Photodetection of Micro-Biomotility and Ecological Monitoring of Lower Organisms

2013 
A method is proposed for the ecological monitoring of lower organisms by using the frequency of mechanical vibrations, and the real time detection of their motion. The synchronous mechanical oscillations of invertebrates were detected under constant frequency bands. The waves generated by these organisms in their normal state were observed, as well as the vibrations splitting response under various external conditions such as: a magnetic field gradient and thermal stress. The spectroscopic analysis of the intensity fluctuations of light scattered by the organisms allowed the detection of complex biorhythms. Used optical portable spectrometer detected Fourier spectra and real-time rhythms of cilia beating and drifting motility. The common main cilia beating frequency was found between 16-17 Hz for Artemia Salina brine shrimps. The experimental results demonstrated that the reaction of cilia beating to a gradient of a magnetic field is weaker than the reaction to other stress induced by temperature gradients.
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