Multifunctional Detection of Extracellular Vesicleswith Surface Plasmon Resonance Microscopy
2020
Extracellular vesicles
(EVs), including exosomes, are promising
circulating biomarkers for disease diagnosis. Conventional EVs analysis
requires multiple instrumentations to obtain their phenotypic features,
which limits its wide applications. Here, we present a plasmonic biosensor
technology for multifunctional analysis of EVs. The system is based
on a functionalized surface plasmon resonance (SPR) biosensor and
an advanced plasmonic microscopy to capture and image EVs at single-particle
level. SPR images are processed with a home-developed deep learning
algorithm to identify EVs and quantify image intensity automatically.
By combining immunosensing and single particle analysis, this approach
enables both physical and chemical characterization of EVs. As a proof-of-concept,
we applied it to analyze EVs secreted from human lung cancer A549
cell lines. Results show the capabilities in the detection of size,
concentration and affinity constant. Due to the single particle imaging
and multifunctional analysis capability, we anticipate that this technology
will find use in clinical and scientific applications.
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