Formulation and Evaluation of Polyherbal Wound Healing Ointment

Aim: The present investigation was aimed to formulate and evaluate the polyherbal wound healing ointment constituting the methanolic extracts of Lantana camara (leaves), Tamarindus indicus (leaves), Psidium guajava (leaves) and acetone extract of Curcuma longa (rhizomes). Materials and Methods: Two Formulations (F1, F2) were formulated by using fusion method using a mixture of PEG 4000 and PEG 600 as ointment base. The physical evaluation of these polyherbal formulations were made in terms of physical and chemical parameters like pH, physical stability, centrifugation, spreadability, viscosity, extrudability. Assessment of wound healing activity was made by using excision wound model in which the evaluation parameters were wound contraction and epithelialization period in excision wound model by using healthy Albino wister rats. In this model, the animals were divided into 4 groups (n = 6) named as control, standard, formulation1 (F1), formulation2 (F2) receiving vehicle (ointment base), soframycin (2%w/w), F1, F2 respectively. The measurement of wound areas were taken on 3rd, 6th, 9th, 12th and 15th day following the initial day of wound. The percentage of reduction in wound area, percentage of protection and period of epithelialization was calculated Statistical analysis: Data were analysed by using one way ANOVA followed by Dunnett’s comparison test. Results: The results were found to be significantly positive (p<0.05) with the F1, F2. More over F1 shows more significant positive result than F2 when compare to standard. Conclusion: The results of this study reveals the synergetic effect of the phytoconstituents like tannins, saponins, curcumin, alkaloids present in those plants extracts which help to establish these formulations clinically effective in the wound healing management.