A Study of the Natural Coagulating Inhibitors Polymorphism in Iranian Patients with the Vine Thrombosis

Introduction: Anti thrombin III (AT-III), protein C (PC), protein S (PS) are named the natural coagulation inhibitors, quantitative and qualitative defects of these inhibitor proteins caused recurrent veins thrombosis. Despite there are many clinical and technical assays for improving diagnosis of this disease, but diagnosis and classification of this disease facing with some challenges. The diagnosis polymorphism and mutation of PC, PS, and Anti-III, recognizing the homozygote and heterozygote form in people who have any patient with vein thrombosis in their family. Materials & Methods: 42 patients with vascular thrombosis, who visited cardiovascular and thromboses clinic of Immam khomaini and Shahid Modarres General Hospitals have been studied in this research. After performing coagulation test and determining the activity of anti coagulation proteins (PC, PS, Anti-III), The DNA of patients samples were extracted and reproduce by PCR. Pieces of DNA upon reproduction sent to Biosciences Co in UK to determining its sequence. DNA sequences were comprised with wild type of proteins genome. Results: The activities of PS in 63.5%, PC in 29.2 and AT-III in 7.3% patients were reduced. There was a statistical relation between reducing plasma activity of PC and PS of patients and their familial thrombosis (P< 0.05). Results obtained from Chromas software showed that there are remarkable polymorphisms in some patients who are suffered from abnormal of related proteins. Discussion & Conclusion: Although, many studies are performed in development countries for recognizing this disease, there is not a lot of study which carried out in Iran about this issue. Therefore, it is required to perform more molecular tests on natural coagulation inhibitors and other effective proteins on creation of vascular thrombosis.