92 µM) resulted in a larg e proportion of rooted micro-shoots and early rooting. The results present ed describe an efficient, reproducible and rapid tissue culture regeneration protocol.
Lycopersicon
Organogenesis
Cite
Citations (4)
Effect of growth regulators on shoot morphogenesis in hypocotyl and cotyledonary leaf explants, shoot elongation and root induction has been studied. Hypocotyl explants showed callus initiation and direct regeneration response after 3–5 and 13–15 days of culture initiation, respectively. Cotyledonary leaf explants showed callus initiation and callus-mediated shoot regeneration response after 7–8 days and 3 weeks of culture initiation. Growth regulators did not affect callus initiation response but they markedly influenced regeneration response. Culture medium (full-strength MS salts, vitamins, 3% sucrose and 0.8% agar) containing 2 μM and 0.05 μM NAA induced highest shoot regeneration in hypocotyl explants (65.71%), whereas culture medium containing 10 μM BAP and 0.5 μM NAA induced highest 18.33% shoot regeneration in cotyledonary leaf explants. Either increase or decrease in BAP concentration than this resulted in reduction in shoot regeneration response. Addition of BAP to shoot elongation medium was found beneficial for shoot elongation and shoot regeneration medium was efficient in shoot elongation also. Addition of auxins in root induction medium has reduced number of days taken for root induction and increased in number of roots and root length. In hardening, 90–95% of rooted shoots survived and all the plantlets were morphologically normal.
Callus
Elongation
Cite
Citations (3)
Kinetin
Cotyledon
Zeatin
1-Naphthaleneacetic acid
Callus
Organogenesis
Cite
Citations (25)
Plantlet
Linum
Cite
Citations (35)
Hypocotyl explants of 3 flax cultivars were cultured for adventitious shoot regeneration in 3 different ways. Two pretreatment applications were compared with the routinely applied conventional regeneration protocol of culturing explants directly on Murashige and Skoog (MS) medium containing 1 mg L^{-1} 6-benzylaminopurine (BAP) and 0.02 mg L^{-1} naphthalene acetic acid (NAA). In the first pretreatment application, explants kept in a sterile cabin under an air flow for 30 min were immersed in MS solution containing 1 mg L^{-1} BAP and 0.02 mg L^{-1} NAA for 15 min; then pretreated explants were cultured on MS medium without any growth regulators (MS0). In the second pretreatment application, explants were kept in a sterile cabin under air flow for 30 min and then immersed in MS solution containing 1 mg L^{-1} BAP and 0.02 mg L^{-1} NAA for 15 min. The pretreated explants were then transferred to a culture medium enriched with 1 mg L^{-1} BAP and 0.02 mg L^{-1} NAA. Fresh and dry weights of hypocotyl explants, shoot regeneration percentage, shoot number per hypocotyl, shoot length, and total chlorophyll content were recorded. From the results, it could be seen that treating explants before culture initiation for regeneration with a liquid MS medium containing 1 mg L^{-1} BAP and 0.02 mg L^{-1} NAA for 15 min after keeping hypocotyls under an air flow in a sterile cabin for 30 min gave rise to the highest scores for tissue culture response.
Linum
Cite
Citations (8)
Organogenesis
Callus
Basal shoot
Cite
Citations (30)