Analysis of the Dynamical Biological Objects of Optical Microscopy

We generalize the experience of the applied and theoretical investigations performed in the last 7‒8 years, including the generated algorithms and procedures for analyzing the behavior of the cells’ population as a system of dynamical objects by using a concept of the integral optical flow. We determine the main types of motion which make it possible to separate the key moments of the motion of cells in the population and describe the stages of the cells’ development and their interaction with each other. In order to describe the motion based on the integral optical flow, we generate maps which make possible to determine the moment at which the state starts to change and classify the main types of motion in the cells’ population: directed motion, aggregation (cells motion towards each other), dispersion (cells’ motion in different directions with respect to the common center), division (formation of several new cells in the place where the old one was), and apoptosis (cell destruction). We develop the algorithms for analyzing cells’ motion by using the integral optical flow. The generated procedure is intended for nondestructively checking the development of the cells’ population and it can be used in automatic systems of cell engineering. The procedure based on integral optical flow analysis and motion maps makes it possible to monitor the development of the cells’ population at the new level by considering the interaction of cells.