ICSI with All Oocytes Recurrent Metaphase I Characterized by Absence Perivitelline Space

Previous cases reported recurrent all oocytes in repeated cycles were arrested at MI failed to mature after culture and ICSI resulted in total nonfertilization. Ionomycin induced first PB extrusion in patients with recurrent maturation-resistant MI oocytes. ICSI combined with assisted oocyte activation (AOA) on MII oocytes matured in vitro resulted in abnormal fertilization with no further embryo cleavage potential. The objective of this work was to research if AOA helpful to fertilization for recurrent maturation-resistant MI oocytes. In this case, patient both cycles retrievaled immature MI oocytes characterized by absence perivitelline space (APVS). In the second cycle, ionomycin was used for AOA after MI oocyte perfomed ICSI, no fertilization again.