Structural characterization and anticoagulant activity of two polysaccharides from Patinopecten yessoensis viscera

Abstract In the present study, two polysaccharides, SVP2–1 and SVP2–2, were isolated from Patinopecten yessoensis viscera and purified by using DEAE-52 cellulose and Sepharose CL-6B. Both SVP2–1 and SVP2–2 could extend activated partial thromboplastin time (APTT) and thrombin time (TT) and inhibit the transformation of fibrinogen into fibrin (FIB) concentration-dependently, indicating they inhibited clotting and thrombin through intrinsic and common pathways. Of note, SVP2–2 had stronger anticoagulant activity than SVP2–1, and its backbone was determined as →6)-α-Man p (1 → 2)-α-Gal p (1 → with Xyl or Glc substituted at C4 of Gal. Based on monosaccharide composition analysis, methylation analysis, and NMR analysis. Further comparison of their monosaccharide analysis and NMR spectra indicates SVP2–1 and SVP2–2 possess the same core structure features, so the higher sulfate content and lower molecular weight may be the possible reasons for the stronger anticoagulant capability of SVP2–2. The present study suggests acidic polysaccharides from scallop viscera as promising anticoagulant candidates.