Mannheimia haemolytica pleuropneumonia in goats associated with shipping stress

portuguesDescreve-se os aspectos epidemiologicos, clinicos, patologicos e bacteriologicos da pleuropneumonia por Mannheimia haemolytica em caprinos, apos transporte prolongado. Quarenta caprinos transportados da regiao Nordeste para a regiao Sul do Brasil morreram durante a viagem ou 2-3 dias apos o desembarque. Clinicamente, observou-se dispneia, secrecao nasal mucopurulenta e tosse. Na necropsia foram coletados multiplos fragmentos de orgaos para analises histopatologicas e identificacao do agente envolvido. Todos os pulmoes apresentaram consolidacao pulmonar predominantemente em regiao cranioventral, associada a deposicao acentuada de fibrina, espessamento e aderencia pleurais em 90% dos casos. Histologicamente, havia pleuropneumonia fibrinossupurativa caracterizada por infiltrado neutrofilico difuso associado a fibrina. Colonias nao hemoliticas [85% (34/40)] e hemoliticas [15% (6/40)] foram obtidas pelo isolamento bacteriologico. Mannheimia spp. foi isolada em 26 amostras, os quais posteriormente foram confirmados como Mannheimia haemolytica (99% de identidade), pela amplificacao e sequenciamento parcial do gene 16S rDNA. O estresse pode favorecer o desenvolvimento de pleuropneumonia bacteriana em caprinos, e cepas nao hemoliticas de M. haemolytica podem causar doenca em animais com imunodepressao acentuada. Palavras-chave: doencas de caprinos; doencas respiratorias; patologia; PCR EnglishThis study described the epidemiological, clinical, pathological and bacteriological aspects of Mannheimia haemolytica pleuropneumonia in goats associated with shipping stress. Forty goats transported from the Northeast to the Southern region of Brazil died during shipment, or 2-3 days after unloading. Clinical signs included dyspnea, mucopurulent nasal discharge, and coughing. All goats were necropsied, and multiple tissues were collected for histopathological analysis and involved agent identifications. All lungs showed pulmonary consolidation, predominantly affecting the cranioventral lobes, in addition to the marked fibrinous pleuritis, pleural thickening, and pleural adhesions, that affected 90% of the goats. Histologically, there was fibrino-suppurative pleuropneumonia characterized by a diffuse neutrophilic infiltrate admixed with fibrin. Non-hemolytic [85% (34/40)] and hemolytic [15% (6/40)] species were obtained in bacteriological culture. Fir Mannheimia spp. was reported in 26 isolates and subsequently confirmed as M. haemolytica (99% identity), after amplification and partial sequencing of the 16S rDNA gene. Stress may trigger the development of bacterial pleuropneumonia in goats, and non-hemolytic strains of M. haemolytica may cause this condition in goats with severe immunosuppression. Key words: goat diseases; respiratory diseases; pathology; PCR