[JAK3 mRNA level in graft versus host disease patients: a preliminary study].

Objective To quantify peripheral blood mononuclear cell JAK3 mRNA levels after allogeneic haematopoietic stem cell(allo-HSCT)transplantation,and to explore its relationship with graft vs host disease(GVHD).Methods Serial monitoring of JAK3 mRNA levels by fluorescent quantitative reverse transcriptase polymerase chain reaction(FQ-RT-PCR)technique was performed on 26 cases(83 samples)after allo-HSCT.Results The mean JAK3/ABL expression levels was 8.71 in 14 patients without GVHD;the JAK3/ABL expression level was 31.94 in one patient with acute GVHD(aGVHD);the mean JAK3/ABL expression levels was 19.23 in 11 patients with chronic GVHD(cGVHD),and the cGVHD was diagnosed at the mean time of six months(3-12 months).The JAK3/ABL expression levels did not change in 4 patients one month after allo-HSCT,however,the levels increased again when GVHD were diagnosed in these 4 patients,and the JAK3/ABL expression levels decreased again when GVHD was remitted. Conclusion The JAK3 expression level related to the remission and relapse in patients with GVHD.