NS5ATP9 gene regulated by NF-κB signal pathway

Abstract NS5ATP9 was previously identified as p15 PAF [proliferating cell nuclear antigen (PCNA)-associated factor] to bind with PCNA. We earlier identified the promoter region of NS5ATP9 and found NS5ATP9 is a NS5A up-regulation gene. However little is known about how it is regulated. To investigate the gene regulation of NS5ATP9 , we screened NS5ATP9 promoter binding proteins using phage display and verified by electrophoretic mobility shift assay (EMSA). We found that the nuclear protein rhNF-κB (p50) could bind to the NS5ATP9 promoter and the binding region contained within a 156 bp (nucleotides −5 to −161 bp) immediately upstream of the transcription initiation site. Our results suggest that NF-κB could participate in the regulation of NS5ATP9 gene expression in carcinogenesis.