Abstract 14707: Functional Cardiac Regeneration by Statin-nanoparticle Loaded Stem Cells in Myocardial Infarction

Introduction: Clinical trials of autologous adipose-derived stem cell (AdSC) therapy for ischemic heart diseases (IHD) are now on-going, however, transplantation of small number of AdSCs has been reported to diminish its therapeutic effect. Hypothesis: We have investigated the hypothesis that combination of small number of AdSCs and statin, an agent with pleiotropic effects, could augment the AdSC functions and therapeutic effect on myocardial infarction (MI). Methods: AdSCs were enzymatically isolated from human adipose tissue and cultured for expansion. Statin-conjugated nanoparticle (STNP) was made with simvastatin and biodegradable polymer, (co-poly lactic acid/glycolic acid, PLGA), and was loaded into the cultured AdSCs. The cell functions with different doses of STNP uptake were evaluated by in vitro assays. MI was induced by LAD ligation in nude mice, and the mice were assigned in the following groups with different treatments by tail vein injection 3 days after MI: 1) PBS (control), 2) AdSCs (1x10...