STUDIES REGARDING THE IN VITRO WOUND HEALING POTENTIAL OF MOUSE DENTAL PULP STEM-LIKE PROGENITOR CELLS

ABSTRACTWound healing involves complex interactions between the motile epithelial cells and the extracellular matrix in response to activation by a high number of growth factors and cytokines involved both in inflammatory and wound healing process. In the present study a series of events which occur during the wound healing process in vivo were reproduced in vitro. The artificial wound was induced on a mouse dental pulp stem-like progenitor cell monolayer in addition to a series of factors involved in the inflammatory and re-epithelization process (TNF-alpha and FGF). At the same time the cell-matrix interactions using different surface coatings like: laminin, fibronectin and matrigel, which are playing a major role in cell adhesion, growth, migration and wound healing process, were observed. The results were compared to similar experiments on mouse dermal fibroblasts. For dental pulp stem-like progenitor cell high wound closure percentage was obtained on matrigel coating TNF-alpha treated cells (95% woun...