Stem cell–related gene expression in clonal populationsof mesenchymal stromal cells from bone marrow
2010
Decline in the frequency of potent mesenchymal stem cells (MSCs) has been implicated in ageing and degenerative
diseases. Increasing the circulating stem cell population can lead to renewed recruitment of these potent cells
at sites of damage. Therefore, identifying the ideal cells for ex vivo expansion will form a major pursuit of clinical
applications. This study is a follow-up of previous work that demonstrated the occurrence of fast-growing multipotential
cells from the bone marrow samples. To investigate the molecular processes involved in the existence of
such varying populations, gene expression studies were performed between fast- and slow-growing clonal populations
to identify potential genetic markers associated with stemness using the quantitative real-time polymerase
chain reaction comprising a series of 84 genes related to stem cell pathways. A group of 10 genes were
commonly overrepresented in the fast-growing stem cell clones. These included genes that encode proteins
involved in the maintenance of embryonic and neural stem cell renewal (sex-determining region Y-box 2, notch
homolog 1, and delta-like 3), proteins associated with chondrogenesis (aggrecan and collagen 2 A1), growth
factors (bone morphogenetic protein 2 and insulin-like growth factor 1), an endodermal organogenesis protein
(forkhead box a2), and proteins associated with cell-fate specification (fibroblast growth factor 2 and cell division
cycle 2). Expression of diverse differentiation genes in MSC clones suggests that these commonly expressed genes
may confer the maintenance of multipotentiality and self-renewal of MSCs.
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