Modulation of sterol regulatory element binding protein-2 in response to rapid follicle development in chickens

Abstract We investigated the expression profiles of sterol regulatory element binding proteins (SREBPs) and their related genes in chicken developing follicle membranes, from the small white follicle (SWF) stage to the Follicle 1 (F1) stage. Expression of SREBP-2 was significantly increased in the rapid stages of follicle development, however, no significant change in SREBP-1 mRNA expression was observed during follicle development. Immunoreactive SREBP-2 protein levels isolated from nuclear extracts in rapid growth stages, particularly in Follicle 2, were higher than those in SWF and small yellow follicle (SYF). In contrast, SREBP-1 immunoreactive protein levels were only slightly changed over all stage of follicle development. 3-Hydroxy-3-methylglutaryl CoA reductase (HMGR) mRNA levels significantly increased in the rapid stages of follicle development, suggesting that SREBP-2 controls the biosynthesis of cholesterol in follicles. LDL receptor and LDL receptor related protein 1 mRNA also tended to increase with follicular development, however, expression of LDL receptor relative with eight ligand binding repeats (LR8) was only slightly affected by SREBP-2. Liver X receptor α (LXR α) was expressed in chicken follicles; its expression patterns corresponded with SREBP-2 gene expression. These results suggest that SREBP-2, which might be regulated by LXRα, is involved in the rapid growth stages of follicle development in avian species.