Multiplexing nanostructured plasmonic device for high throughput biosensing

The fabrication and biological application of a grating-based plasmonic lab-on-chip aimed at the parallel detection of multiple reactions are here demonstrated. This work answers to the recent biosensing requirements of high throughput and compact detection equipment. Laser interference lithography (LIL), UV photolithography and metal etching techniques were combined to realize a compact device able to support up to 100 biological reactions in a 1 mm2 sensing area. Avidin physisorption onto the sensing surface was used as model reaction. We here show the potentialities of the system by monitoring four biological events in parallel.