Method and kit for simultaneous detection of human coronavirus 229E, OC43 and NL63

The invention belongs to the field of biological technical application, and relates to a TaqMan probe based multiple fluorescent RT-PCR method for simultaneous detection of human coronavirus 229E, OC43 and NL63 and containing internal quality control. The invention designs specific primers and probed aiming at conserved sequences of N gene of human coronavirus representative strains 229E, OC43 and NL63, and establishes a one-step multiple fluorescent RT-PCR rapid detection method containing internal quality control; the method has simple and quick operations, overcomes the complexity of single hole single detection in a conventional single fluorescent RT-PCR method, simplifies the operation process, saves test cost, and provides a powerful technical support for on-site virus detection, health assessment and clinical diagnosis by using high specificity, sensitivity, efficiency and stability.