In utero hematopoietic stem cell transplantation with haploidentical donor adult bone marrow in a canine model

Abstract Objective Chimerism can be achieved in a canine model of in utero bone marrow transplantation with ≥1×10 8 CD34 + haploidentical donor cells per kilogram without graft-versus-host disease. Study design In utero bone marrow transplantation was performed by ultrasound-guided intraperitoneal infusion in 30- to 41-day-old canines with CD34 + selected cells from paternal bone marrow at doses of 1.3×10 8 to 2.5×10 10 CD34 + cells/kg. A method for marking control littermates was developed with intraperitoneal ethiodol. Postnatal studies included histologic, fluorescent in situ hybridization canine Y probe, and polymerase chain reaction–based chimerism analyses. Results Term survival was 86% to 100% for transplantations ≥34 days versus 14% and 43% at 30 and 31 days. Microchimerism ( Conclusion In utero bone marrow transplantation in a canine model achieves microchimerism with high CD34 + cell doses.