Flow Cytometry: A Rapid and Robust Adjuvant Technique for Pathological Diagnosis

.Immunophenotyping was performed using CD45, CD19, CD3, CD5, CD7, CD4, CD8, CD38, CD56, kappa, and lambda light chains. gating was performed using for-ward-side scatter and CD45-side scatter.A single large cluster observed in the lymphocyte region of the SSC/CD45 side scatter showed a high level of CD45 expression and was composed predominantly of T-cells (CD3+) with moderately high CD38 positivity (T-cell activation marker). nothing abnormal with respect to T-cell markers (CD3/CD5/CD7) was noted. reversal of the CD4:CD8 ratio (0.1) was also noted. CD19, kappa, lambda, and CD56 expression was not observed. Immu-nophenotyping results suggested non-neoplastic prolifera-tion of lymphocytes—primarily involving a T-cell compo-nent with an inverted CD4:CD8 ratio, which could also be a manifestation of immunosuppressive states and viral infections, e.g. EBv, Cmv, and HIv [1]. EBv Igm was positive (17.58 U ml